HLA-G Gene Polymorphism in Human Placentas: Possible Association of G*0106 Allele with Preeclampsia and Miscarriage

Abstract

Definite causes for several pathologies of pregnancy remain unknown. In light of several recent studies, however, diminished or aberrant HLA-G expression may be associated with certain complication of pregnancy and be linked to HLA-G polymorphism. We analyzed DNA from 60 normal placentas (controls), 140 placentas from miscarriage, 36 placentas from preeclampsia, 76 placentas from fetal hypotrophy, and 34 placentas with hypoxia for variations in coding regions (allelic groups G*0101 to G*0107) and the 14-bp deletion/insertion into the 3'-untranslated region. No statistically significant differences were observed in the distribution of allelic group between pathological placentas and controls with the exception of G*0106 allele frequency in preeclamptic compared with control placentas (21.2% and 6.6%, respectively). A greater frequency of this allele also was observed in the two subgroups of miscarriage and hypoxia compared with that in controls. In addition, presence of the 14-bp sequence was prominent in preeclampsia compared with controls (60.8% vs. 35%, respectively), and homozygotes with deletion were not detected in the pathology. The results suggest that the G*0106 allele, which is coupled with the presence of the 14-bp sequence, contributes and/or is a relevant marker in some specific complications of pregnancy, especially preeclampsia.

Leukocyte driven-decidual angiogenesis in early pregnancy

“…The genetic haplotypes associated with these clinical problems are those in which KIR fail to activate uNK cells. [68][69][70] Using explant studies, we recently observed that interactions with trophoblasts stimulate uNK survival and proliferation and decrease uNK cell expression of the activating receptor CD314/NKG2D (Zhang J, Dunk C, Lye JS et al, submitted manuscript). The study of human endometrial and decidual leukocytes is challenging with the global positioning of maternal leukocytes within implantation sites undefined.…”

Association of 14-bp insertion/deletion polymorphism of HLA-G gene with idiopathic recurrent miscarriages in infertility center patients in Yazd, Iran

“…Perhaps HLA-G as an exclusive factor has a very modest effect in relation to risk for RM (Moreau et al, 2008). According to Yan et al (2006), a significantly different distribution of the 14-bp genotype exists among Chinese, Danish and Indian RM populations and the discrepancy may be a result of ethnic variation (see Table 3).…”

Population genetic structure of HLA-G gene in North-west Region of Russian Federation

“…Для идентификации основных классов алле-лей (G*0101, G*0102, G*0103, G*0104, G*0105N, G*0106 и G*0107) была разработана собственная сис-тема праймеров (за основу брали последовательности олигонуклеотидов французских исследователей (Moreau et al, 2008). Эти праймеры позволяли амплифицировать фрагменты анализируемых аллелей в моноплексной ре-акции и практически не отличались друг от друга по раз-мерам, что не позволяло в дальнейшем использовать их для проведения мультиплексной ПЦР.…”