Homodimeric pregnancy-associated plasma protein-A in normal human placenta of first and third trimester of pregnancy: Biochemical and morphological observations

D’Elia, Piera; Marzioni, Daniela; Castellucci, Mario; Mocci, C; Pala, Andrea Norcini

doi:10.1016/j.placenta.2012.09.003

Cited by 8 publications

(11 citation statements)

References 13 publications

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“…By contrast, the proMBP subunit-specific antibody (5H9, used in combination with HRP-conjugated PAPP-A polyclonal antibody P0042) failed to react with the same media. On the other hand, capacity of the same antibody combination (5H9 and P0042) of reacting with PAPP-A/proMBP, present in pregnancy sera or placenta extracts, has been previously demonstrated [27] and confirmed by its ability of reacting with the WHO 78/610 RP at PAPP-A concentrations as low as 40 mU/L. Taken together, these results indicate that only active dPAPP-A and not PAPP-A/proMBP is secreted in both CSp-CM and CDC-CM.…”

Section: Resultsmentioning

confidence: 99%

“…Appropriate negative controls for autofluorescence and aspecific recognition by secondary antibodies have been performed during all staining protocols. Additionally, positive controls for all primary antibodies have been performed on human trophoblast preparations, as described in [27]. …”

Section: Methodsmentioning

confidence: 99%

“…Affinity chromatography of concentrated CMs through Heparin-Sepharose (CL-6B, GE Healthcare) was performed as previously described [27]. Solutions containing 10 U of PAPP-A were applied to 1 × 6 cm column filled with the solid phase and equilibrated with the same Tris buffer solutions, and they allowed recycling at a flow rate of 25 mL/hour for 1 hour.…”

Section: Methodsmentioning

confidence: 99%

“…In all cases, P0042 polyclonal antibody (HRP—conjugated anti-PAPP-A) was used for detection. The anti-proMBP/anti-PAPP-A antibody combination failed to recognize PAPP-A/proMBP complex in both CMs, whereas it was previously shown to be reactive with the pooled third trimester pregnancy serum-derived WHO 78/610 RP and with first and third trimester pregnancy human placental extracts [27]. …”

Section: Figurementioning

confidence: 99%

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Analysis of Pregnancy-Associated Plasma Protein A Production in Human Adult Cardiac Progenitor Cells

D’Elia

Ionta

Chimenti

et al. 2013

BioMed Research International

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IGF-binding proteins (IGFBPs) and their proteases regulate IGFs bioavailability in multiple tissues. Pregnancy-associated plasma protein A (PAPP-A) is a protease acting by cleaving IGFBP2, 4, and 5, regulating local bioavailability of IGFs. We have previously shown that IGFs and IGFBPs are produced by human adult cardiac progenitor cells (haCPCs) and that IGF-1 exerts paracrine therapeutic effects in cardiac cell therapy with CPCs. Using immunofluorescence and enzyme immunoassays, we firstly report that PAPP-A is produced and secreted in surprisingly high amounts by haCPCs. In particular, the homodimeric, enzymatically active, PAPP-A is secreted in relevant concentrations in haCPC-conditioned media, while the enzymatically inactive PAPPA/proMBP complex is not detectable in the same media. Furthermore, we show that both homodimeric PAPP-A and proMBP can be detected as cell associated, suggesting that the previously described complex formation at the cell surface does not occur easily, thus positively affecting IGF signalling. Therefore, our results strongly support the importance of PAPP-A for the IGFs/IGFBPs/PAPP-A axis in CPCs biology.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Analysis of Pregnancy-Associated Plasma Protein A Production in Human Adult Cardiac Progenitor Cells

D’Elia

Ionta

Chimenti

et al. 2013

BioMed Research International

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“…While this high molecular weight glycoprotein is produced in great amounts by trophoblast and deciduas cells, it is also found in other cells (fibroblasts, osteoblasts, vascular smooth muscle cells, ovarian granulose cells) in different concentrations [1–5]. During pregnancy PAPP-A concentrations increase, and it is detectable in maternal blood from the 28 th day after conception [6].…”

