HLA‐DR gene frequencies in the Japanese population obtained by oligonucleotide genotyping

Obata, Fumiya; Ito, Koichi; Kaneko, Toshihiko; Yang, Yong‐Guang; Onda, Kelji; Itô, Ichiro; Yabe, Noriko; Watanabo, Koji; Kashiwagi, Noboru

doi:10.1111/j.1399-0039.1991.tb02025.x

Cited by 21 publications

(9 citation statements)

References 27 publications

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“…tion with sequence-specific oligonucleotides (SSOs) have greatly facilitated the DNA typing of HLA (1 6). In previous studies, we identified substantially all DR types in the Japanese population without any 'blank' types by SSO-DNA typing (17). This is in contrast to the finding that about 10% of the Japanese population have been types as 'DR blank' at serological workshops (18,19).…”

Section: Jntroductioncontrasting

confidence: 68%

“…Genomic DNA was isolated from nucleated peripheral blood cells and subjected to 30 cycles of the polymerase chain reaction (PCR) with FPRl and DRBAMPl primers (20). The amplified DNA was dot-blotted onto nylon membranes and hybridized with 29 different sequence-specific oligonucleotides (SSOs) directed against various DR types as reported by Obata et al (17). DR types identified by the DNA typing were classified into three different levels of DR-definition, i.e., 27, 13 or 11 DR types (see Table 1).…”

Section: Dna Typingmentioning

confidence: 99%

“…Differences between the DR gene frequencies of URSA patients and those of the normal Japanese population (17) were examined by chi-squared test on the basis of the observed number of DR types. Fisher's exact probability test was useld when the observed number of DR types was 5 or less.…”

Section: Statisticsmentioning

confidence: 99%

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Analysis of HLA‐DR types of unexplained recurrent spontaneous aborters in the Japanese population by oligonucleotide‐DNA typing

et al. 1992

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To examine whether unexplained recurrent spontaneous abortion (URSA), defined as 2 or more consecutive spontaneous abortions, is correlated with a particular DR type in the Japanese population, we determined the HLA-DR types of 82 primary aborters and 21 secondary aborters by DNA typing utilizing the polymerase chain reaction (PCR) and hybridization with sequence-specific oligonucleotides (SSOs). The DR gene frequencies of the patient group were compared with those of a normal group at three different levels of DR-definition (27, 13 and 11 DR types). At none of the three levels of comparison was any particular DR type with a frequency differing significantly between the patient and normal groups detected in Japanese URSA patients. Furthermore, we examined whether URSA was correlated with the degree of compatibility of HLA-DR antigen within patients and their husbands. Comparison of the DR compatibility between patients and normal couples was made in two different ways, i.e., comparison of the numbers of couples with mismatches and comparison of the average number of mismatches. For either of these two comparisons, we observed no difference in DR compatibility between patients and normal couples. Our results suggest that URSA is not correlated with any particular DR type and that the condition cannot be explained simply by DR compatibility between husband and wife.

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Section: Jntroductioncontrasting

confidence: 68%

Section: Dna Typingmentioning

confidence: 99%

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Analysis of HLA‐DR types of unexplained recurrent spontaneous aborters in the Japanese population by oligonucleotide‐DNA typing

et al. 1992

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“…A 270-bp amplified DNA product was verified by electrophoresis in a 2% agarose gel containing ethidium bromide and was visualized under UV light. Hybridization was performed as described previously using a panel of sequence-specific oligonucleotide probes (SSOP) (33)(34)(35). The sequence-specific primer (SSP)-PCR using ARMS (European patent 0332435, U.S. patent 5595890, under license from AstraZeneca, Wilmington, DE) technology was used for high resolution of HLA-DRB1, HLA-DQB1, and HLA-DPB1 typing.…”

Section: Dna-based Typingmentioning

confidence: 99%

Differential Distribution of HLA-DQβ/DRβ Epitopes in the Two Forms of Guillain-Barré Syndrome, Acute Motor Axonal Neuropathy and Acute Inflammatory Demyelinating Polyneuropathy (AIDP): Identification of DQβ Epitopes Associated with Susceptibility to and Protection from AIDP

