HoloVision: a software package for reconstruction and analysis of digitally sampled holograms

Skotheim, Øystein

doi:10.1117/12.516656

Cited by 7 publications

(6 citation statements)

References 2 publications

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“…named A, D, and MSD. Digital reconstruction of the recorded interference patterns is performed using the "HoloVision 2.2" software [6]. Figures 2, 3 and 4 present the holograms and the reconstructed intensities to represent the objects.…”

Section: Resultsmentioning

confidence: 99%

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Digital Holographic Microscopy for Characterization of <i>Fabiana Imbricata Ruiz & Pav.</i> Cell Suspension Cultures

et al. 2019

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An application of digital holographic microscopy (DHM) for measuring the size of cell clusters in cell suspension cultures in vitro is reported for the first time. Undifferentiated plant cell cultures are widely used for fundamental and applied research, for the elucidation of biosynthetic pathways, production of secondary metabolites and as screening tools for biotechnology in pharmacy, food technology, and agriculture. Digital in-line holographic microscopy has been applied to visualize three different cell suspensions of Fabiana imbricata Ruiz & Pav., named A, D, and MSD. Digital reconstruction of the recorded interference patterns was performed using an appropriate software. The reconstructed intensities represent the cell clusters in the suspensions under observation. Cell suspension cultures of Fabiana imbricata Ruiz & Pav. consists of cell aggregates dispersed and growing in shaking liquid media. Small cell aggregates with dimensions between 120 and 180 µm have been observed in all suspensions. The measurement of growth parameters in different cultures introduces diverse problems that must be addressed by using a specific methodology for each type of callus and cell suspension cultures. The attractive features of digital holographic microscopy are noncontact, nondestructive, marker-free in vivo imaging and quantifying biological cells and tissues. It is an advantageous technique for application in biological research and the agricultural sciences. It is shown that DIHM is a new microscopic technique easy to apply to study the size and the structure of cell aggregates in cell suspension cultures in vitro.

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Section: Resultsmentioning

confidence: 99%

“…After the pinhole, the spherical wave passes through the object: the diffracted by the object and the nondiffracted wave interfere as a hologram, which is recorded on a CCD sensor. The intensity and the phase are reconstructed numerically [6].…”

Section: Optical Setup Of the Holographic Microscopementioning

confidence: 99%

Digital Holographic Microscopy for Characterization of <i>Fabiana Imbricata Ruiz & Pav.</i> Cell Suspension Cultures

et al. 2019

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“…Phase-shifting is not necessary in Digital Holography, because the phase is a direct result of the numerical evaluation of the diffraction integral [12]. The free software package HoloVision [21] was used to perform the reconstructions.…”

Section: Methodsmentioning

confidence: 99%

Multi-technique platform for dynamic and static MEMS characterisation

et al. 2007

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MEMS characterisation is an important application area for interferometry. In this paper a Mach-Zehnder interferometer configuration is presented that combines both coherent and low coherent techniques in one setup. It incorporates the application of classical Laser Interferometry (LI) and Electronic Speckle Pattern Interferometry as well as classical Low Coherence Interferometry (LCI), full-field Optical Coherence Tomography and Low Coherence Speckle Interferometry. Digital Holography can be applied by minor modifications of the setup. The setup, working principle, and applications of the interferometer will be described. Measurements on a MEMSbased pressure sensor are presented. The sensor consists of a glass wafer attached to a silicon membrane. A cavity is etched into the glass wafer. The wafers are bonded and form a vacuum cavity. Membrane deformations are measured through the window using LI and LCI. LCI provides information about the shape of the glass window. Results from speckle techniques are compared with similar results from plane wave techniques. The influence of the glass window and the illumination of the object are investigated.

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Section: Optical Setup Of the Digital Holographic Microscopementioning

confidence: 99%

Recent Advances in the Application of Digital Holographic Microscopy

Mihaylova

2019

Acta Phys. Pol. A

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Holography is a technique by which a wavefront can be recorded and subsequently reconstructed in the absence of the original wavefront. In conventional holography, invented by Gabor, the holograms are photographically recorded and optically reconstructed. Digital holography is an emerging new field in imaging applications, mainly in microscopy. By replacing the photochemical procedures with electronic recording of a hologram and its digital reconstruction, a wide range of new imaging capabilities become available. Once the amplitude and the phase of the light wave are recorded numerically, one can easily subject these data to a variety of manipulations, and so digital holography offers capabilities not available in conventional holography. An increasing number of researchers are realizing and exploiting the remarkable capabilities of digital holography. A brief review of the research carried out at the Agricultural University of Plovdiv in the area of digital holographic microscopy is presented. Recent applications of DIHM in materials studies are reported, in particular studies of polymer-zeolite nanocomposites.

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HoloVision: a software package for reconstruction and analysis of digitally sampled holograms

Cited by 7 publications

References 2 publications

Digital Holographic Microscopy for Characterization of <i>Fabiana Imbricata Ruiz & Pav.</i> Cell Suspension Cultures

Digital Holographic Microscopy for Characterization of <i>Fabiana Imbricata Ruiz & Pav.</i> Cell Suspension Cultures

Multi-technique platform for dynamic and static MEMS characterisation

Recent Advances in the Application of Digital Holographic Microscopy

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HoloVision: a software package for reconstruction and analysis of digitally sampled holograms

Cited by 7 publications

References 2 publications

Digital Holographic Microscopy for Characterization of <i>Fabiana Imbricata Ruiz &amp; Pav.</i> Cell Suspension Cultures

Digital Holographic Microscopy for Characterization of <i>Fabiana Imbricata Ruiz &amp; Pav.</i> Cell Suspension Cultures

Multi-technique platform for dynamic and static MEMS characterisation

Recent Advances in the Application of Digital Holographic Microscopy

Contact Info

Product

Resources

About

Digital Holographic Microscopy for Characterization of <i>Fabiana Imbricata Ruiz & Pav.</i> Cell Suspension Cultures

Digital Holographic Microscopy for Characterization of <i>Fabiana Imbricata Ruiz & Pav.</i> Cell Suspension Cultures