Hollow Cobalt Sulfide Nanospheres with Highly Enzyme-like Antibacterial Activities to Accelerate Infected Wound Healing

Abstract

The emergence of nanozymes presents a promising alternative to antibiotics for reactive oxygen species-mediated broad-spectrum antimicrobial purposes, but nanozymes still face challenges of low therapeutic efficiency and poor biocompatibility. Herein, we creatively prepared a novel kind of hollow cobalt sulfide (CoS) nanospheres with a unique mesoporous structure that is able to provide numerous active sites for enzyme-like reactions. The results revealed that 50 μg/mL of CoS nanospheres exhibited strong peroxidase- and oxidase-like activities under physiological conditions with the assistance of a low concentration of hydrogen peroxide (H2O2, 100 μM) while possessing highly efficient GSH-depletion ability, which endowed CoS nanospheres with triple enzyme-like properties to combat bacterial infections. The in vitro experiments demonstrated that the CoS nanozyme displayed significant antibacterial effects against both Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli). The in vivo implantation showed that the synthesized CoS effectively eliminated bacteria and promoted the recovery of infected wounds in rats while exhibiting a low cytotoxicity. This study provides a promising treatment strategy to accelerate infected wound healing.

Nanoarchitectonics of Bimetallic Cu-/Co-Doped Nitrogen–Carbon Nanozyme-Functionalized Hydrogel with NIR-Responsive Phototherapy for Synergistic Mitigation of Drug-Resistant Bacterial Infections

“…Various metallic nanomaterials including monometallic Co NPs, Cu NPs, CoS NSs, chitosan (CS)-Cu-GA NCs, MnO 2 @Ce 6 , ICG@hMnO x , bimetallic Cu 2 O-SnO 2 , MoS 2 /CoS 2 NFs, FeCo@PDA NPs, Cu 1.5 Mn 1.5 O 4 cage-like frame (CF) NSs, cobalt copper glycerate hollow nanospheres (CCG-HNSs), and trimetallic Mo/Fe@CuO 2 have been reported. In this work, bimetallic CuCo/NC exhibits POD-like, OXD-like, photothermal, and photodynamic activities, the MIC value in the CuCo/NC+H 2 O 2 system is 40 μg mL –1 , and the sterilization rate of CuCo/NC-HG+H 2 O 2 +NIR system against MRSA and Amp r E. coli is 99.79 and 99.70%, respectively.…”