Homeostatic regulation of flavonoid and lignin biosynthesis in phenylpropanoid pathway of transgenic tobacco

Shi, Jinhua; Xu, Yan; Sun, Tingting; Shen, Yuxiao; Shi, Qi Xian; Wang, Wenen; Bao, Manzhu; Luo, Hong; Fuzhao, Nian; Ning, Guogui

doi:10.1016/j.gene.2021.146017

Cited by 22 publications

(13 citation statements)

References 49 publications

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“…In this study, a key enzyme involved in lignin synthesis, CCR ( LOC110107374 ), in the turquoise module was identified, suggesting that lignin plays a key regulatory role in the synthesis of flavonoids. This has been confirmed in Arabidopsis , tobacco, peach and other plants ( 34 – 36 ).…”

Section: Discussionmentioning

confidence: 54%

Analysis of the different growth years accumulation of flavonoids in Dendrobium moniliforme (L.) Sw. by the integration of metabolomic and transcriptomic approaches

et al. 2022

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Dendrobium moniliforme (L.) Sw. is a valuable herbal crop, and flavonoids are primarily distributed as active ingredients in the stem, but the composition and synthesis mechanisms of flavonoids in different growth years are not clear. The accumulation of flavonoids in D. moniliforme from four different years was investigated, using a combined metabolomics and transcriptomics approach in this study. The phenylpropanoid and flavonoid biosynthetic pathways were significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs). The widely targeted metabolomics technique revealed a total of 173 kinds of flavonoid metabolites. The metabolomics data confirmed the trend of total flavonoids (TF) content in stems of D. moniliforme, with chalcone, naringenin, eriodictyol, dihydroquercetin, and other flavonoids considerably up-accumulating in the third year. Twenty DEGs were detected that regulate flavonoid synthesis and the expression of these genes in different growth years was verified using real-time quantitative PCR (qRT-PCR). Furthermore, a comprehensive regulatory network was built for flavonoid biosynthesis and it was discovered that there is one FLS gene, one CCR gene and two MYB transcription factors (TFs) with a high connection with flavonoid biosynthesis by weighted gene co-expression network analysis (WGCNA). In this study, the correlation between genes involved in flavonoid biosynthesis and metabolites was revealed, and a new regulatory mechanism related to flavonoid biosynthesis in D. moniliforme was proposed. These results provide an important reference for the farmers involved in the cultivation of D. moniliforme.

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Section: Discussionmentioning

confidence: 54%

Analysis of the different growth years accumulation of flavonoids in Dendrobium moniliforme (L.) Sw. by the integration of metabolomic and transcriptomic approaches

et al. 2022

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“…Post hoc tests with the LSD method between the main effects of different treatments, as shown in Tab 2, showed a significant difference in the flavonoid content in needles between FS and the control, as well as an equivalent significant difference between FS and LCC, but no significant difference was observed between the control and LCC. (Shi, et al 2022), which in turn generates p-coumaroyl CoA, followed by naringenin chalcone synthesis catalyzed by chalcone synthase (Liu, et al 2021c), the precursor of most different accumulated flavonoids in this study. This process was upregulated by caterpillars, but there was no difference in expression between treatments at 8 h. However, as shown in Fig 6, naringenin, a downstream substance of chalcone, was not induced by FS but was significantly higher in FS than in LCC at 8 h. Therefore, mechanism damage can promote naringin depletion and become more intense over time.…”

Section: Content Of Total Flavonoidmentioning

confidence: 64%

Plant hormones and metabolites related to the resistance response to feeding stimulation and leaf clipping control in Chinese pine (Pinus tabuliformis Carr.)

