Holography and Coherent Diffraction Imaging with Low-(30–250 eV) and High-(80–300 keV) Energy Electrons: History, Principles, and Recent Trends

Latychevskaia, Tatiana

doi:10.3390/ma13143089

Cited by 8 publications

(6 citation statements)

References 126 publications

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“…Even in the latter case, radiation damage remains a major issue and is the subject of active research. In particular, it has been suggested recently (Naydenova et al, 2019; Latychevskaia, 2020) that high-resolution imaging using lower-energy electrons may be advantageous, compared to the 200–300 keV electrons used in most modern high-resolution TEM instruments. We believe that the CHR method proposed in the present paper can be later adapted to the conditions relevant for lower-energy electron imaging, where it could provide even larger benefits due to the further reduced depth of field and improved longitudinal resolution.…”

Section: Discussion and Summarymentioning

confidence: 99%

A Method for High-Resolution Three-Dimensional Reconstruction with Ewald Sphere Curvature Correction from Transmission Electron Images

Gureyev

Paganin

Brown

et al. 2022

Microscopy and Microanalysis

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A method for three-dimensional reconstruction of objects from defocused images collected at multiple illumination directions in high-resolution transmission electron microscopy is presented. The method effectively corrects for the Ewald sphere curvature by taking into account the in-particle propagation of the electron beam. Numerical simulations demonstrate that the proposed method is capable of accurately reconstructing biological molecules or nanoparticles from high-resolution defocused images under conditions achievable in single-particle electron cryo-microscopy or electron tomography with realistic radiation doses, non-trivial aberrations, multiple scattering, and other experimentally relevant factors. The physics of the method is based on the well-known Diffraction Tomography formalism, but with the phase-retrieval step modified to include a conjugation of the phase (i.e., multiplication of the phase by a negative constant). At each illumination direction, numerically backpropagating the beam with the conjugated phase produces maximum contrast at the location of individual atoms in the molecule or nanoparticle. The resultant algorithm, Conjugated Holographic Reconstruction, can potentially be incorporated into established software tools for single-particle analysis, such as, for example, RELION or FREALIGN, in place of the conventional contrast transfer function correction procedure, in order to account for the Ewald sphere curvature and improve the spatial resolution of the three-dimensional reconstruction.

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Section: Discussion and Summarymentioning

confidence: 99%

A Method for High-Resolution Three-Dimensional Reconstruction with Ewald Sphere Curvature Correction from Transmission Electron Images

Gureyev

Paganin

Brown

et al. 2022

Microscopy and Microanalysis

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“…This recording corresponds to the first step of holography. A digital reconstruction procedure can be applied to find the shape of the object [15,16]. With more complex objects, where several fringe patterns merge, the reconstruction can also be used to determine the source size: the source is smaller than the smallest observable detail.…”

Section: Source-size Measurement In a Projection Microscopementioning

confidence: 99%

Bright sources under the projection microscope: using an insulating crystal on a conductor as electron source

Lapena

Bedrane

Degiovanni

et al. 2022

Eur. Phys. J. Appl. Phys.

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The development of bright sources is allowing technological break- throughs, especially in the field of microscopy. This requires a very ad- vanced control and understanding of the emission mechanisms. For bright electron sources, a projection microscope with a field emission tip provides an interference image that corresponds to a holographic recording. Image reconstruction can be performed digitally to form a "real" image of the object. However, interference images can only be obtained with a bright source that is small: often, an ultra-thin tip of tungsten whose radius of curvature is of the order of 10nm. The contrast and ultimate resolution of this image-projecting microscope depend only on the size of the apparent source. Thus, a projection microscope can be used to characterize source brightness: for example, analyzing the interference contrast enables the size of the source to be estimated. Ultra-thin W tips are not the only way to obtain bright sources: field emission can also be achieved by ap- plying voltages leading to a weak macroscopic electric field (< 1V/μm) to insulating micron crystals deposited on conductors with a large ra- dius of curvature (> 10μm). Moreover, analyzing the holograms reveals the source size, and the brightness of these new emitters equals that of traditional field emission sources.

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“…While there have been demonstrations of the imaging of biological samples using high-energy electron holography implementations in transmission electron microscopy (TEM) [ 47 , 48 ], the imaged structures are in general on a larger spatial scale. As the present paper focuses on holographic imaging of individual biomolecules on the nanometre and sub-nanometre level, a detailed discussion of high-energy electron holography implementations is beyond the scope of this article, for an overview of the topic we refer the reader to [ 43 , 47 , 49 , 50 ].…”

Section: Introductionmentioning

confidence: 99%

Electrospray ion beam deposition plus low-energy electron holography as a tool for imaging individual biomolecules

Ochner

Rauschenbach

Malavolti

2023

Essays in Biochemistry

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Inline low-energy electron holography (LEEH) in conjunction with sample preparation by electrospray ion beam deposition (ES-IBD) has recently emerged as a promising method for the sub-nanometre-scale single-molecule imaging of biomolecules. The single-molecule nature of the LEEH measurement allows for the mapping of the molecules’ conformational space and thus for the imaging of structurally variable biomolecules, thereby providing valuable complementary information to well-established biomolecular structure determination methods. Here, after briefly tracing the development of inline LEEH in bioimaging, we present the state-of-the-art of native ES-IBD + LEEH as a method of single-protein imaging, discuss its applications, specifically regarding the imaging of structurally flexible protein systems and the amplitude and phase information encoded in a low-energy electron hologram, and provide an outlook regarding the considerable possibilities for the future advancement of the approach.

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Holography and Coherent Diffraction Imaging with Low-(30–250 eV) and High-(80–300 keV) Energy Electrons: History, Principles, and Recent Trends

Cited by 8 publications

References 126 publications

A Method for High-Resolution Three-Dimensional Reconstruction with Ewald Sphere Curvature Correction from Transmission Electron Images

A Method for High-Resolution Three-Dimensional Reconstruction with Ewald Sphere Curvature Correction from Transmission Electron Images

Bright sources under the projection microscope: using an insulating crystal on a conductor as electron source

Electrospray ion beam deposition plus low-energy electron holography as a tool for imaging individual biomolecules

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