1969
DOI: 10.1007/bf00268707
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Hochaufl�sende Gefrier�tzung

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1972
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Cited by 33 publications
(9 citation statements)
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“…4,6,7,8) gives a finer grain than platinumkarbon (Pt/C) replication (Figs. 3, 5, 9, lo), as reported by Bachmann et al (1969Bachmann et al ( , 1985 and Gross et al (1985). Ta/W replicas are on average 0.2 nm thick (i.e., 10-20% of the thickness of PtlC replicas as measured by a quartz crystal monitor).…”
Section: Replication Methodsmentioning
confidence: 98%
“…4,6,7,8) gives a finer grain than platinumkarbon (Pt/C) replication (Figs. 3, 5, 9, lo), as reported by Bachmann et al (1969Bachmann et al ( , 1985 and Gross et al (1985). Ta/W replicas are on average 0.2 nm thick (i.e., 10-20% of the thickness of PtlC replicas as measured by a quartz crystal monitor).…”
Section: Replication Methodsmentioning
confidence: 98%
“…Ta/W has the advantage in that it provides a finer grain, and the replicas can thus be thinner than Pt/C replicas. Hence, especially at a high magnification, Ta/W replicas describe better the outlines of the structures of interest (Bachmann et al, 1969;Gross et al, 1985;Plattner and Zingsheim, 1983;Southworth et al, 1975;Zingsheim and Plattner, 1976;Zingsheim et al, 1970). Under very high vacuum conditions (pressure < 10pgmbar) at very low temperatures (-260°C) Pt/C shadowing also gives a fine grain replica (Gross et al, 1985).…”
Section: Freeze-fracture Freeze-etch and Freeze-substitution Technomentioning
confidence: 95%
“…Walley, Cressington Scientific, Watford, U.K., personal communication). The heat load on the samples is approximately equivalent for both replication mixtures, and Ta/W is actually somewhat better in that respect (Bachmann et al, 1969). Replication can be carried out unilaterally at angles of, most commonly, around 45", or by rotary replication (Chandler, 1986), for which angles of around 20" to 25" are suggested to be optimal.…”
Section: Freeze-fracture Freeze-etch and Freeze-substitution Technomentioning
confidence: 99%
“…High-resolution shadowing with tantalum-tungsten was performed with an electron-beam evaporator designed to minimize damage to biological specimens (5,6,13). The high evaporation rates obtainable with this evaporator allowed a short evaporation time of 10 sec, and a large source-to-specimen distance of 18 cm.…”
Section: Methodsmentioning
confidence: 99%
“…Modification of an electron-bombardment gun has eliminated the obstacle to the biological application of this technique, and such high-resolution shadowing has been successfully applied in freeze-etching (5). In this study, we used an electron-bombardment evaporator to shadow monomeric and dimeric samples of tRNA with tantalumtungsten.…”
mentioning
confidence: 99%