Homeostatic Regulation of Intestinal Villous Epithelia by B Lymphocytes

Nishiyama, Yasuhiro; Hamada, Hiromasa; Nonaka, Satoshi; Yamamoto, Hiroshi; Nanno, Masanobu; Katayama, Yasuo; Takahashi, Hidemi; Ishikawa, Hiromichi

doi:10.4049/jimmunol.168.8.4241-a

Cited by 5 publications

(7 citation statements)

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“…Immunohistochemical staining was performed essentially as described (Nishiyama et al, 2002). Pregnant mice at 18.5 days postcopulation (dpc) were injected intraperitoneally with 50 mg/g BrdU (Sigma) diluted with PBS.…”

Section: Histopathology and Immunohistochemistrymentioning

confidence: 99%

“…At 1 h after the injection, fetal heads were dissected, fixed, and embedded in paraffin as described above. Immunohistochemical staining was performed essentially as described (Nishiyama et al, 2002). Numbers of BrdUpositive cells in the lens epithelial cells were counted in three wildtype and three HSF4-null embryos.…”

Section: Histopathology and Immunohistochemistrymentioning

confidence: 99%

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HSF4 is required for normal cell growth and differentiation during mouse lens development

Fujimoto

Izu

Seki

et al. 2004

EMBO J

211

290

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The heat shock transcription factor (HSF) family consists of three members in mammals and regulates expression of heat shock genes via a heat shock element. HSF1 and HSF2 are required for some developmental processes, but it is unclear how they regulate these processes. To elucidate the mechanisms of developmental regulation by HSFs, we generated mice in which the HSF4 gene is mutated. HSF4-null mice had cataract with abnormal lens fiber cells containing inclusion-like structures, probably due to decreased expression of gamma-crystallin, which maintains protein stability. Furthermore, we found increased proliferation and premature differentiation of the mutant lens epithelial cells, which is associated with increased expression of growth factors, FGF-1, FGF-4, and FGF-7. Unexpectedly, HSF1 competed with HSF4 for the expression of FGFs not only in the lens but also in other tissues. These findings reveal the lens-specific role of HSF4, which activates gamma-crystallin genes, and also indicate that HSF1 and HSF4 are involved in regulating expression of growth factor genes, which are essential for cell growth and differentiation.

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Section: Histopathology and Immunohistochemistrymentioning

confidence: 99%

Section: Histopathology and Immunohistochemistrymentioning

confidence: 99%

HSF4 is required for normal cell growth and differentiation during mouse lens development

Fujimoto

Izu

Seki

et al. 2004

EMBO J

211

290

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“…Intestinal and hepatic mononuclear cells were isolated according to the methods described previously [28,18]. In brief, the small intestine was removed and Peyer's patches were excised from the intestine.…”

Section: Cell Preparation Antibodies Flow Cytometry and Cell Sortingmentioning

confidence: 99%

Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects

Ueha

Murai

Yoneyama

et al. 2006

Journal of Leukocyte Biology

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Coincidence of the beneficial graft-vs.-tumor (GVT) effects and the detrimental graft-vs.-host disease (GVHD) remains the major obstacle against the widespread use of allogeneic bone marrow transplantation (BMT) as tumor immunotherapy. We here demonstrate that intervention of MAdCAM-1 (mucosal vascular addressin cell adhesion molecule-1) or fractalkine/CX3CL1 after the expansion of allo-reactive donor CD8 T cells selectively inhibits the recruitment of effector donor CD8 T cells to the intestine and alleviates the graft-vs.-host reaction (GVHR) associated intestinal injury without impairing GVT effects. In a nonirradiated acute GVHD model, donor CD8 T cells up-regulate the expression of intestinal homing receptor alpha4beta7 and chemokine receptors CXCR6 and CX3CR1, as they differentiate into effector cells and subsequently infiltrate into the intestine. Administration of anti-MAdCAM-1 antibody or anti-fractalkine antibody, even after the expansion of alloreactive donor CD8 T cells, selectively reduced the intestine-infiltrating donor CD8 T cells and the intestinal crypt cell apoptosis without affecting the induction of donor derived anti-host CTL or the infiltration of donor CD8 T cells in the hepatic tumor. Moreover, in a clinically relevant GVHD model with myeloablative conditioning, these antibodies significantly improved the survival and loss of weight without impairing the beneficial GVT effects. Thus, interruption of alpha4beta7-MAdCAM-1 or CX3CR1-fractalkine interactions in the late phase of GVHD would be a novel therapeutic approach for the separation of GVT effects from GVHR-associated intestinal injury.

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“…Boismenu and Havran (16) have shown that the keratinocyte growth factor produced by activated ␥␦T cells stimulates epithelial cell growth. Very recently, homeostasis of IECs was also found to be regulated by B lymphocytes (17,18). Nishiyama et al (18) reported that the homeostasis of IECs is negatively regulated by B lymphocytes, whereas it is positively regulated by ␥␦T cells (15).…”

mentioning

confidence: 99%

“…Very recently, homeostasis of IECs was also found to be regulated by B lymphocytes (17,18). Nishiyama et al (18) reported that the homeostasis of IECs is negatively regulated by B lymphocytes, whereas it is positively regulated by ␥␦T cells (15). Moreover, it was suggested that cellular interactions among ␥␦IELs, ␣␤IELs, and IECs occur via cell surface molecules, suggesting the existence of an intranet to prevent potential inflammatory responses at the intestinal mucosal surface (19).…”

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confidence: 99%

Role of γδT Cells in the Inflammatory Response of Experimental Colitis Mice

Tsuchiya

Fukuda

Hamada

et al. 2003

The Journal of Immunology

Self Cite

104

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We examined the severity of experimental colitis induced by dextran sulfate sodium (DSS) using immunologically manipulated mice. C57BL/6 mice showed more severe colitis than BALB/c mice, but mice of both strains recovered fully from the disease after the removal of DSS from their drinking water. The infiltrated cells at the lesions were mainly granulocytes in normal littermates. However, C.B-17 scid, IL-7Rα deficient, and TCR-Cβδ double-deficient mice showed severe colitis and did not recover from the disease even after the removal of DSS. It was found that the infiltrated cells at the lesions in the lethal strains were monocytes. Although both TCR-Cδ−/− and TCR-Cβ−/− mice showed severe colitis phenotypes, infiltration in the former is monocyte-dominant while that in the latter is granulocyte-dominant. Thus the type of cells that infiltrate at the lesions of DSS-induced experimental colitis may be controlled by functional T cell subsets. Immunohistological and RT-PCR analyses of the inflamed colon revealed that the murine homologue of human GROα released by some cells under the control of γδT cells is a possible candidate determining the severity of DSS-induced experimental colitis.

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Homeostatic Regulation of Intestinal Villous Epithelia by B Lymphocytes

Cited by 5 publications

References 0 publications

HSF4 is required for normal cell growth and differentiation during mouse lens development

HSF4 is required for normal cell growth and differentiation during mouse lens development

Intervention of MAdCAM-1 or fractalkine alleviates graft-versus-host reaction associated intestinal injury while preserving graft-versus-tumor effects

Role of γδT Cells in the Inflammatory Response of Experimental Colitis Mice

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