Holography: The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

El-Schich, Zahra; Kamlund, Sofia; Janicke, Birgit; Alm, Kersti; Wingren, Anette Gjörloff

doi:10.5772/66042

Cited by 9 publications

(3 citation statements)

References 53 publications

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“…DHC applications have previously been used to discriminate between the maturity levels of red blood cells, perform cell death studies and analyze drug responses [23,[37][38][39]. Cell optical area, thickness and volume are useful parameters, particularly for discrimination between different cell types [39][40][41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

El-Schich

Janicke²,

Alm³

et al. 2020

Applied Sciences

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Breast cancer is the second most common cancer worldwide. Metastasis is the main reason for death in breast cancer, and today, there is a lack of methods to detect and isolate circulating tumor cells (CTCs), mainly due to their heterogeneity and rarity. There are some systems that are designed to detect rare epithelial cancer cells in whole blood based on the most common marker used today, the epithelial cell adhesion molecule (EpCAM). It has been shown that aggressive breast cancer metastases are of non-epithelial origin and are therefore not always detected using EpCAM as a marker. In the present study, we used an in vitro-based circulating tumor cell model comprising a collection of six breast cancer cell lines and white blood cell lines. We used digital holographic cytometry (DHC) to characterize and distinguish between the different cell types by area, volume and thickness. Here, we present significant differences in cell size-related parameters observed when comparing white blood cells and breast cancer cells by using DHC. In conclusion, DHC can be a powerful diagnostic tool for the characterization of CTCs in the blood.

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Section: Discussionmentioning

confidence: 99%

Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

El-Schich

Janicke²,

Alm³

et al. 2020

Applied Sciences

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“…DHM with quantitative phase imaging (QPI) allows observation and quantitative phase imaging of microscopic materials without any pretreatments, such as staining and fixation. DHM has been widely used in medical fields to image live cells because of its nondestructive nature, which allows real-time observations without harming the cells. − In diatom research, Memmolo et al performed a thorough analysis of the classification of diatoms using holographic microscopy with machine learning . Moreover, observation of unseparated diatom frustule via DHM was reported by Saito et al…”

Section: Introductionmentioning

confidence: 99%

Imaging and Quantitative Analysis on the Etching of Diatom Frustules via Digital Holographic Microscopy

Lugo,

Saito,

Kitamura

et al. 2023

ACS Biomater. Sci. Eng.

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Frustules, whose length spans from a few micrometers to more than a hundred micrometers, have been the subject of various modifications to improve their physical properties because of their complex porous silica structure. However, threedimensional measurements of these changes can be challenging because of the complex 3D architecture and limitations of known methods. In this study, we present a new method that applies digital holographic microscopy (DHM) to analyze controlled etched frustules and observe real-time degradation of frustules at the single-cell level. Frustules obtained from Craspedostauros sp. diatoms were etched in 1 N NaOH for 5 min at 25 and 60 °C, respectively, and the frustule's valve was analyzed using DHM. DHM uses a combination of holography and tomography to reconstruct a 3D refractive index image of the frustule. Measurements of the width, volume, and surface area are achieved. Results showed that at 60 °C of etching, a significant difference with the unetched frustule was observed for all measurements but with high fluctuation values. Finally, real-time observation of the degradation of the frustule is observed when immersed in a high concentration of NaOH. This is the first time the real-time etching of the frustule is observed at the single-cell level. This research provides an easy estimation of the 3D measurements of frustules that may provide new fundamental information and applications.

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“…In this way, it is feasible to correlate information between the healthy or pathological states of the cells. DHM also provides 2D and 3D sample data, since the optical phase and amplitude information embedded in the interferometric patterns can be retrieved [3,14,15].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous dual-wavelength digital holographic microscopy as a tool for the analysis of keratoacanthoma skin samples

Palacios-Ortega,

Hernández-Montes,

Santoyo

et al. 2023

J. Phys. D: Appl. Phys.

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A keratoacanthoma skin tumor is usually caused by sun exposure and may be an alert sign prior to the development of a more aggressive tumor or skin cancer. Studying the shape of the keratoacanthoma cells and their 3D rendering visualization are important parameters to prevent its evolution to higher stages of tumor cells or skin cancer. Keratoacanthoma cells shape can be obtained through digital holographic microscopy (DHM); for that purpose, a setup with two illumination wavelengths (532 and 638 nm) is implemented to render a synthetic wavelength of 3.2 μm that avoids wrapping the optical phase of the processed holograms and increases measurement range. To recover the optical phase, two off-axis digital holograms are simultaneously recorded at each wavelength. From the processed hologram height variations, the shape and length of keratoacanthoma cells, as well as the stratum corneum epidermal layer, are obtained as phase images. The results achieved aid to discriminate healthy from malignant cells

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Holography: The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

Cited by 9 publications

References 53 publications

Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

Imaging and Quantitative Analysis on the Etching of Diatom Frustules via Digital Holographic Microscopy

Simultaneous dual-wavelength digital holographic microscopy as a tool for the analysis of keratoacanthoma skin samples

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