Zhao

Zhou

Tianhua

et al. 2022

Preprint

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To define changes in metabolic pathways in response to mandibulate insect feeding and to provide a reference for further investigation of the molecular mechanisms underlying the development of conifer resistance, Chinese pine ( Pinus tabuliformis Carr.) in good growth status in Huangtuliangzi Forest Farm Pingquan city, Chengde city, Hebei Province, was stimulated by 10 pine caterpillars ( Dendrolimus tabulaefomis Tsai et Liu) as feeding stimulation (FS) and leaf clipping control (LCC) as mechanical damage. The metabolome and total flavonoid content were measured in the needles at 0 (with no treatment, CK), 2, and 8 h after treatment. Plant hormones were measured with needles at 0, 0.5, 1, 1.5, 2, 4, 6, and 8 h after different treatments. The plant hormones jasmonic acid (JA) and auxin (IAA) were upregulated within a short time (2 h) after treatment, showing an expression trend consistent with the corresponding substances in the metabolome. A total of 30.8% of flavonoids were identified by metabolomics analysis. Analysis of KEGG metabolic pathways for the different accumulated metabolites (DAMs) showed that the top pathway annotated and enriched for DAMs in comparison to 2 h after FS (marked as 2 h) and 2 h after LCC (marked as LCC 2 h) was flavonoid biosynthesis; the top pathway mainly annotated and enriched for DAMs in comparison to 8 h and LCC 8 h was the same. Thus, the flavonoid pathway is one of the main pathways involved in resistance formation in conifers, and JA and IAA are involved in the development of resistance and growth during biotic stress.

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“…In transgenic Arabidopsis, mutations in CCR1, CAD1, CAD2 resulted in reduced lignin in stems and anthers, and higher levels of avonol glycosides, sinapoyl malate, and feruloyl malate (Thévenin et al, 2011). In Nicotiana tabacum, ectopic expression of AtCPC not only reduced the content of avonoids, but also upregulated the expression of lignin biosynthesis genes, which in turn signi cantly enhanced lignin accumulation (Shi et al, 2022). As shown in Fig.…”

Section: 4mentioning

confidence: 85%

Transcriptomic and physiological analysis reveal phytohormone and phenylpropanoid biosynthesis in root of Cynanchum auriculatum

et al. 2023

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Baishouwu (Cynanchum auriculatum), a medicinal and food dual-use plant, has been cultivated for centuries and is favored by consumers. C. auriculatum tuberous roots contain large amounts of avonoids, lignin, and other nutrients. However, the developmental characteristics and phenylpropanoid metabolic mechanism in C. auriculatum have not been clari ed. Here, C. auriculatum tuberous roots were observed in three developmental stages, compared with root forming stage (S1), there were signi cant morphological differences in root expanding stage (S2) and harvest stage (S3). Through Illumina HiSeq2500, nine transcriptomic libraries were constructed for transcriptomic analysis. 28,926 DEGs were activated during the development of C. auriculatum tuberous root, and many DEGs were enriched in 'phytohormone signal transduction' and 'phenylpropanoid biosynthesis'. The analysis of phytohormone content and gene expression revealed that, auxin, cytokinin, and ethylene participated in the regulation of C. auriculatum tuberous root development. With phloroglucinol staining, it was observed that ligni ed cells were mainly distributed in the central xylem at S1, followed by ring-like structure formation in S2, and nally formed the connecting rays between the xylem and the phloem in S3. Lignin content increased at S2 and then decreased at S3, and the expression of lignin synthesis genes also presented a similar trend. Total avonoids content showed a gradually increasing trend, and the expression of avonoid synthesis genes was also gradually up regulated. C. auriculatum might divert the precursors to the avonoid synthesis pathway by reducing the activity of key enzymes in lignin synthesis, resulting in the reduction of lignin content and the promotion of avonoid synthesis. This study provided a basis for the developmental mechanism of C. auriculatum and the further utilization of C. auriculatum tuberous roots.

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Homeostatic regulation of flavonoid and lignin biosynthesis in phenylpropanoid pathway of transgenic tobacco

Cited by 22 publications

References 49 publications

Analysis of the different growth years accumulation of flavonoids in Dendrobium moniliforme (L.) Sw. by the integration of metabolomic and transcriptomic approaches

Analysis of the different growth years accumulation of flavonoids in Dendrobium moniliforme (L.) Sw. by the integration of metabolomic and transcriptomic approaches

Plant hormones and metabolites related to the resistance response to feeding stimulation and leaf clipping control in Chinese pine (Pinus tabuliformis Carr.)

Transcriptomic and physiological analysis reveal phytohormone and phenylpropanoid biosynthesis in root of Cynanchum auriculatum

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