Holştayn Sığırlarında Foliküler ve Luteal Fazdaki Ovaryum Dokularında İntraovarian Genlerin Ekspresyon Profili

Abstract

The aim of the present study is to determine comparatively expression levels of the BMP15, TGFB1, TGFB2 and GDF9 genes in the preovulatory follicle and corpus luteum tissue of Holstein cattle. For this purpose, primarily the tissues were examined by immunohistochemical staining. Later on, follicular fluid was analyzed by ELISA test and estradiol and progesterone levels were determined. Finally, expression levels of the related genes were determined between the groups by qRT-PCR. Immunohistochemical staining revealed that estrogen receptor alpha and progesterone receptor immunoreactivities were intensely present in preovulatory follicles, estrogen alpha receptor immunoreactivity was very slight and progesterone receptors were similar to positivity in preovulatory follicles in corpus luteum. Furthermore, estradiol level was high in preovulatory follicles and progesterone level was high in corpus luteum. The levels of mRNA transcripts of the TGFB1 and TGFB2 genes in the preovulatory follicles were statistically higher than the corpus luteum (p<0.01, p<0.05, respectively), but there was no statistically significant difference between the groups in the mRNA transcript levels of the BMP15 and GDF9 genes (p>0.05). As a result, it is thought that differential expression of intraovarian genes may be associated with differences in follicular dynamics and gene expression levels within the cell population of ovarian tissue, so this situation may result in follicular and luteal phase.