Magira

Papaioakim²,

Nachamkin³

et al. 2003

The Journal of Immunology

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Guillain-Barré syndrome (GBS), an acute, immune-mediated paralytic disorder affecting the peripheral nervous system, is the most common cause of acute flaccid paralysis in the postpolio era. GBS is classified into several subtypes based on clinical and pathologic criteria, with acute inflammatory demyelinating polyneuropathy (AIDP) and acute motor axonal neuropathy (AMAN) being the most common forms observed. To better understand the pathogenesis of GBS and host susceptibility to developing the disease, the distribution of HLA class II Ags along with the seroreactivity to Campylobacter jejuni were investigated in a population of GBS patients from northern China. Using DNA-based typing methods, 47 patients with AMAN, 25 patients with AIDP, and 97 healthy controls were studied for the distribution of class II alleles. We found that the DQβ RLD55–57/ED70–71 and DRβ E9V11H13 epitopes were associated with susceptibility to AIDP (p = 0.009 and p = 0.004, respectively), and the DQβ RPD55–57 epitope was associated with protection (p = 0.05) from AIDP. These DQβ/DRβ positional residues are a part of pockets 4 (DQβ 70, 71, DRβ13), 6 (DRβ11), and 9 (DQβ 56, 57, DRβ9); have been demonstrated to be important in peptide binding and T cell recognition; and are associated with other diseases that have a pathoimmunological basis. Class II HLA associations were not identified with AMAN, suggesting a different immunological mechanism of disease induction in the two forms of GBS. These findings provide immunogenetic evidence for differentiating the two disease entities (AMAN and AIDP) and focuses our attention on particular DRβ/DQβ residues that may be instrumental in understanding the pathophysiology of AIDP.

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“…A 270-bp amplified DNA product was verified by electrophoresis in a 2% agarose gel containing ethidium bromide, and was visualized under UV light. Hybridization was performed as described elsewhere (18,19), using a panel of SSOPs (20).…”

Section: Dna Extraction and Ssop Typingmentioning

confidence: 99%

Human Leukocyte Antigen Class II Amino Acid Epitopes

Rossman

Stubbs

Lee

et al. 2002

Am J Respir Crit Care Med

116

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Chronic beryllium disease (CBD) is a hypersensitivity granulomatosis characterized by beryllium hypersensitivity (BH) and mediated by CD4+ T cells. However, all individuals with BH may not develop CBD. To examine the role of the three different human leukocyte antigen (HLA) Class II isotypes in BH with (CBD) and without clinical disease (BHWCD), we performed DNA-based typing of HLA-DPB1, HLA-DQB1, and HLA-DRB1 loci on 55 subjects with BH (25 with established CBD and 30 with BHWCD), and compared this with the results for 82 beryllium-exposed workers with no evidence of BH. The allele distribution was utilized to identify candidate amino acid epitopes that differed between the study groups. HLA-DPB1-E69 was the most important marker for BH, and did not differentiate BHWCD from CBD. A significant association with CBD was observed with HLA-DQB1-G86 (p(corr) < 0.04), and HLA-DRB1-S11 was significantly increased in CBD as compared with BHWCD (p < 0.03). These observations suggest that HLA-DPB1-E69 is a marker for susceptibility to BH and not just a progression marker for CBD. In addition, HLA amino acid epitopes on HLA-DRB1 and -DQB1, in concert with or independently of HLA-DPB1-E69, may be associated with progression to CBD.

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HLA‐DR gene frequencies in the Japanese population obtained by oligonucleotide genotyping

Cited by 21 publications

References 27 publications

Analysis of HLA‐DR types of unexplained recurrent spontaneous aborters in the Japanese population by oligonucleotide‐DNA typing

Analysis of HLA‐DR types of unexplained recurrent spontaneous aborters in the Japanese population by oligonucleotide‐DNA typing

Differential Distribution of HLA-DQβ/DRβ Epitopes in the Two Forms of Guillain-Barré Syndrome, Acute Motor Axonal Neuropathy and Acute Inflammatory Demyelinating Polyneuropathy (AIDP): Identification of DQβ Epitopes Associated with Susceptibility to and Protection from AIDP

Human Leukocyte Antigen Class II Amino Acid Epitopes

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