Homeostasis and Defense at the Surface of the Eye. The Conjunctival Microbiota

Garza, Arnulfo; Diaz, Giancarlo; Hamdan, Marah; Shetty, Akaanksh; Hong, Bo-Young; Cervantes, Jorge

doi:10.1080/02713683.2020.1788100

Cited by 16 publications

(9 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 18 – 20 Normal OS has relatively stable, and low diversity colonized microbiota, 21 – 23 which help maintain homeostasis. 24 However, diseases may change the microbiota composition of the OS, negatively affecting the protective role of the microbiota and causing it to be harmful. 21 , 25 – 27 …”

mentioning

confidence: 99%

Ocular Surface Microbiota in Diabetic Patients With Dry Eye Disease

Zhang

Zou²,

Xue

et al. 2021

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

Purpose To investigate the ocular surface (OS) commensal bacteria profiles of patients with diabetes mellitus (DM) and dry eye disease (DED). Methods In the present study, subjects were assigned to four groups: 37 to the diabetic mellitus with dry eye disease (DM with DED) group, 22 to the diabetes mellitus (DM)-only group, 34 to the dry eye disease (DED)-only group, and 22 to the control group. Tear fluid was collected using Schirmer's tear secretion test paper. 16S ribosomal ribonucleic acid (rRNA) gene sequencing was used to analyze the bacterial microbiota. Results The DM with DED group showed the highest operational taxonomic unit (OTU) numbers and alpha diversity and the most different beta diversity. The groups shared the four most abundant phyla, accounting for over 96% of the total abundance. At the genus level, there were 10 types of overlap in the core microbiota in the groups. They showed significant differences between the groups. Additionally, the DM with DED group and the control group showed four unique core genera, respectively. Unclassified Clostridiales and Lactobacillus were the core microbiota members of the DM with DED group, the DM-only group, and the DED-only group, but not the control group. Conclusions In the present study, our results showed that the patients in the DM with DED group had a more complex and comprehensive ocular surface microbial composition. To the best of our knowledge, this is the first study to reveal the microbial profile of dry eye disease in patients with diabetes mellitus.

show abstract

mentioning

confidence: 99%

Ocular Surface Microbiota in Diabetic Patients With Dry Eye Disease

Zhang

Zou²,

Xue

et al. 2021

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…13 The core microbiome of the conjunctiva is composed of the genera; Corynebacterium, Acinetobacter, Pseudomonas, Staphylococcus, Streptococcus, Bacillus, Anerococcus, Millisia, and Finegoldia. 14 The metagenomic study of primary acquired nasolacrimal duct obstruction (PANDO) has demonstrated the prevalent organisms to include Acinetobacter johnsonii, Porphyromonas catoniae, Cutibacterium acnes, Pseudomonas alcaliphila, Escherichia coli, and Hemophilus influenzae, with an alpha diversity range of 60 to 175 species. 15 The functional profiling of microbial communities in PANDO has demonstrated their involvement in a wide range of metabolic processes, environmental information processes, genetic information processes, cell cycle regulation, dormancy, and sporulation.…”

