hnRNPDL extensively regulates transcription and alternative splicing

Li, Ruth Zhen; Hou, Jing; Wei, Yaxun; Luo, Xiaotian; Ye, Yali; Zhang, Yi

doi:10.1016/j.gene.2018.11.026

Cited by 39 publications

(37 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When hnRNPDL binds the specific mRNA, it induces the decay of the molecule (Fialcowitz et al, 2005) and, for this reason, it is considered to repress the gene expression of its targets. One of its potential targets is the Cell Division Cycle and Apoptosis Regulator 1 (CCAR1) (Li et al, 2019), which acts as a key intracellular transducer for apoptosis (Rishi et al, 2003). In addition, hnRNPDL also regulates the expression of cytochrome C oxidase subunit 5B (COX5B), a mitochondrial energy-generating enzyme critical for the proper functioning of cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression

Mateo‐Otero

Sánchez

Recuero

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

A growing body of evidence suggests that paternal factors have an impact on offspring development. These studies have been mainly carried out in mice, where seminal plasma (SP) has been shown to regulate endometrial gene expression and impact embryo development and subsequent offspring health. In cattle, infusion of SP into the uterus also induces changes in endometrial gene expression, however, evidence for an effect of SP on early embryo development is lacking. In addition, during natural mating, the bull ejaculates in the vagina; hence, it is not clear whether any SP reaches the uterus in this species. Thus, the aim of the present study was to determine whether SP exposure leads to improved early embryo survival and developmental rates in cattle. To this end, Day 7 in vitro produced blastocysts were transferred to heifers (12-15 per heifer) previously mated to vasectomized bulls (n = 13 heifers) or left unmated (n = 12 heifers; control). At Day 14, heifers were slaughtered, and conceptuses were recovered to assess size, morphology and expression of candidate genes involved in different developmental pathways. Additionally, CL volume at Day 7, and weight and volume of CL at Day 14 were recorded. No effect of SP on CL volume and weight not on conceptus recovery rate was observed. However, filamentous conceptuses recovered from SP-exposed heifers were longer in comparison to the control group and differed in expression of CALM1, CITED1, DLD, HNRNPDL, PTGS2, and TGFB3. In conclusion, data indicate that female exposure to SP during natural mating can affect conceptus development in cattle. This is probably achieved through modulation of the female reproductive environment at the time of mating.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression

Mateo‐Otero

Sánchez

Recuero

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…In line with it, we performed reverse transcription (RT) with RT primer harboring 3′ adaptor sequence and randomized hexamer. Before they were used for sequencing, the purified cDNAs were amplified and stored at −80°C ( Li et al, 2019 ). As reported by the manufacturer’s instructions, the libraries were ready for high-throughput sequencing.…”

Section: Methodsmentioning

confidence: 99%

IGF2BP3 May Contributes to Lung Tumorigenesis by Regulating the Alternative Splicing of PKM

Huang

Zhang

Jiang

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

RNA binding proteins (RBPs) play a key role in genome regulation. Here we report the post-transcript regulation of IGF2BP3, which belongs to the insulin-like growth factor 2 mRNA binding protein family. We used iRIP-seq and RNA-seq to analyze the transcript regulation and alternative splicing on IGF2BP3 treated with overexpression cells and control. Overexpressed IGF2BP3 has broadly increased genes expression which involved in G-protein coupled receptor signaling pathway, positive regulation of cell proliferation, and signal transduction. IGF2BP3 regulated alternative splicing of multiple genes mainly clustered at response to hypoxia, negative regulation of transcription, and embryonic development. This study first provides alternative splicing analysis on transcription level of IGF2BP3 regulation, which laid the foundation for later research on IGF2BP3 critical functions.

show abstract

“…hnRNPDL acts as a regulator of pre-mRNA splicing and nuclear export. HNRNPDL mutations are thought to cause defects in mRNA biogenesis and metabolism [5].This DNA/RNA-binding protein has been shown to localize in the cytoplasm and in the nucleus [6,7]. Data from Vieira et al 2014, suggested that the translocation of the protein from the cytoplasm to the nucleus might be impaired by the p.(Asp378Asn)mutation.…”

Section: Introductionmentioning

confidence: 99%

HNRNPDL-related muscular dystrophy: expanding the clinical, morphological and MRI phenotypes

Berardo¹,

Lornage

Johari

et al. 2019

J Neurol

View full text Add to dashboard Cite

Autosomal dominant limb girdle muscular dystrophy D3 HNRNPDL-related is a rare dominant myopathy caused by mutations in HNRNPDL. Only three unrelated families have been described worldwide, a Brazilian and a Chinese carrying the mutation c.1132G>A p.(Asp378Asn), and one Uruguayan with the mutation c.1132G>C p. (Asp378His), both mutations occurring in the same codon. The present study enlarges the clinical, morphological and muscle MRI spectrum of AD-HNRNPDL-related myopathies demonstrating the significant particularities of the disease. We describe two new unrelated Argentinean families, carrying the previously reported c.1132G>C p.(Asp378His) HNRNPDL mutation. There was a wide phenotypic spectrum including oligo-symptomatic cases, pure limb girdle muscle involvement or distal lower limb muscle weakness. Scapular winging was the most common finding, observed in all patients. Muscle MRIs of the thigh, at different stages of the disease, showed particular involvement of adductor magnus and vastus besides a constant preservation of the rectus femoris and the adductor longus muscles, defining a novel MRI pattern. Muscle biopsy findings were characterized by the presence of numerous rimmed vacuoles, cytoplasmic bodies, and abundant autophagic material at the histochemistry and ultrastructural levels. HNRNPDL-related LGMD D3 results in a wide range of clinical phenotypes from the classic proximal form of LGMD to a more distal phenotype. Thigh MRI suggests a specific pattern. Codon 378 of HNRNPDL gene can be considered a mutation hotspot for HNRNPDL-related myopathy. Pathologically, the disease can be classified among the autophagic rimmed vacuolar myopathies as with the other multisystem proteinopathies.

show abstract

hnRNPDL extensively regulates transcription and alternative splicing

Cited by 39 publications

References 62 publications

Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression

Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression

IGF2BP3 May Contributes to Lung Tumorigenesis by Regulating the Alternative Splicing of PKM

HNRNPDL-related muscular dystrophy: expanding the clinical, morphological and MRI phenotypes

Contact Info

Product

Resources

About