HLA typing by NGS in the clinical lab: our one and a half years experience

Targeted Next-Generation Sequencing for Human Leukocyte Antigen Typing in a Clinical Laboratory: Metrics of Relevance and Considerations for Its Successful Implementation

“…Two analysis programs were used to genotype the NGS data, Target (version 1.7/1.8, Omixon) and NGSengine (version 1.3/1.6/ 1.9/2.1, GenDX, Utrecht, the Netherlands). 16 The 2 genotyping programs used for analysis to safeguard against systematic analysis errors agreed in 94.8% (26 508 of 27 488) of the best-match allele calls up to the third field.…”

“…[13][14][15] HLA typing by NGS is also being used by laboratories serving solid organ transplant programs because high-resolution typing of the donor and recipient helps in the interpretation of anti-HLA antibody specificities. 16 We describe a systematic presentation of the different elements that constitute the preanalytic, analytic, and postanalytic systems necessary for the practice of HLA typing by NGS in clinical diagnostic laboratories. This will be illustrated with a validated, robust, reproducible method for HLA typing by NGS developed at the Children's Hospital of Philadelphia (CHOP; Philadelphia, Pennsylvania) 9,17 and used clinically since December 2013.…”