Section: Discussionmentioning

confidence: 99%

Metagenomics of infective canaliculitis: The Lacriome paper 3

Ali

2022

European Journal of Ophthalmology

View full text Add to dashboard Cite

Purpose To study the metagenomics of the microbes isolated from the canaliculus of patients with infective canaliculitis. Methods A prospective study was performed on five consecutive canalicular samples obtained for the metagenomic analysis from the patients with infective canaliculitis who underwent non-incisional canalicular curettage at a tertiary care Dacryology service. The canalicular concretions were collected intraoperatively soon after a canalicular curettage and immediately transported on ice to the laboratory. Following DNA extraction and library preparation, a whole shotgun metagenome sequencing was performed on the Illumina™ platform. The downstream processing and bioinformatics of the samples were performed using multiple software packaged in SqueezeMeta™ pipeline or MG-RAST™ pipeline. Results The taxonomic hit distribution across the samples showed that bacteria were the most common isolates (mean—80.5%), followed by viruses (mean—0.74%), and archaea (0.01%). The five major phyla identified across the samples of infective canaliculitis were, Fusobacteria, Bacteroidetes, Proteobacteria, Actinobacteria, and Firmicutes. The prevalent organisms include Fusobacterium nucelatum, Fusobacterium periodonticum, Parvimonas micra, Prevotella oris, Selonomonas noxia, Pseudopropionobacterium propoinicum, Campylobacter showae, and Streptococcus anginosus, amongst few others. Actinomycetes israelii was noted in all the samples, though it was not the most abundant. The microbial gene mapping and protein prediction demonstrated proteins with known functions to range from 69.91% to 87.09% across the samples. The functional subsystem profiling demonstrated genes associated with carbohydrate, amino acid, and co-enzyme transport and metabolism, cell wall or cell membrane biogenesis, energy production and conversion, transcription, translation, and cellular communications. Conclusion This is the first whole metagenome sequencing of infective canaliculitis. Infected canaliculi harbor diverse microbial communities, including bacteria, viruses, and archaea. Functional analysis has provided newer insights into the ecosystem dynamics and strategies of microbial communities.

show abstract

“…23 The genera Corynebacterium, Acinetobacter, Pseudomonas, Staphylococcus, and Streptococcus constitute the core conjunctival microbiome. 24 The sino-nasal microbiome is predominantly contributed by the Corynebacterium, Staphylococcus, Streptococcus, Hemophilus , and the Moraxella species. 25,26 The present study showed that the stent microbiome core phyla include Proteobacteria, Firmicutes, and Actinobacteria.…”

Section: Discussionmentioning

confidence: 99%

Microbial Metagenomics of the Extubated Lacrimal Stents Following Dacryocystorhinostomy: The Lacriome Paper 4

Ali

2022

Ophthalmic Plastic &Amp; Reconstructive Surgery

View full text Add to dashboard Cite

Purpose: To study the metagenome of the microbes present on the extubated lacrimal stents following a dacryocystorhinostomy. Methods: A prospective study was performed on 10 consecutive extubated lacrimal stents obtained for the metagenomic analysis from the patients following an endoscopic dacryocystorhinostomy. The stents were extubated at 4 weeks postoperatively under endoscopic guidance and immediately transported on ice to the laboratory. Following DNA extraction and library preparation, a whole shotgun metagenome sequencing was performed on the Illumina platform. The downstream processing and bioinformatics of the samples were performed using multiple software packaged in SqueezeMeta pipeline or MG-RAST pipeline. Results: The taxonomic hit distribution across the stent samples showed that bacteria were the most common isolates (mean, 69.70%), followed by viruses (mean, 0.02%) and archaea (0.003%). The 3 major phyla identified were Firmicutes, Actinobacteria, and Proteobacteria. The prevalent organisms include Pseudomonas aeruginosa, Staphylococcus aureus, Corynebacterium accolens, Dolosigranulum pigrum, Citrobacter koserii, Staphylococcus epidermidis, E. coli, and Hemophilus influenza. The functional subsystem profiling demonstrated microbial genes associated with metabolism, cellular, and information processing. The functional subsystem categories were metabolism involving carbohydrates, amino acids, DNA and RNA, cell wall or cell capsule biogenesis, membrane transport, virulence, and defense mechanisms. Conclusions: The present study is the first whole metagenome sequencing of the microbes isolated from the extubated lacrimal stents. The stents harbor diverse microbial communities with distinct ecosystem dynamics. Further studies on microbes-host interactions in the early postoperative period would provide valuable insights.

show abstract

Homeostasis and Defense at the Surface of the Eye. The Conjunctival Microbiota

Cited by 16 publications

References 52 publications

Ocular Surface Microbiota in Diabetic Patients With Dry Eye Disease

Ocular Surface Microbiota in Diabetic Patients With Dry Eye Disease

Metagenomics of infective canaliculitis: The Lacriome paper 3

Microbial Metagenomics of the Extubated Lacrimal Stents Following Dacryocystorhinostomy: The Lacriome Paper 4

Contact Info

Product

Resources

About