Homo- and Heterocellular Junctions in Cell Cultures: An Electrophysiological and Morphological Study

Hyde, Amy; Blondel, Benigna; Matter, Alex; Cheneval, J.P.; Filloux, B.; Girardier, L

doi:10.1016/s0079-6123(08)63247-1

Cited by 124 publications

(62 citation statements)

References 19 publications

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“…Upon contact with hamster cells, the human fibroblasts were hyperpolarized to a level typical for hamster cells. As it was shown by Hyde et al [13], gap junction formation between the fibroblasts and myoblasts from the cardiac tissue of 2-7 days old rats in the mixed culture caused hyperpolarization of the fibroblasts from -20 to -60 mV. This effect is similar to that observed in our experiments.…”

Section: Resultssupporting

confidence: 93%

Energy transfer via cell‐to‐cell junctions

Потапова

Aslanidi²,

Boitzova

1990

FEBS Letters

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Animal cell cooperation has been studied in a mixed cell culture. Membrane potentials of human embryonic cells and hamster BHK-21 cells were recorded by intracellular microelectrodes. The Na+/K+-ATPase inhibitor ouabain (1 x 10W6 M; 2 h) caused strong depolarisation of the human cells in pure culture. The same treatment reduced only slightly the membrane potential in the hamster cells in pure or mixed cultures, as well as in the human cells in mixed culture. The above data can be explained by effective ion fluxes through heterotypic gap junctions in mixed culture. Thus, in the presence of ouabain the Na+/K+-ATPase of hamster cells creates transmembrane differences of the electrochemical potential of ions not only in the hamster cells, but in the human cells as well.

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Section: Resultssupporting

confidence: 93%

Energy transfer via cell‐to‐cell junctions

Потапова

Aslanidi²,

Boitzova

1990

FEBS Letters

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“…In order to record accurately such a high input impedance, not only must a tight seal be made between the membrane and electrode at the site of puncture, but also extraordinary precautions must be made to reduce stray input capacitances and great care taken in compensating for the unavoidable shunt capacitance between the electrode and the grounded bathing solution. The difficulty, if not impossibility, of achieving a tight seal, perhaps accounts for the very low resting potentials that have been recorded from such cells (47,(54)(55)(56)(57). Indeed, all our attempts to make measurements from single isolated cells have not been satisfactory in spite of using microelectrodes of small tip diameter (resistance, 40-60 M~), and a high power microscopic control of several forms of automatic motion for the final insertion of the microelectrode into the cell.…”

Section: Discussionmentioning

confidence: 99%

“…Muhicellular, sheet-like preparations ot tissue-cultured cardiac muscle suffer from the same drawbacks as their naturally occurring counterparts: problems arising from the complex geometry of fiber interconnections. These preparations have been treated theoretically as a one-dimensional cell (flat ribbon or cylinder [47,48,51,62]) although perhaps, in some cases, the assembled clusters of myoblasts right have been better described as a thick, plane cell or semi-infinite solid. In addition, such tissue-cultured preparations generally were "contaminated" with nonmuscle cells, and, to further complicate matters, the single-electrode bridge technique was frequently used in some of these studies in a way that was open to the kind of criticism referred to above.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, for cardiac muscle values ot R~ ranging from 60 to 500 tlcm have been reported for naturally occurring (2,3,39,46) as well as tissue-cultured preparations (47,48). This large spread is without doubt largely attributable to the varying degree to which the actual geometry of the preparation approached the various simple geometries (cylinder, sheet, etc.)…”

Section: Myoplasmic Resistancementioning

confidence: 94%

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A synthetic strand of cardiac muscle: its passive electrical properties.

