Hollow fiber based affinity selection combined with high performance liquid chromatography–mass spectroscopy for rapid screening lipase inhibitors from lotus leaf

Tao, Yi; Zhang, Yufeng; Wang, Yi; Cheng, Yiyu

doi:10.1016/j.aca.2013.04.058

Cited by 47 publications

(16 citation statements)

References 29 publications

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“…However, apigenin, luteolin, kaempferol and isorhamnetin showed relatively low content in lotus leaf extract (less than 1.0 mg/g). Considering the selected flavonoids were reported by different literatures, the extraction using different solvents or different varieties and parts of lotus could result in the difference of contents of these flavonoids [ 38 , 39 , 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

Molecular Structure–Affinity Relationship of Flavonoids in Lotus Leaf (Nelumbo nucifera Gaertn.) on Binding to Human Serum Albumin and Bovine Serum Albumin by Spectroscopic Method

Tang

Liu

2017

Molecules

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Lotus leaf has gained growing popularity as an ingredient in herbal formulations due to its various activities. As main functional components of lotus leaf, the difference in structure of flavonoids affected their binding properties and activities. In this paper, the existence of 11 flavonoids in lotus leaf extract was confirmed by High Performance Liquid Chromatography (HPLC) analysis and 11 flavonoids showed various contents in lotus leaf. The interactions between lotus leaf extract and two kinds of serum albumins (human serum albumin (HSA) and bovine serum albumin (BSA)) were investigated by spectroscopic methods. Based on the fluorescence quenching, the interactions between these flavonoids and serum albumins were further checked in detail. The relationship between the molecular properties of flavonoids and their affinities for serum albumins were analyzed and compared. The hydroxylation on 3 and 3’ position increased the affinities for serum albumins. Moreover, both of the methylation on 3’ position of quercetin and the C2=C3 double bond of apigenin and quercetin decreased the affinities for HSA and BSA. The glycosylation lowered the affinities for HSA and BSA depending on the type of sugar moiety. It revealed that the hydrogen bond force played an important role in binding flavonoids to HSA and BSA.

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Section: Resultsmentioning

confidence: 99%

Molecular Structure–Affinity Relationship of Flavonoids in Lotus Leaf (Nelumbo nucifera Gaertn.) on Binding to Human Serum Albumin and Bovine Serum Albumin by Spectroscopic Method

Tang

Liu

2017

Molecules

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“…Meanwhile, the different interaction modes between the inhibitors and lipase may be responsible for their varying activities. [4] …”

Section: Discussionmentioning

confidence: 97%

“…Lipase inhibitory activity was measured according to the method previously described with slight modifications [4]. A substrate solution with a concentration of 0.1 M was prepared by sonication of 4-methylumbelliferyl oleate suspended in PBS buffer (pH 6.8).…”

Section: Measurement Of Lipase Inhibitory Effectsmentioning

confidence: 99%

“…Similarly, polyphenols in oolong tea were identified to possess potential lipase inhibitory activities, including (−)-epigallocatechin, 3,5-di-O-gallate oolonghomo-bisflavan A, and theaflavin 3,3′-Ogallate [3]. In our previous work, three flavonoids from lotus leaf were identified to be lipase inhibitors and might be responsible for the weight-reducing effect of lotus leaf [4]. To the best of our knowledge, most of the natural lipase inhibitors were polyphenols, saponins, and terpenes.…”

Section: Introductionmentioning

confidence: 96%

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Ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry for screening lipase binders from different extracts of Dendrobium officinale

Tao

Cai

et al. 2015

Anal Bioanal Chem

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Pancreatic lipase plays essential roles in the digestion, transport, and processing of dietary lipids in humans. Inhibition of pancreatic lipase leading to the decrease of lipid absorption may be used for treating obesity. In the present study, a new approach of ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry was established for rapidly detecting lipase binders from different extracts of medicinal plants. Rutin, a model inhibitor of lipase, was selected to optimize the screening conditions, including ion strength, temperature, pH, and incubation time. Meanwhile, the specificity of the approach was investigated by using denatured lipase and inactive compound emodin. The optimal screening conditions were as follows: ion strength 75 mM, temperature 37 °C, pH 7.4, and incubation time 10 min. Furthermore, linearity, accuracy, precision, and matrix effect of the approach were well validated. Finally, lipase binders were screened from different extracts of Dendrobium officinale by applying the established approach and were subsequently subjected to traditional lipase inhibitory assay. Eleven lipase inhibitors were identified, eight of which, namely naringenine, vicenin II, schaftoside, isoschaftoside, isoquercetrin, kaempferol 3-O-β-D-glucopyranoside, vitexin 2″-O-glucoside, and vitexin 2″-O-rhamnoside, were reported for the first time. In addition, docking experiments were performed to determine the preferred binding sites of these new lipase inhibitors.

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“…The assay of lipase was performed according to previous method with slight modifications [ 28 ]. 25 μ L test samples and 25 μ L lipase solution were mixed together.…”

Section: Methodsmentioning

confidence: 99%

A Bioactivity‐Based Method for Screening, Identification of Lipase Inhibitors, and Clarifying the Effects of Processing Time on Lipase Inhibitory Activity of Polygonum Multiflorum

Chang

Jiang

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Traditional Chinese medicine (TCM) has been used for the treatment of many complex diseases. However, the bioactive components are always undefined. In this study, a bioactivity-based method was developed and validated to screen lipase inhibitors and evaluate the effects of processing on the lipase inhibitory activity of TCM by ultrahigh performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry and fraction collector (UHPLC/Q-TOF-MS-FC). The results showed that both Polygonum multiflorum and processed P. multiflorum extracts had inhibitory effect against lipase with IC50 values of 38.84 μg/mL and 190.6 μg/mL, respectively. Stilbenes, phenolic acid, flavonoids, and anthraquinones were considered to be the potential lipase inhibitors. Eleven potential lipase inhibitors were simultaneously determined by UHPLC. Principal component analysis (PCA) was employed in exploring the effects of processing time on lipase inhibitory activity of P. multiflorum. Compared with conventional methods, a bioactivity-based method could quantitatively analyze lipase inhibitory activity of individual constituent and provide the total lipase inhibitory activity of the samples. The results demonstrated that the activity integrated UHPLC/Q-TOF-MS-FC method was an effective and powerful tool for screening and identifying lipase inhibitors from traditional Chinese medicines.

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Hollow fiber based affinity selection combined with high performance liquid chromatography–mass spectroscopy for rapid screening lipase inhibitors from lotus leaf

Cited by 47 publications

References 29 publications

Molecular Structure–Affinity Relationship of Flavonoids in Lotus Leaf (Nelumbo nucifera Gaertn.) on Binding to Human Serum Albumin and Bovine Serum Albumin by Spectroscopic Method

Molecular Structure–Affinity Relationship of Flavonoids in Lotus Leaf (Nelumbo nucifera Gaertn.) on Binding to Human Serum Albumin and Bovine Serum Albumin by Spectroscopic Method

Ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry for screening lipase binders from different extracts of Dendrobium officinale

A Bioactivity‐Based Method for Screening, Identification of Lipase Inhibitors, and Clarifying the Effects of Processing Time on Lipase Inhibitory Activity of Polygonum Multiflorum

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