HLA‐DR Antigens: Structure, Separation of Subpopulations, Gene Cloning and Function

The MHC is central to the adaptive immune response. The human MHC class II is encoded by three different isotypes, HLA-DR, -DQ, and -DP, each being highly polymorphic. In contrast to HLA-DR, the intracellular assembly and trafficking of HLA-DP molecules have not been studied extensively. However, different HLA-DP variants can be either protective or risk factors for infectious diseases (e.g. hepatitis B), immune dysfunction (e.g. berylliosis), and autoimmunity (e.g. myasthenia gravis). Here, we establish a system to analyze the chaperone requirements for HLA-DP and to compare the assembly and trafficking of HLA-DP, -DQ, and -DR directly. Unlike HLA-DR1, HLA-DQ5 and HLA-DP4 can form SDS-stable dimers supported by invariant chain (Ii) in the absence of HLA-DM. Uniquely, HLA-DP also forms dimers in the presence of HLA-DM alone. In model antigen-presenting cells, SDS-stable HLA-DP complexes are resistant to treatments that prevent formation of SDS-stable HLA-DR complexes. The unexpected properties of HLA-DP molecules may help explain why they bind to a more restricted range of peptides than other human MHC class II proteins and frequently present viral peptides.

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“…5A, lanes 3-5). Rather, the DR␣ doublet is most likely due to two differently glycosylated pools (30), similar to that seen for DM␣ (20).…”

Section: Differences In the Formation Of Sds-stable Hla -Dp -Dq Andmentioning

confidence: 94%

HLA-DP, HLA-DQ, and HLA-DR Have Different Requirements for Invariant Chain and HLA-DM

Lith

McEwen-Smith

Benham

2010

Journal of Biological Chemistry

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“…The induction of IgE synthesis depends on HLA class II antigens (Vercelli et al, 1989), and their expression and ICAM-1 expression on monocytes are critical in T cellmonocyte interactions (Shackelfold et al, 1982;Gonwa et al, 1983;Van Seventer et al, 1990;Damle et al, 1992). The effect of LBI on the expression of HLA-class II antigens and ICAM-1 on monocytes therefore was investigated.…”

Section: Effect Of Lbi On Df Antigen-induced Il-4 Secretionmentioning

confidence: 99%

“…The expression of HLA-class II antigens and ICAM-1 on monocytes therefore was examined because it has been suggested that these molecules on antigen-presenting cells are crucial in cell-mediated immune responses, including cytokine production (Shackelfold et al, 1982;Gonwa et al, 1983;Van Seventer et al, 1990;Damle et al, 1992). As the results presented in Table 1 show, LBI does not markedly affect the expression of HLA-DR, -DQ, -DP, or ICAM-l on monocytes.…”

Section: Effect Of Lbi On Df Antigen-induced Il-4 Secretionmentioning

confidence: 99%

Modulation of mite antigen‐induced immune responses by lecithin‐bound iodine in peripheral blood lymphocytes from patients with bronchial asthma

Kawano

Noma

1995

British J Pharmacology

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“…The human equivalent of rodent Ir genes occupies 3 distinct regions on the short arm of chromosome 6, which code for 3 distinct families of immune response-associated, Ia molecules: HLA-SB, HLA-DC, and HLA-DR (42)(43)(44)(45). Only reagents capable of depicting polymorphic determinants in HLA-DR molecules are routinely accessible.…”

Section: Discussionmentioning

confidence: 99%

Anticollagen antibodies and immune response gene products in rheumatoid arthritis

Kerwar

Garovoy

Fye

et al. 1984

Arthritis & Rheumatism

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Circulating antibodies to native and denatured types I and I1 human and bovine collagens were assayed in patients with rheumatoid arthritis (RA), patients with other rheumatic diseases, and normal individuals. A subgroup of this population was also assayed for reactivity with typing reagents which detect determinants (MT and HLA-DR) present in human immune response gene products. The mean titers of antibodies to each collagen tested were not significantly higher in RA patients when compared with patients who had other rheumatic diseases. Although both MT3 and MT4 were significantly associated with RA, there was no significant association between the anticollagen antibodies and any MT type or HLA-DR4. These studies raise a question concerning

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HLA‐DR Antigens: Structure, Separation of Subpopulations, Gene Cloning and Function

Cited by 251 publications

References 132 publications

HLA-DP, HLA-DQ, and HLA-DR Have Different Requirements for Invariant Chain and HLA-DM

HLA-DP, HLA-DQ, and HLA-DR Have Different Requirements for Invariant Chain and HLA-DM

Modulation of mite antigen‐induced immune responses by lecithin‐bound iodine in peripheral blood lymphocytes from patients with bronchial asthma

Anticollagen antibodies and immune response gene products in rheumatoid arthritis

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