HLTF resolves G4s and promotes G4-induced replication fork slowing to maintain genome stability

Bai, Gongshi; Endres, Theresa; Kühbacher, Ulrike; Mengoli, Valentina; Greer, Briana H.; Peacock, Emma M.; Newton, Matthew D.; Stanage, Tyler; Dello Stritto, Maria Rosaria; Lungu, Roxana; Crossley, Magdalena P.; Sathirachinda, Ataya; Cortez, David; Boulton, Simon J.; Cejka, Petr; Eichman, Brandt F.; Cimprich, Karlene A.

doi:10.1016/j.molcel.2024.07.018

Molecular Cell

2024

DOI: 10.1016/j.molcel.2024.07.018

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HLTF resolves G4s and promotes G4-induced replication fork slowing to maintain genome stability

Gongshi Bai,

Theresa Endres,

Ulrike Kühbacher

et al.

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2024

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Strand dependent bypass of DNA lesions during fork reversal by ATP-dependent translocases SMARCAL1, ZRANB3, and HLTF

Adolph,

Warren,

Couch

et al. 2024

Preprint

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During DNA replication, the replisome encounters obstacles including DNA lesions, transcription-replication conflicts, and other sources of replication stress. These obstacles must be efficiently overcome to complete DNA synthesis and minimize genome instability. One pathway to tolerate replication stress is replication fork reversal, in which parental template DNA strands are reannealed and a nascent-nascent DNA duplex is formed. Several enzymes promote replication fork reversal, including the ATP-dependent translocases SMARCAL1, ZRANB3, and HLTF. How these enzymes translocate on DNA that contains fork-stalling lesions is unknown. Here, we examined the abilities of SMARCAL1, ZRANB3, and HLTF to tolerate various lesions on leading or lagging template strands. We demonstrate that SMARCAL1 and ZRANB3 are selectively inhibited by lesions on the leading template strand, whereas HLTF is insensitive to bulky lesions on either strand. These results suggest that SMARCAL1 and ZRANB3 contact the leading strand during fork reversal and therefore are more sensitive to inhibition by bulky lesions on this strand. In contrast, HLTF DNA translocation is inherently insensitive to DNA lesions. These biochemical differences between the fork reversal enzymes provide insights into their mechanism of DNA remodeling and suggest they may act in lesion-specific contexts.

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Strand dependent bypass of DNA lesions during fork reversal by ATP-dependent translocases SMARCAL1, ZRANB3, and HLTF

Adolph,

Warren,

Couch

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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HLTF resolves G4s and promotes G4-induced replication fork slowing to maintain genome stability

Cited by 1 publication

References 125 publications

Strand dependent bypass of DNA lesions during fork reversal by ATP-dependent translocases SMARCAL1, ZRANB3, and HLTF

Strand dependent bypass of DNA lesions during fork reversal by ATP-dependent translocases SMARCAL1, ZRANB3, and HLTF

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