HLA-G expression in trophoblast cells circulating in maternal peripheral blood during early pregnancy

Wijk, Inge J. van; Griffioen, S.; Tjoa, May Lee; Mulders, Monique A.M.; Vugt, J.M.G. van; Loke, Y.W.; Oudejans, Cees B.M.

doi:10.1067/mob.2001.112973

Cited by 49 publications

(32 citation statements)

References 22 publications

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“…Most research has focused on the isolation and identification of nucleated red blood cells, culminating in the National Institutes of Health Fetal Cell Study (NIFTY) trial, in which the efficacy of fetal cells for the determination of fetal gender and diagnosis of trisomy 21 in the fetus was investigated [2] . Although several studies have established the presence of trophoblast cells in the blood of pregnant women [3,4] , less research has been performed on this particular cell type, in part due to the lack of trophoblast-specific antibodies.…”

Section: Introductionmentioning

confidence: 99%

Antibodies to Trophoblast Antigens HLA-G, Placenta Growth Factor, and NeuroD2 Do Not Improve Detection of Circulating Trophoblast Cells in Maternal Blood

et al. 2006

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Objectives: Non-invasive prenatal diagnosis using circulating fetal trophoblast cells has been challenging due to lack of a reproducible trophoblast-specific antibody. We investigated the use of three trophoblast cell-specific antibodies, HLA-G, placenta growth factor, and neuroD2, for the isolation of trophoblast cells from the maternal circulation. Methods: Trophoblast cells were isolated by density centrifugation from maternal blood samples (gestational age 10–20 weeks, n = 9). All women were carrying a male fetus. Following immunocytochemical staining with the trophoblast-specific antibodies, fluorescent in situ hybridization was performed, to verify whether any stained cells were indeed fetal. Results: The HLA-G antibody had a ubiquitous staining pattern, which was not specific for trophoblast cells. Neither the placenta growth factor nor the neuroD2 antibodies were able to identify any trophoblast cells. Following fluorescent in situ hybridization, no male cells were detected on any of the slides. Conclusion: The antibodies used in this study were unable to improve detection of trophoblast cells in the maternal circulation.

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Section: Introductionmentioning

confidence: 99%

Antibodies to Trophoblast Antigens HLA-G, Placenta Growth Factor, and NeuroD2 Do Not Improve Detection of Circulating Trophoblast Cells in Maternal Blood

et al. 2006

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“…Trophoblast cells have been isolated from maternal blood by several different methods related to surface antigen expression, e.g., HLA-G, and cell size (van Wijk et al 2001a;Vona et al 2002). Proof of fetal origin as well as detection of aneuploidy and polyploidy were accomplished with fluorescence in situ hybridization (FISH) and PCR-based methods (van Wijk et al 2001a,b;Vona et al 2002).…”

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confidence: 99%

Trophoblasts Isolated from the Maternal Circulation: In Vitro Expansion and Potential Application in Non-invasive Prenatal Diagnosis

Guetta

Gutstein-Abo

Barkai

2005

J Histochem Cytochem.

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S U M M A R YPrenatal diagnosis based on rare fetal cells in maternal blood is currently not a feasible option. An effort was made to improve cell yields by targeting trophoblast cells. After sorting, the HLA-G-positive cell fraction was analyzed directly or after culture. In situ hybridization technology was applied to prove fetal cell source in samples from women carrying a male fetus and to predict gender in samples without previous knowledge of fetal sex. In vitro culture led to a significant increase in fetal cells and accurate gender prediction in 93% of these samples. This approach might be useful for non-invasive prenatal diagnosis.

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“…However, only 4.25% of the nuclei analyzed by FISH showed a Y-signal [15]. Human leukocyte antigen G has also been successfully used to enrich circulating trophoblasts from maternal circulation and use those cells for aneuploidy detection [16][17][18]. Other studies have relied on size-based isolation of fetal trophoblastic cells and used those cells for prenatal diagnosis [19].…”

Section: Trophoblastsmentioning

confidence: 99%

Fetal Cells in Maternal Blood For Prenatal Diagnosis: A Love Story Rekindled

Singh¹,

Hatt²,

Ravn³

et al. 2017

Biomark. Med.

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HLA-G expression in trophoblast cells circulating in maternal peripheral blood during early pregnancy

Cited by 49 publications

References 22 publications

Antibodies to Trophoblast Antigens HLA-G, Placenta Growth Factor, and NeuroD2 Do Not Improve Detection of Circulating Trophoblast Cells in Maternal Blood

Antibodies to Trophoblast Antigens HLA-G, Placenta Growth Factor, and NeuroD2 Do Not Improve Detection of Circulating Trophoblast Cells in Maternal Blood

Trophoblasts Isolated from the Maternal Circulation: In Vitro Expansion and Potential Application in Non-invasive Prenatal Diagnosis

Fetal Cells in Maternal Blood For Prenatal Diagnosis: A Love Story Rekindled

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