Holographic refractive index detector for application in microchip-based separation systems

Detection in capillary electrophoresis

“…12). Ignoring dilution from the separation process, and even with an extremely wide elution event (significant band broadening), the 3s LOD is about 875 mmole and more than an order of magnitude better than previously reported for RI detection on a chip [84].…”

“…Manz et al [84] recently reported the application of the holographic forward scatter RI detector for CE on a chip. The holographic technique is a significant advance toward developing a small-volume RI detector and eliminates the need for the capillary to serve as the optic, yet it is another arrangement of the forward-scattering refractive index technique with its limitations [77±79, 84].…”

Simultaneous LIF and retro‐reflected beam interference detection for CE

“…Unfortunately, most analytes need to be derived for their non-fluorescent property under conventional LIF detection [28,29]. For RI detection, being of universal and quantitative analysis, its comprehensive application is still far away because of its unsatisfactory sensitivity and complicated optical train [30][31][32][33][34][35].…”

Fabrication of multilayer systems combining microfluidic and microoptical elements for fluorescence detection

“…4) with the help of an AL6-2 mask aligner. 3 After development in an AZ351B solution, 4 the wafer is rinsed with DI water and dried with pressurized nitrogen. The patterned photoresist layer is then hardened (120 C, 30 min) and the unprotected poly-Si layer removed by reactive ion etching (RIE).…”

“…The detection modes in these systems can be as varied as ultraviolet (UV)-absorbance [1]- [3], refractive index measurements [4], [5], electrochemical detection [6]- [8], or laser-induced fluorescence detection (LIF) [9], [10]. Due to its selectivity and high sensitivity [11]- [13], LIF is one of the preferred detection techniques on microchemical chips.…”