Homeostasis of plasma membrane viscosity in fluctuating temperatures

Martinière, Alexandre; Shvedunova, Maria; Thomson, Adrian; Evans, Nicola H.; Penfield, Steven; Runions, John; McWatters, Harriet G.

doi:10.1111/j.1469-8137.2011.03821.x

Cited by 78 publications

(59 citation statements)

References 39 publications

(45 reference statements)

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“…With regard to lipids, the amounts of linoleic and linolenic acids showed temperature-related fluctuations in cotton seedlings [89]. It has also been shown that only 18∶1 FA, but not other fatty acids, oscillates under diurnal cycles in Arabidopsis, with higher levels accumulated in the light rather than the dark [90]. Besides FA, phosphatidylcholine was recently reported to oscillate diurnally and affects florigen-mediated flowering [91].…”

Section: Discussionmentioning

confidence: 99%

Gene Expression in Plant Lipid Metabolism in Arabidopsis Seedlings

et al. 2014

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Events in plant lipid metabolism are important during seedling establishment. As it has not been experimentally verified whether lipid metabolism in 2- and 5-day-old Arabidopsis thaliana seedlings is diurnally-controlled, quantitative real-time PCR analysis was used to investigate the expression of target genes in acyl-lipid transfer, β-oxidation and triacylglycerol (TAG) synthesis and hydrolysis in wild-type Arabidopsis WS and Col-0. In both WS and Col-0, ACYL-COA-BINDING PROTEIN3 (ACBP3), DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) and DGAT3 showed diurnal control in 2- and 5-day-old seedlings. Also, COMATOSE (CTS) was diurnally regulated in 2-day-old seedlings and LONG-CHAIN ACYL-COA SYNTHETASE6 (LACS6) in 5-day-old seedlings in both WS and Col-0. Subsequently, the effect of CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) from the core clock system was examined using the cca1lhy mutant and CCA1-overexpressing (CCA1-OX) lines versus wild-type WS and Col-0, respectively. Results revealed differential gene expression in lipid metabolism between 2- and 5-day-old mutant and wild-type WS seedlings, as well as between CCA1-OX and wild-type Col-0. Of the ACBPs, ACBP3 displayed the most significant changes between cca1lhy and WS and between CCA1-OX and Col-0, consistent with previous reports that ACBP3 is greatly affected by light/dark cycling. Evidence of oil body retention in 4- and 5-day-old seedlings of the cca1lhy mutant in comparison to WS indicated the effect of cca1lhy on storage lipid reserve mobilization. Lipid profiling revealed differences in primary lipid metabolism, namely in TAG, fatty acid methyl ester and acyl-CoA contents amongst cca1lhy, CCA1-OX, and wild-type seedlings. Taken together, this study demonstrates that lipid metabolism is subject to diurnal regulation in the early stages of seedling development in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

Gene Expression in Plant Lipid Metabolism in Arabidopsis Seedlings

et al. 2014

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“…FRAP experiments were performed according to Martinière et al (2011b) on infected or healthy Arabidopsi s GFP-TU6 leaves using a Zeiss LSM700 confocal microscope with a 63× NA 1.4 oil-immersion objective. Leaf samples were mounted in 1% low melting point agar to prevent focus shift.…”

Section: Methodsmentioning

confidence: 99%

A virus responds instantly to the presence of the vector on the host and forms transmission morphs

Martinière¹,

Bak²,

Macia³

et al. 2013

eLife

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Many plant and animal viruses are spread by insect vectors. Cauliflower mosaic virus (CaMV) is aphid-transmitted, with the virus being taken up from specialized transmission bodies (TB) formed within infected plant cells. However, the precise events during TB-mediated virus acquisition by aphids are unknown. Here, we show that TBs react instantly to the presence of the vector by ultra-rapid and reversible redistribution of their key components onto microtubules throughout the cell. Enhancing or inhibiting this TB reaction pharmacologically or by using a mutant virus enhanced or inhibited transmission, respectively, confirming its requirement for efficient virus-acquisition. Our results suggest that CaMV can perceive aphid vectors, either directly or indirectly by sharing the host perception. This novel concept in virology, where viruses respond directly or via the host to the outside world, opens new research horizons, that is, investigating the impact of ‘perceptive behaviors’ on other steps of the infection cycle.DOI: http://dx.doi.org/10.7554/eLife.00183.001

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“…A growing number of publications details composition changes in a variety of organisms, for example bacteria [1], yeast [2], plants [3], insects [4], fish [5], hibernating mammals [6] and mammalian cell culture lines [7]. Although the processes through which this control is achieved vary across domains and species, the general consensus is that lipid homeostasis is regulated both transcriptionally and biochemically [1].…”

Section: Introductionmentioning

confidence: 99%

Anin vivoratio control mechanism for phospholipid homeostasis: evidence from lipidomic studies

Dymond

hague

Postle

et al. 2013

J. R. Soc. Interface.

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While it is widely accepted that the lipid composition of eukaryotic membranes is under homeostatic control, the mechanisms through which cells sense lipid composition are still the subject of debate. It has been postulated that membrane curvature elastic energy is the membrane property that is regulated by cells, and that lipid composition is maintained by a ratio control function derived from the concentrations of type II and type 0 lipids, weighted appropriately. We assess this proposal by seeking a signature of ratio control in quantified lipid composition data obtained by electrospray ionization mass spectrometry from over 40 independent asynchronous cell populations. Our approach revealed the existence of a universal 'pivot' lipid, which marks the boundary between type 0 lipids and type II lipids, and which is invariant between different cell types or cells grown under different conditions. The presence of such a pivot species is a distinctive signature of the operation in vivo, in human cell lines, of a control function that is consistent with the hypothesis that membrane elastic energy is homeostatically controlled.

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Homeostasis of plasma membrane viscosity in fluctuating temperatures

Cited by 78 publications

References 39 publications

Gene Expression in Plant Lipid Metabolism in Arabidopsis Seedlings

Gene Expression in Plant Lipid Metabolism in Arabidopsis Seedlings

A virus responds instantly to the presence of the vector on the host and forms transmission morphs

Anin vivoratio control mechanism for phospholipid homeostasis: evidence from lipidomic studies

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