Hollow-fiber ultrafiltration for simultaneous recovery of viruses, bacteria and parasites from reclaimed water

Liu, Pengbo; Hill, Vincent R.; Hahn, Donghyun; Johnson, Trisha B.; Pan, Y.; Jothikumar, Narayanan; Moe, Christine L.

doi:10.1016/j.mimet.2011.11.007

Cited by 87 publications

(66 citation statements)

References 25 publications

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“…Ultrafiltration methods. Ultrafiltration (UF) methods rely on size exclusion and have pore sizes rated by molecular weight cutoffs which enable concentration by sieving, instead of adsorption or sedimentation (24). The ultrafilters used in this study were Rexbrane Membrane HighFlux, REXEED-25S (Asahi Kasei Kuraray Medical Co., Ltd., Japan), with a molecular weight cutoff of 29,000, surface area of 2.5 m 2 , and fiber inner diameter of 185 m. Two UF methods were included in this study:…”

Section: Methodsmentioning

confidence: 99%

Comparison of Filters for Concentrating Microbial Indicators and Pathogens in Lake Water Samples

Francy

Stelzer

Brady

et al. 2013

Appl Environ Microbiol

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Bacterial indicators are used to indicate increased health risk from pathogens and to make beach closure and advisory decisions; however, beaches are seldom monitored for the pathogens themselves. Studies of sources and types of pathogens at beaches are needed to improve estimates of swimming-associated health risks. It would be advantageous and cost-effective, especially for studies conducted on a regional scale, to use a method that can simultaneously filter and concentrate all classes of pathogens from the large volumes of water needed to detect pathogens. In seven recovery experiments, stock cultures of viruses and protozoa were seeded into 10-liter lake water samples, and concentrations of naturally occurring bacterial indicators were used to determine recoveries. For the five filtration methods tested, the highest median recoveries were as follows: glass wool for adenovirus (4.7%); NanoCeram for enterovirus (14.5%) and MS2 coliphage (84%); continuous-flow centrifugation (CFC) plus Virocap (CFC؉ViroCap) for Escherichia coli (68.3%) and Cryptosporidium (54%); automatic ultrafiltration (UF) for norovirus GII (2.4%); and dead-end UF for Enterococcus faecalis (80.5%), avian influenza virus (0.02%), and Giardia (57%). In evaluating filter performance in terms of both recovery and variability, the automatic UF resulted in the highest recovery while maintaining low variability for all nine microorganisms. The automatic UF was used to demonstrate that filtration can be scaled up to field deployment and the collection of 200-liter lake water samples.

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Section: Methodsmentioning

confidence: 99%

Comparison of Filters for Concentrating Microbial Indicators and Pathogens in Lake Water Samples

Francy

Stelzer

Brady

et al. 2013

Appl Environ Microbiol

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“…In contrast, devices in which liquid moves tangentially to the filter surface (tangential flow filtration, TFF) prevent the significant reduction of flow rates. TFF was successfully used for concentration of viruses, bacteria, and parasites from environmental samples [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Environmental surveillance of viruses by tangential flow filtration and metagenomic reconstruction

et al. 2016

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“…tangential flow filtration (TFF) and hollow-fiber ultrafiltration, based on size exclusion or entrapment of viral particles to a filter matrix, has emerged as the most promising methods for virus concentration from large volumes of water (Gibson and Schwab, 2011;Hill et al, 2005;Olszewski et al, 2005;Polaczyk et al, 2008;Rhodes et al, 2011). Furthermore, these methods have been optimized for the simultaneous concentration of multiple microorganisms (e.g., enteric bacteria, protozoa, and viruses) from diverse water matrixes, including source and finished drinking water, tap water, surface water, and reclaimed water (Gibson and Schwab, 2011;Hill et al, 2007;Hill et al, 2005;Liu et al, 2012;Morales-Morales et al, 2003;Polaczyk et al, 2008). In addition, a new type of filtration media composed of highly electropositive nanoalumina (AlOOH) fibers (~2 nm in diameter by ~250 nm in length) and microglass is currently available for concentrating EVs and other enteric viruses from large volumes of water (Gibbons et al, 2010;Ikner et al, 2011;Karim et al, 2009;Li et al, 2010).…”

Section: Detection Methodsmentioning

confidence: 99%

Polioviruses and other Enteroviruses

Betancourt¹,

Shulman²

2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

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Hollow-fiber ultrafiltration for simultaneous recovery of viruses, bacteria and parasites from reclaimed water

Cited by 87 publications

References 25 publications

Comparison of Filters for Concentrating Microbial Indicators and Pathogens in Lake Water Samples

Comparison of Filters for Concentrating Microbial Indicators and Pathogens in Lake Water Samples

Environmental surveillance of viruses by tangential flow filtration and metagenomic reconstruction

Polioviruses and other Enteroviruses

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