Holographic Imaging and Stimulation of Neural Circuits

Yang, Wankou; Yuste, Rafael

doi:10.1007/978-981-15-8763-4_43

Cited by 5 publications

(4 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specifically, stimulation technologies have been much more limited in their degrees of freedom than modern recording technology, and thus unlikely to sufficiently control many variables of interest. For this reason, the development of multi-channel micro-LED/optrode devices [6,39,[131][132][133][134][135][136][137][138][139][140][141] and holographic optogenetic stimulation [3,4,87,89,90,142] are of particular interest. Crucially, Cleo will enable rigorous investigation of both proposed specific technologies as well as general technological capabilities to guide new interface design.…”

Section: Discussionmentioning

confidence: 99%

Bridging model and experiment in systems neuroscience with Cleo: the Closed-Loop, Electrophysiology, and Optophysiology simulation testbed

Cruzado

Willats

et al. 2023

Preprint

View full text Add to dashboard Cite

Recent advances in neurotechnology enable exciting new experiments, including novel paradigms such as closed-loop optogenetic control that achieve powerful causal interventions. At the same time, improved computational models are capable of reproducing behavior and neural activity with increasing fidelity. However, the complexities of these advances can make it difficult to reap the benefits of bridging model and experiment, such asin-silicoexperiment prototyping or direct comparison of model output to experimental data. We can bridge this gap more effectively by incorporating the simulation of the experimental interface into our models, but no existing tool integrates optogenetics, electrode recording, and flexible closed-loop processing with neural population simulations. To address this need, we have developed the Closed-Loop, Electrophysiology, and Optogenetics experiment simulation testbed (Cleo). Cleo is a Python package built on the Brian 2 simulator enabling injection of recording and stimulation devices as well as closed-loop control with realistic latency into a spiking neural network model. Here we describe the design, features, and use of Cleo, including validation of the individual system components. We further demonstrate its utility in three case studies using a variety of existing models and discuss potential applications for advancing causal neuroscience.

show abstract

Section: Discussionmentioning

confidence: 99%

Bridging model and experiment in systems neuroscience with Cleo: the Closed-Loop, Electrophysiology, and Optophysiology simulation testbed

Cruzado

Willats

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The principle of cyclicity in combination with the holographic principle means that cyclic properties (or properties of a spiral in space, and periodicity in time) should be repeated at all structural and functional levels. In the same way, like the atomic principle of the structure of matter, it should also manifest itself at all structural and functional levels, because these principles are universal or global [75][76][77][78][79][80]. Thus, the above three principles: the atomic principle of the structure of matter, the principle of cyclicity and the holographic principle, structure and combine the phenomena of animate and inanimate nature.…”

Section: Causes and Effects Of Universal Principlesmentioning

confidence: 98%

“…The cyclic organization of gas transmitters in mammals is one of the manifestations of this unity [81]. At present the ideas of cyclicity, periodicity, and holography are increasingly being used in physiology, pathophysiology, and medicine [75][76][77][78][79][80].…”

Section: Causes and Effects Of Universal Principlesmentioning

confidence: 99%

Evolutionary aspects of a typical pathological process

2023

Open J Clin Med Case Rep

View full text Add to dashboard Cite

show abstract

“…The local frequency preference in the auditory cortex is diverse, thus neighboring neurons can show widely differing tuning properties 5 . To test how activity in specific cells among an intermingled and spatially distributed co-tuned and non co-tuned cell population is balanced during auditory processing, we stimulated a small group of cells using in vivo holographic optogenetic stimulation method 20,21 while imaging AC population activity using 2-photon Ca 2+ imaging in awake mice. Stimulating small ensembles of co-tuned neurons together with the presentation of a pure tone in their preferred frequency increased their tone-evoked activity.…”

Section: Main Textmentioning

confidence: 99%

Rapid rebalancing of co-tuned ensemble activity in the auditory cortex

Kang,

Babola,

Kanold

2024

Preprint

View full text Add to dashboard Cite

Sensory information is represented by small neuronal ensembles in sensory cortices. Neuronal activity shows high trial-by-trial variability in that repeated presentation of the same stimulus, e. g., multiple presentations of the same sound activate differing ensembles in the auditory cortex (AC). How the differing ensembles interact to selectively activate to process incoming sound inputs with reduced energy is unknown. Efficient processing of complex acoustic signals requires that these sparsely distributed neuronal ensembles actively interact in order to provide a constant percept. Here, we probe interactions within and across ensembles by combiningin vivo2-photon Ca2+imaging and holographic optogenetic stimulation to study how increased activity of single cells level affects the cortical network. We stimulated a small number of neurons sharing the same frequency preference alongside the presentation of a target pure tone, further increasing their tone-evoked activity. We found that other non-stimulated co-tuned neurons decreased their tone-evoked activity while non co-tuned neurons were unaffected. This shows that co-tuned ensembles communicated and balanced their total activity across the network. The rebalanced activity due to external stimulation remained constant. These effects suggest that co-tuned ensembles in AC interact and rapidly rebalance their activity to maintain encoding homeostasis, and that the rebalanced network is persistent.

show abstract

Holographic Imaging and Stimulation of Neural Circuits

Cited by 5 publications

References 80 publications

Bridging model and experiment in systems neuroscience with Cleo: the Closed-Loop, Electrophysiology, and Optophysiology simulation testbed

Bridging model and experiment in systems neuroscience with Cleo: the Closed-Loop, Electrophysiology, and Optophysiology simulation testbed

Evolutionary aspects of a typical pathological process

Rapid rebalancing of co-tuned ensemble activity in the auditory cortex

Contact Info

Product

Resources

About