Homoeologous cloning of ω-secalin gene family in a wheat 1BL/1RS translocation

Chai, Jianmin; Xu, Liu; Jia, Ji Zeng

doi:10.1038/sj.cr.7290335

Cited by 19 publications

(17 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The oligonucleotide primers used in this work are listed in Supplementary Table S1. General methods for molecular cloning and PCR were as described previously (Chai et al. , 2005).…”

Section: Methodsmentioning

confidence: 99%

Molecular analysis of three new receptor‐like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection

et al. 2007

View full text Add to dashboard Cite

SummaryConsiderable progress has been made in understanding the function of receptor-like kinase (RLK) genes in model plants. However, much less is known about these genes in crop species. Here we report the characterization of three new wheat RLK genes (TaRLK-R1, 2 and 3). The primary structure of the putative proteins TaRLK-R1, 2 and 3 contained a signal peptide, a cysteine-rich extracellular domain, a transmembrane domain, and a predicted intracellular kinase domain. The fusions between TaRLK-R1, 2 or 3 and the green fluorescence protein (GFP) were targeted to the plasma membrane; such targeting required the signal peptide, extracellular domain and transmembrane domain. Transcription of TaRLK-R1, 2 and 3 was found mainly in the green organs, and was regulated by light. Transcript levels of TaRLK-R1, 2 and 3 increased during the hypersensitive reaction (HR) to stripe rust fungus. In addition, the TaRLK-R3 transcript level was also upregulated by abiotic stresses. Further experiments revealed that the recombinant kinase domain of TaRLK-R3 exhibited auto-phosphorylation activity in vitro. Knocking down the transcript levels of TaRLK-R1, 2 or 3 individually or all together by virus-induced gene silencing compromised the wheat HR to stripe rust fungus. The demonstration of TaRLK-R1, 2 and 3 as positive contributors in the wheat HR to stripe rust fungus suggests a new direction for further functional studies of this important family of RLK genes, and may facilitate the breeding of wheat varieties resistant to stripe rust disease.

show abstract

“…The oligonucleotide primers used in this work are listed in Supplementary Table S1. General methods for molecular cloning and PCR were as described previously (Chai et al. , 2005).…”

Section: Methodsmentioning

confidence: 99%

Molecular analysis of three new receptor‐like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The secalin genes on the short arm of rye chromosome 1R are well characterized as a cluster of paralogous sequences, consisting of approximately 15 copies spanning a physical distance of at least 145 kb (Clarke and Appels 1999;Chai et al 2005;Yamamoto and Mukai 2005). The seed storage protein profiles indicated that all copies of the ex-pressed secalin gene complex were eliminated in line T6-1, and thus this line may provide improvements in dough quality characteristics (Kumlay et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Isolation of wheat–rye 1RS recombinants that break the linkage between the stem rust resistance gene SrR and secalin

Anugrahwati

Shepherd

Verlin

et al. 2008

Genome

View full text Add to dashboard Cite

Chromosome 1R of rye is a useful source of genes for disease resistance and enhanced agronomic performance in wheat. One of the most prevalent genes transferred to wheat from rye is the stem rust resistance gene Sr31. The recent emergence and spread of a stem rust pathotype virulent to this gene has refocused efforts to find and utilize alternative sources of resistance. There has been considerable effort to transfer a stem rust resistance gene, SrR, from Imperial rye, believed to be allelic to Sr31, into commercial wheat cultivars. However, the simultaneous transfer of genes at the Sec-1 locus encoding secalin seed storage proteins and their association with quality defects preclude the deployment of SrR in some commercial wheat breeding programs. Previous attempts to induce homoeologous recombination between wheat and rye chromosomes to break the linkage between SrR and Sec-1 whilst retaining the tightly linked major loci for wheat seed storage proteins, Gli-D1 and Glu-D3, and recover good dough quality characteristics, have been unsuccessful. We produced novel tertiary wheat-rye recombinant lines carrying different lengths of rye chromosome arm 1RS by inducing homoeologous recombination between the wheat 1D chromosome and a previously described secondary wheat-rye recombinant, DRA-1. Tertiary recombinant T6-1 (SrR+ Sec-1-) carries the target gene for stem rust resistance from rye and retains Gli-D1 but lacks the secalin locus. The tertiary recombinant T49-7 (SrR- Sec-1+) contains the secalin locus but lacks the stem rust resistance gene. T6-1 is expected to contribute to wheat breeding programs in Australia, whereas T49-7 provides opportunities to investigate whether the presence of secalins is responsible for the previously documented dough quality defects.

show abstract

“…The incorporation of this locus into wheat had a negative effect on wheat processing quality (Miller, 1984;Shewry et al, 1986). Sec1 is a complex locus with families of closely linked genes (Chai et al, 2005;Jiang et al, 2010). A section encoding u-secalins in this locus has been sequenced and shown to contain about 15 u-secalin genes arranged in a head to tail fashion (Clarke et al, 1996).…”

Section: Introductionmentioning

confidence: 97%

Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye

Wang

Zhang

Liu

et al. 2012

Journal of Cereal Science

View full text Add to dashboard Cite

Homoeologous cloning of ω-secalin gene family in a wheat 1BL/1RS translocation

Cited by 19 publications

References 17 publications

Molecular analysis of three new receptor‐like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection

Molecular analysis of three new receptor‐like kinase genes from hexaploid wheat and evidence for their participation in the wheat hypersensitive response to stripe rust fungus infection

Isolation of wheat–rye 1RS recombinants that break the linkage between the stem rust resistance gene SrR and secalin

Structure and evolutionary relationships among paralogous genes within the Sec2 locus in rye

Contact Info

Product

Resources

About