Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish

Ogawa, Yohsuke; Shiraki, Tomoya; Kojima, Daisuke; Fukada, Yoshitaka

doi:10.1098/rspb.2015.0659

Cited by 42 publications

(113 citation statements)

References 46 publications

(56 reference statements)

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“…Additionally, the fish-specific transcription factor Sine oculis homeobox homolog 7 (Six7) drives activation of RH2 (Fig. 2C, Step 3 ) (191) . In mice, Shh signaling may activate Sall3, which acts with Rorβ to activate S-opsin (Fig.…”

Section: The Gene-regulatory Network Controlling Pr Specification Shmentioning

confidence: 99%

Mechanisms of Photoreceptor Patterning in Vertebrates and Invertebrates

2016

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Across the animal kingdom, visual systems have evolved to be uniquely suited to the environments and behavioral patterns of different species. The visual acuity and color perception of organisms depend on the distribution of photoreceptor subtypes within the retina. Retinal mosaics can be organized into three broad categories: stochastic/regionalized, regionalized, and ordered. Here, we describe the retinal mosaics of flies, zebrafish, chickens, mice, and humans and the gene regulatory networks controlling proper photoreceptor specification in each. By drawing parallels in eye development between these divergent species, we identify a set of conserved organizing principles and transcriptional networks that govern photoreceptor subtype differentiation.

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Section: The Gene-regulatory Network Controlling Pr Specification Shmentioning

confidence: 99%

Mechanisms of Photoreceptor Patterning in Vertebrates and Invertebrates

2016

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“…Teleost fish have the innate capacity to regenerate photoreceptors after loss [7–9]. Teleost fish have contributed much towards the first hurdle described above (regenerating cones, rather than regenerating rods), revealing gene regulatory networks that promote generation of cone photoreceptors; this appears to include interactions amongst tbx2b , gdf6a , six7 and thyroid signaling [10–12]. Experiments in mice have produced a more fulsome understanding of this network, revealing Nrl, Nr2e3 and thyroid receptor β as being critical to cone specification [13, 14].…”

Section: Introductionmentioning

confidence: 99%

Rapid Recovery of Visual Function Associated with Blue Cone Ablation in Zebrafish

et al. 2016

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Hurdles in the treatment of retinal degeneration include managing the functional rewiring of surviving photoreceptors and integration of any newly added cells into the remaining second-order retinal neurons. Zebrafish are the premier genetic model for such questions, and we present two new transgenic lines allowing us to contrast vision loss and recovery following conditional ablation of specific cone types: UV or blue cones. The ablation of each cone type proved to be thorough (killing 80% of cells in each intended cone class), specific, and cell-autonomous. We assessed the loss and recovery of vision in larvae via the optomotor behavioural response (OMR). This visually mediated behaviour decreased to about 5% or 20% of control levels following ablation of UV or blue cones, respectively (P<0.05). We further assessed ocular photoreception by measuring the effects of UV light on body pigmentation, and observed that photoreceptor deficits and recovery occurred (p<0.01) with a timeline coincident to the OMR results. This corroborated and extended previous conclusions that UV cones are required photoreceptors for modulating body pigmentation, addressing assumptions that were unavoidable in previous experiments. Functional vision recovery following UV cone ablation was robust, as measured by both assays, returning to control levels within four days. In contrast, robust functional recovery following blue cone ablation was unexpectedly rapid, returning to normal levels within 24 hours after ablation. Ablation of cones led to increased proliferation in the retina, though the rapid recovery of vision following blue cone ablation was demonstrated to not be mediated by blue cone regeneration. Thus rapid visual recovery occurs following ablation of some, but not all, cone subtypes, suggesting an opportunity to contrast and dissect the sources and mechanisms of outer retinal recovery during cone photoreceptor death and regeneration.

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“…For example, RGC specification trajectories confirmed several known differentiation regulators including sox11a, sox11b, sox6, irx4a, and pou4f2 28 . Similarly, known regulators of photoreceptor differentiation, such as isl2a 29 , prdm1a 30 , otx5 31 , and crx 32 are expressed early in our photoreceptor trajectories, while known regulators of cone versus rod fate, such as six7 33 , nr2f1b 34 , and nr2e3 35 are expressed as those trajectories diverge. Furthermore, our analysis revealed novel transcription factors within the gene expression cascades.…”

Section: Cell Specification Trajectories In the Retinamentioning

confidence: 98%

Emergence of neuronal diversity during vertebrate brain development

Raj

Farrell

McKenna

et al. 2019

Preprint

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Neurogenesis in the vertebrate brain comprises many steps ranging from the proliferation of progenitors to the differentiation and maturation of neurons. Although these processes are highly regulated, the landscape of transcriptional changes and progenitor identities underlying brain development are poorly characterized. Here, we describe the first developmental single-cell RNAseq catalog of more than 200,000 zebrafish brain cells encompassing 12 stages from 12 hours post-fertilization to 15 days post-fertilization. We characterize known and novel gene markers for more than 800 clusters across these timepoints. Our results capture the temporal dynamics of multiple neurogenic waves from embryo to larva that expand neuronal diversity from ~20 cell types at 12 hpf to ~100 cell types at 15 dpf. We find that most embryonic neural progenitor states are transient and transcriptionally distinct from long-lasting neural progenitors of post-embryonic stages. Furthermore, we reconstruct cell specification trajectories for the retina and hypothalamus, and identify gene expression cascades and novel markers. Our analysis reveal that late-stage retinal neural progenitors transcriptionally overlap cell states observed in the embryo, while hypothalamic neural progenitors become progressively distinct with developmental time. These data provide the first comprehensive single-cell transcriptomic time course for vertebrate brain development and suggest distinct neurogenic regulatory paradigms between different stages and tissues.

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Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish

Cited by 42 publications

References 46 publications

Mechanisms of Photoreceptor Patterning in Vertebrates and Invertebrates

Mechanisms of Photoreceptor Patterning in Vertebrates and Invertebrates

Rapid Recovery of Visual Function Associated with Blue Cone Ablation in Zebrafish

Emergence of neuronal diversity during vertebrate brain development

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