Homoeologous chromosome pairing at metaphase I of meiosis in &lt;i&gt;Hordeum vulgare&lt;/i&gt; L. × &lt;i&gt;H. bulbosum&lt;/i&gt; L. triploid hybrids (HvHbHb)

Pendinen, Galina; Scholz, Margret

doi:10.30901/2658-6266-2020-2-o2

Cited by 2 publications

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“…В ряде комбинаций скрещиваний при соотношении плоидности родительских видов 1Hv : 1Hb с использованием сортов ячменя 'Emir' (2х) (Zhang et al, 1999;Pickering et al, 2004;2006а) и 'Borwina' (4х) получены цитогенетически стабильные гибриды (Szigat, Pohler, 1982, Scholz, Pendinen, 2017. При соотношении геномов родительских видов 1Hv : 2Hb в скрещиваниях тетраплоидного H. bulbosum с диплоидным H. vulgare элиминации хромосом ячменя луковичного не происходит и в потом-стве наблюдаются цитогенетически стабильные триплодные гибриды (HvHbHb) независимо от используемых для создания гибридов образцов родительских видов (Lange, 1971a;Yu, 2018;Pendinen, Scholz, 2020).…”

Section: Introductionunclassified

Possibilities of using the tetraploid interspecific hybrid Hordeum bulbosum L. × Hordeum vulgare L. in obtaining new recombinant barley lines

Pendinen,

Chernov,

Scholz

2024

Biotehnologiâ i selekciâ rastenij

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Background. Hordeum bulbosum L. is the only wild species of the genus Hordeum, the gene pool of which is successfully used in introgressive hybridization to increase the genetic diversity of H. vulgare L. When creating introgression forms based on hybrids of H. vulgare with H. bulbosum, it is necessary to take into consideration the ploidy of the parent species, and their genetic features that determine the intensity of the process of H. bulbosum chromosomes elimination in embryogenesis. The purpose of our investigation was to study the features associated with the elimination of bulbous barley chromosomes in embryos obtained in various combinations of crosses involving the tetraploid hybrid H. bulbosum with H. vulgare for its effective use in obtaining introgression lines of H. vulgare. Material and methods. The analysis was performed on 9-15 days old embryos obtained in crosses of the tetraploid hybrid F2 (H. bulbosum A17 (4x) × H. vulgare ‘Borwina’ (4x)) (HbHbHvHv) with ‘Igri’ (2x) and ‘Borwina’ (2x) barley varieties, with H. bulbosum A17 (4x), as well as during its self-pollination. The chromosomal composition of embryos of different ages was analyzed on squashed embryo slides using genomic in situ hybridization (GISH). Results. Among the 11-15 days old embryos obtained from self-pollination of the hybrid, approximately half of the studied ones were mixoploids, while in other embryos the majority of cells contained only the genomic material of H. vulgare. The elimination process was very intensive in crosses of the hybrid with the barley varieties ‘Igri’ (2x) and ‘Borwina’ (2x), and by day 10-11 after pollination cells containing only the genomic material of H. vulgare predominated in all embryos, regardless of the direction of crossing and the variety used. Hybrid embryos with a stable chromosomal composition resulted from a cross of a tetraploid hybrid with H. bulbosum A17 (4x). Embryos with recombinant H. vulgare chromosomes carrying alien genetic material of bulbous barley were identified in all types of crosses. Conclusion. The most efficient way for the mass production of introgression lines of cultivated barley varieties based on the partially fertile hybrid F2 (H. bulbosum A17 (4x) × H. vulgare ‘Borwina’ (4x)), is the crossing with varieties of H. vulgare (2x). The progeny from such crosses will contain only cultivated barley plants, among which it is possible to identify forms with the introgression of H. bulbosum and use already the first generation from their self-pollination for selecting H. vulgare lines carrying the genetic material of bulbous barley in both homologs.