Lieberman¹,

Sawanobori²,

Kootsey³

et al. 1975

The Journal of General Physiology

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A B S T R n O T The passive electrical properties of synthetic strands of cardiac muscle, grown in tissue culture, were studied using two intraeeUular microelectrodes: one to inject a rectangular pulse of current and the other to record the resultant displacement of membrane potential at various distances from the current source. In all preparations, the potential displacement, instead of approaching a steady value as would be expected for a cell with constant electrical properties, increased slowly with time throughout the current step. In such circumstances, the specific electrical constants for the membrane and cytoplasm must not be obtained by applying the usual methods, which are based on the analytical solution of the partial differential equation describing a one-dimensional cell with constant electrical properties. A satisfactory fit of the potential waveforms was, however, obtained with numerical solutions of a modified form of this equation in which the membrane resistance increased linearly with time. Best fits of the waveforms from 12 preparations gave the foUowing values for the membrane resistance times unit length, membrane capacitance per unit length, and for the myoplasmic resistance: 1.22 4-0.13 X 10 ~ f~cm, 0.224 4-0.023 # F . c m -x, and 1.37 -4-0.13 X 107 ~crn -t, respectively. The value of membrane capacitance per unit length was close to that obtained from the time constant of the foot of the action potential and was in keeping with the generally satisfactory fit of the recorded waveforms with solutions of the cable equation in which the membrane impedance is that of a single capacitor and resistor in parallel. The area of membrane per unit length and the cross-sectional area of myoplasm at any given length of the preparation were determined from light and composite electron micrographs, and these were used to calculate the foUowing values for the specific electrical membrane resistance, membrane capacitance, and the resistivity of the cytoplasm: 20.5 + 3.0 )< 103 ftcm z, 1.54 4-0.24 #F.cm-", and 180 -4-34 ~cm, respectively. I N T R O D U C T I O NIn cardiac muscle, as in other excitable cells, the passive electrical properties of the cell m e m b r a n e a n d cytoplasm must be d e t e r m i n e d from observations of

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“…Recently, however, Spira (48) described 30-50 A gap junctions in all but the deeper layers of embryonic chick myocardial cells from stage 7 (24 hr) to stage 30 (7 days) . Nexuses were also shown in tissue culture between mammalian cardiac myoblasts (5,49), fibroblasts (5), and smooth muscle cells (50,51) . DeHaan and Hirakow (17) reported that small punctate regions of close membrane apposition formed between isolated heart cells within minutes after they established contact .…”

Section: Ultrastructure Of the Strandmentioning

confidence: 91%

Synthetic Strands of Cardiac Muscle

Purdy

Lieberman

Roggeveen

et al. 1972

The Journal of Cell Biology

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Spontaneously active bundles of cardiac muscle (synthetic strands) were prepared from isolated cells of 11-13-day old embryonic chick hearts which were disaggregated with trypsin . Linear orientation of the cells was obtained by plating them on agar-coated culture dishes in which either grooves were cut in the agar film or a thin line of palladium was deposited over the agar . The influence of cell-to-cell and cell-to-substrate interactions was observed with time lapse cinematography and the formation of the synthetic strand was shown to involve both random and guided cell movements, enlargement of aggregates by accretion and coalescence, and the compact linear arrangement of cells along paths of preferential adhesion. Electron microscope investigations of these strands showed that a dispersed population of heart cells organized into an inner core of muscle cells and an outer sheath of fibroblast-like cells . The muscle cells contained well-developed, but widely spaced myofibrils, a developing sarcoplasmic reticulum associated in part with the myofibrils and in part with the sarcolemma, an abundance of nonmembrane bound ribosomes and glycogen, and a prominent Golgi complex. Numerous specialized contacts were observed between the muscle cells in the strand, e.g., fasciae adherentes, desmosomes, and nexuses . A distinct type of muscle cell characterized by its pale appearance was regularly observed in the strand and was noted to be similar to Purkinje cells described in the adult avian conduction system and in developing chick myocardium . The present findings were compared with other observations of the developing myocardium, in situ, and it was concluded that, by a number of criteria, the muscle cells of the strand were differentiating normally and suitably organized for electrophysiological studies .

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Homo- and Heterocellular Junctions in Cell Cultures: An Electrophysiological and Morphological Study

Cited by 124 publications

References 19 publications

Energy transfer via cell‐to‐cell junctions

Energy transfer via cell‐to‐cell junctions

A synthetic strand of cardiac muscle: its passive electrical properties.

Synthetic Strands of Cardiac Muscle

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