Homodimerization of HYL1 ensures the correct selection of cleavage sites in primary miRNA

Yang, Xi; Ren, Wenqing; Zhao, Qiuxia; Zhang, Peng; Wu, Feijie; He, Yuqing

doi:10.1093/nar/gku907

Cited by 58 publications

(60 citation statements)

References 54 publications

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Section: Microrna Biogenesis In Plantsmentioning

confidence: 99%

“…HYL1 interacts with DCL1 to facilitate efficient and precise miRNA precursor processing (Kurihara et al ., 2006; Dong et al ., 2008; Manavella et al ., 2012a; Yang et al ., 2014). Homodimerization of HYL1 is essential for its functions in miRNA precursor processing (Yang et al ., 2010,2014). HYL1 also affects the splicing of some pri-miRNAs and strand selection from miRNA/miRNA* duplexes in AGO1 loading (Szarzynska et al ., 2009; Manavella et al ., 2012a; Ben Chaabane et al ., 2013).…”

Section: Microrna Biogenesis In Plantsmentioning

confidence: 99%

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The ‘how’ and ‘where’ of plant microRNAs

2017

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Contents 1002I.1002II.1007III.1010IV.10131013References1013 Summary MicroRNAs (miRNAs) are small non‐coding RNAs, of typically 20–24 nt, that regulate gene expression post‐transcriptionally through sequence complementarity. Since the identification of the first miRNA, lin‐4, in the nematode Caenorhabditis elegans in 1993, thousands of miRNAs have been discovered in animals and plants, and their regulatory roles in numerous biological processes have been uncovered. In plants, research efforts have established the major molecular framework of miRNA biogenesis and modes of action, and are beginning to elucidate the mechanisms of miRNA degradation. Studies have implicated restricted and surprising subcellular locations in which miRNA biogenesis or activity takes place. In this article, we summarize the current knowledge on how plant miRNAs are made and degraded, and how they repress target gene expression. We discuss not only the players involved in these processes, but also the subcellular sites in which these processes are known or implicated to take place. We hope to raise awareness that the cell biology of miRNAs holds the key to a full understanding of these enigmatic molecules.

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Section: Microrna Biogenesis In Plantsmentioning

confidence: 99%

Section: Microrna Biogenesis In Plantsmentioning

confidence: 99%

The ‘how’ and ‘where’ of plant microRNAs

2017

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“…Both HYL1 and SE participate in the different biogenesis modes of plant miRNAs, as mutations in their genes result in a global decrease of miRNA levels (4,5,8,9). These proteins have been implicated in several processes including processing precision (12,14,16,47,48), strand selection (49) and splicing (10,50). In vitro processing studies using MIR167b and MIR166g precursors have shown that DCL1 generates more incorrect cuts in the absence of HYL1, especially near the end of the transcript (12,14).…”

Section: Discussionmentioning

confidence: 99%

“…Resulting MIRNA primary transcripts are then recognized by a dicing complex, that harbors DICER-LIKE1 (DCL1) and the core components HYPONASTIC LEAVES 1 (HYL1), a dsRNA binding protein (4)(5)(6)(7) and the zinc finger domain SERRATE (SE) (8)(9)(10)(11). DCL1, HYL1 and SE interact in vivo and are required for an efficient and precise processing of miRNA precursors (12)(13)(14)(15)(16)(17). Several other factors have been shown to aid on miRNA biogenesis modulating the final levels of the small RNAs [reviewed in (1)].…”

Section: Introductionmentioning

confidence: 99%

Efficiency and precision of miRNA biogenesis modes in plants

Moro

Chorostecki

Arikit

et al. 2018

Preprint

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Many evolutionarily conserved microRNAs (miRNAs) in plants regulate transcription factorswith key functions in development. Hence, mutations in the core components of the miRNA biogenesis machinery cause strong growth defects. An essential aspect of miRNA biogenesis is the precise excision of the small RNA from its precursor. In plants, miRNA precursors are largely variable in size and shape and can be processed by different modes. Here, we optimized an approach to detect intermediates during miRNA biogenesis. We characterized a miRNA whose processing is triggered by a terminal branched loop. Plant miRNA processing can be initiated by internal bubbles, small terminal loops or branched loops followed by dsRNA segments of 15-17 bp. Interestingly, precision and efficiency vary with the processing modes.Despite the various potential structural determinants present in a single a miRNA precursor, DCL1 is mostly guided by a predominant structural region in each precursor in wild-type plants.However, our studies in fiery1, hyl1 and se mutants revealed the existence of cleavage signatures consistent with the recognition of alternative processing determinants. The results provide a general view of the mechanisms underlying the specificity of miRNA biogenesis in plants.

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“…HYL1 contains two dsRNA binding domain at N-terminal followed by nuclear localization signal and these domains are sufficient for its function (Wu et al, 2007). HYL1 dimerizes through its RNA binding domains and this dimerization is essential for its function (Yang et al, 2014(Yang et al, , 2010. HYL1 binds to the stem region and selects the proper cleavage site on pri-miRNA.…”

Section: Formation and Processing Of Pre-mirnasmentioning

confidence: 99%

Regulation of Plant miRNA Biogenesis

Anushree¹,

Shivaprasad

2017

Proceedings of the Indian National Science Academy

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Micro (mi)RNAs are small regulatory RNA molecules involved in post-transcriptional silencing of their target mRNAs. In plants, these miRNAs target mRNAs coding for transcription factors and key regulators, thus modulating plant development, defence, and metabolic processes. miRNAs mostly originate from MIR genes that are transcribed to long primary (pri)miRNA transcripts by Pol II. These pri-miRNA transcripts fold-back to give rise to specific stem-loop structures that are cleaved by Dicer-like (DCL) proteins to Pre-miRNA intermediates. Pre-miRNAs are precisely processed into mature miRNA/miRNA* duplex of 20-22-nt length by DCL1 with the help of few accessory proteins. It is not known why only one or rarely more than one miRNA results from these long dsRNA precursors. In this review, we discuss recent studies including our unpublished work on structural and sequence determinants of plant miRNAs that distinguish them from their precursor regions. These determinants play a major role in accurate and precise dicing of miRNAs from their precursors, thus contributing to abundance and stability of mature miRNAs.

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Homodimerization of HYL1 ensures the correct selection of cleavage sites in primary miRNA

Cited by 58 publications

References 54 publications

The ‘how’ and ‘where’ of plant microRNAs

The ‘how’ and ‘where’ of plant microRNAs

Efficiency and precision of miRNA biogenesis modes in plants

Regulation of Plant miRNA Biogenesis

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