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Section: Introductionunclassified

Possibilities of using the tetraploid interspecific hybrid Hordeum bulbosum L. × Hordeum vulgare L. in obtaining new recombinant barley lines

Pendinen,

Chernov,

Scholz

2024

Biotehnologiâ i selekciâ rastenij

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New introgressive forms of cultivated barley obtained on the basis of interspecific hybrids Hordeum vulgare L. × Hordeum bulbosum L.

Pendinen

2022

Biotehnologiâ i selekciâ rastenij

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Background. The use of alien genetic material of bulbous barley Hordeum bulbosum L. to increase the diversity of cultivated barley Hordeum vulgare L. is an important task, since H. bulbosum is characterized by a number of valuable traits. One of the ways to use the genetic potential of bulbous barley is the interspecific hybridization and obtaining fertile introgressive lines of H. vulgare based on interspecific hybrids. The aim of the study was to obtain new introgressive forms of spring barley using interspecific triploid (HvHbHb) and tetraploid (HbHbHvHv) hybrids of cultivated barley with bulbous barley to expand the collection of introgressive lines of H. vulgare. Materials and methods. To create new introgressive forms, diploid barley H. vulgare (2x) cv. ‘Roland’, was crossed with interspecific hybrids H. vulgare cv. ‘Roland’(2x) × H. bulbosum W851 (4х) (HvHbHb), and H. bulbosum A17 (4x) × H. vulgare ‘Borwina’ (4x) (HbHbHvHv). Cultivated barley forms with introgression of the bulbous barley genetic material were selected from the offspring from these crosses; then the selection was continued in two progenies from self-pollination of selected BC1 plants with three alien introgressions. Identification and localization of introgressions was carried out using the method of fluorescent in situ hybridization (GISH and FISH with chromosome-specific markers). Results. The crossing of cultivated barley with triploid and tetraploid interspecific hybrids H. vulgare × H. bulbosum yielded new forms of cultivated barley with recombinant chromosomes, among which two plants with three terminal introgressions of the genetic material of bulbous barley were identified. The first plant, derived from a triploid hybrid, showed introgressions in chromosomes 5HL, 1HL and 3HS. When two homologues with the 5HL introgression of the initial size are combined in the karyotype, the lethality of seedlings is observed in the offspring plants. In the second plant obtained on the basis of a tetraploid hybrid introgression was revealed in chromosomes 5HL, 2HL, and 7HS. In the offspring from self-pollination of this form, the presence of the 2HL introgression of the original size in both homologues led to plant sterility. Forms with a change in size of the introgression in 5HL and 3HS in the offspring of the first plant and with a change in size of the introgression in 2HL in the offspring of the second plant were detected, which indicated that meiotic recombination had occurred in those chromosome regions in BC1 plants. Conclusions A barley plant with the introgression of bulbous barley chromatin into chromosomes 1HL, 5HL, and 3HS of cultivated barley was identified in the offspring from a cross of cultivated barley with a triploid interspecific hybrid H. vulgare × H. bulbosum. In crosses with a tetraploid interspecific hybrid, a barley plant with the introgression into chromosomes 2HL, 5HL, and 7HS was found. On the basis of ‘Roland’ spring barley cultivar, two series of new introgressive forms of H. vulgare with various combinations of recombinant chromosomes have been created.

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Homoeologous chromosome pairing at metaphase I of meiosis in <i>Hordeum vulgare</i> L. × <i>H. bulbosum</i> L. triploid hybrids (HvHbHb)

Cited by 2 publications

References 31 publications

Possibilities of using the tetraploid interspecific hybrid <i>Hordeum bulbosum</i> L. × <i>Hordeum vulgare</i> L. in obtaining new recombinant barley lines

Possibilities of using the tetraploid interspecific hybrid <i>Hordeum bulbosum</i> L. × <i>Hordeum vulgare</i> L. in obtaining new recombinant barley lines

New introgressive forms of cultivated barley obtained on the basis of interspecific hybrids <i>Hordeum vulgare</i> L. × <i>Hordeum bulbosum</i> L.

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