Homing of intravenously infused embryonic stem cell-derived cells to injured hearts after myocardial infarction

Jiao, Min; Huang, Xuling; Xiang, Meixiang; Meissner, A.; Chen, Yu; Ke, Qingen; Kaplan, Elina; Rana, Jamal S.; Oettgen, Peter; Morgan, James P.

doi:10.1016/j.jtcvs.2005.12.022

Cited by 38 publications

(20 citation statements)

References 29 publications

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“…Beeres et al [58] showed 6% improvement in ejection fraction after 6 months of treatment, while in a study by Briguori et al [59] there was no difference in ejection fraction after 1-year follow-up. Recently, noncontrolled pilot studies showed comparable results with 4-6% augmentation of ejection fraction at 3 months [41] or 1 year [42], respectively.…”

Section: Clinical Trials In Chronic Left Ventricular Systolic Dysfuncmentioning

confidence: 90%

“…ESCs have been studied extensively in animals. Murine and sheep models of myocardial infarction have been studied, transplanting ESCs, and in short-term follow-up demonstrating improved ejection fraction and vascularity [41,42]. Problems with ESCs, apart from the ethical concerns, include tumorigenicity, difficulty in purification, and host immune response [9].…”

Section: Pathophysiology and Preclinical Researchmentioning

confidence: 99%

See 1 more Smart Citation

Cellular transplantation: future therapeutic options

Allan

Kass

Glover

et al. 2007

Current Opinion in Cardiology

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Section: Clinical Trials In Chronic Left Ventricular Systolic Dysfuncmentioning

confidence: 90%

Section: Pathophysiology and Preclinical Researchmentioning

confidence: 99%

Cellular transplantation: future therapeutic options

Allan

Kass

Glover

et al. 2007

Current Opinion in Cardiology

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“…This possibility received experimental support when ESC-derived cardiomyocytes injected into the tail vein had been shown to translocate to ischemic areas of the heart [70]. Furthermore, an in vitro study observed enhanced ESC-derived cardiomyocytes migration toward cells that over-express TNF (tumor necrosis factor), a cytokine likely to be present at the site of injury [70].…”

Section: Embryonic Stem Cells (Esc)mentioning

confidence: 99%

Adhesion Proteins, Stem Cells, and Arrhythmogenesis

Gillum

Sarvazyan

2008

Cardiovasc Toxicol

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Cell-transplantation therapy is a promising treatment option that is being actively explored as a way to repair cardiac muscle. The ultimate goal is to reconstitute the architecture of the cardiac muscle and to reestablish electrical propagation, while avoiding hypertrophy and scar formation. In this review, we focus on recent advances in the field as well as the difficulties encountered when the engraftment of cells into the host tissue is to be confirmed and functionally characterized. This is critical since incomplete or partial engraftment of transplanted cells within the host cardiac network exacerbates the heterogeneity already present in the injured myocardium and increases its propensity to arrhythmia. We conclude with a brief discussion of how the modulation of cell adhesion via modification of coupling proteins within transplanted cells may facilitate engraftment and alleviate the arrhythmogenic potential of cardiac grafts.

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“…Stringent requirements for purity are particularly demanded for in vivo transplantation studies, where even a low percentage of contaminating MEFs in a large (millions) batch preparation can translate into a substantial number. This is problematic for the intravascular delivery of ES cells, which is an active front of research in tissue repair and regeneration [14,15]. Because the diameter of MEFs is greater than that of a typical capillary, they have a high likelihood of causing embolism in the capillary bed.…”

Section: Introductionmentioning

confidence: 99%

Rapid Single-Step Separation of Pluripotent Mouse Embryonic Stem Cells from Mouse Feeder Fibroblasts

Barron

Lough

et al. 2008

Stem Cells and Development

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Highly enriched, pure populations of pluripotent mouse embryonic stem (mES) cells are a prerequisite to downstream experimental manipulations. However, the existing preplating method does not allow complete removal of co-cultured mouse embryonic fibroblast (MEF) feeder cells. The primary objective of the current investigation was to develop and validate a rapid, single-step separation technique for the complete removal of MEF feeder cells from mES cells. A discontinuous density gradient was prepared using Histopaque 1119 at incremental percentages from the top to bottom of a test tube (20, 40, 60, and 100% in culture medium). A suspension of mES cells and MEF feeder cells was layered on top of the gradient. After centrifugation at 400 x g, ES cells and MEF feeder cells were segregated discretely in separate layers at the 40/20% and 100/60% density interfaces, respectively. The mES cells were enriched to a purity of greater than 99% with a recovery rate of greater than 90%. The separation did not alter the viability or the differentiation potential of mES cells. This study validates a simple technique that enables the preparation of highly enriched mES cells that are essentially free of contaminating MEF feeder cells. The discontinuous density gradient separation method is inexpensive, efficient, rapid, and reproducible. The method can be readily scaled-up to accommodate large batch preparations, enabling a broad range of processing needs. Overall, this simple technique significantly expedites the recovery and enrichment of mES cells from MEFs.

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Homing of intravenously infused embryonic stem cell-derived cells to injured hearts after myocardial infarction

Cited by 38 publications

References 29 publications

Cellular transplantation: future therapeutic options

Cellular transplantation: future therapeutic options

Adhesion Proteins, Stem Cells, and Arrhythmogenesis

Rapid Single-Step Separation of Pluripotent Mouse Embryonic Stem Cells from Mouse Feeder Fibroblasts

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