Section: Introductionmentioning

confidence: 99%

Serum pregnancy-associated plasma protein A levels in the first, second and third trimester of pregnancy: relation to newborn anthropometric parameters and maternal tobacco smoking

Chełchowska¹,

Gajewska²,

Mazur³

et al. 2016

aoms

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IntroductionThe purpose of this study was to evaluate the associations of the first, second and third trimester serum pregnancy-associated plasma protein A (PAPP-A) concentrations with neonatal anthropometric parameters. The effect of tobacco smoking during pregnancy on PAPP-A level was also studied.Material and methodsOne hundred and fifty healthy pregnant women were divided into smoking and tobacco-abstinent groups. Serum PAPP-A level was measured with the KRYPTOR rapid random-access immunoassay analyzer. The relationship between PAPP-A and newborn related outcome as well as markers of estimated intensity of cigarette smoking was evaluated by univariate and multivariate linear regression.ResultsPregnancy-associated plasma protein A concentration was positively correlated with birth weight in the first (β = 31.6; p < 0.001), second (β = 10.6; p < 0.05), and third (β = 4.6; p < 0.001) trimester of gestation. A significant association between PAPP-A and birth body length and head circumference in the second (β = 0.02; p < 0.05) and third trimester (β = 0.01; p < 0.01) was also found. The serum PAPP-A levels were significantly lower in the smoking than in the tobacco-abstinent group in each trimester of pregnancy (p < 0.001). The largest impact of the number of cigarettes smoked per day on PAPP-A level was found in the second (β = –1.2; p = 0.004) and third trimester (β = –2.6; p = 0.001).ConclusionsMaternal serum PAPP-A levels during gestation might be significant predictors for birth weight. Increased PAPP-A concentrations in the second and third trimester appeared to also be predictive for newborn body length and head circumference. Smoking alters maternal PAPP-A levels in all trimesters, with the greatest impact related to the number of cigarettes smoked per day.

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Transcriptome profile of the human placenta

Majewska

Lipka

Paukszto

et al. 2017

Funct Integr Genomics

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The human placenta is a particular organ that inseparably binds the mother and the fetus. The proper development and survival of the conceptus relies on the essential interplay between maternal and fetal factors involved in cooperation within the placenta. In our study, high-throughput sequencing (RNA-seq) was applied to analyze the global transcriptome of the human placenta during uncomplicated pregnancies. The RNA-seq was utilized to identify the global pattern of the gene expression in placentas (N = 4) from women in single and twin pregnancies. During analyses, we obtained 228,044 transcripts. More than 91% of them were multi-exon, and among them 134 were potentially unknown protein coding genes. Expression levels (FPKM) were estimated for 38,948 transcriptional active regions, and more than 3000 of genes were expressed with FPKM >20 in each sample. Additionally, all unannotated transcripts with estimated FPKM values were localized on the human genome. Highly covered splice junctions unannotated in the human genome (6497) were identified, and among them 30 were novel. To gain a better understanding of the biological implications, the assembled transcripts were annotated with gene ontology (GO) terms. Single nucleotide variants were predicted for the transcripts assigned to each analyzed GO category. Our results may be useful for establishing a general pattern of the gene expression in the human placenta. Characterizing placental transcriptome, which is crucial for a pregnancy’s outcome, can serve as a basis for identifying the mechanisms underlying physiological pregnancy, as well as may be useful for an early detection of the genomic defects.Electronic supplementary materialThe online version of this article (doi:10.1007/s10142-017-0555-y) contains supplementary material, which is available to authorized users.

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Homodimeric pregnancy-associated plasma protein-A in normal human placenta of first and third trimester of pregnancy: Biochemical and morphological observations

Cited by 8 publications

References 13 publications

Analysis of Pregnancy-Associated Plasma Protein A Production in Human Adult Cardiac Progenitor Cells

Analysis of Pregnancy-Associated Plasma Protein A Production in Human Adult Cardiac Progenitor Cells

Serum pregnancy-associated plasma protein A levels in the first, second and third trimester of pregnancy: relation to newborn anthropometric parameters and maternal tobacco smoking

Transcriptome profile of the human placenta

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