HLA-DQA1 Introns 2 and 3 Sequencing: DQA1 Sequencing-Based Typing and Characterization of a Highly Polymorphic Microsatellite at Intron 3 of DQA1*0505

A new DQA1 allele (DQA1*0510) in a Spanish celiac disease patient

“…Intron 3 analysis showed a previously described DQA1* 0505‐specific microsatellite (3). This fact, together with sequence alignment, strongly suggests DQA1*0510 has been generated from DQA1*0505/09 by a nucleotide point mutation.…”

“…HLA‐DQA1 and ‐DQB1 typing of a celiac disease patient (CTM‐8005076) by PCR‐SSO (Tepnel Lifecodes, Stamford, CT) using Luminex technology (Luminex Corporation, Austin, TX), rendered an inconclusive typing for DQA1 locus. Exons 2 and 3 sequencing was performed by using procedures already described (3). Sequence for DQA1*05‐specific amplification product was identical to DQA1*0505/09 with a point mutation at position 298 in exon 2.…”

Group-Specific Amplification of cDNA From DRB1 Genes. Complete Coding Sequences of Partially Defined Alleles and Identification of the New Alleles DRB1*040602, DRB1*111102, DRB1*080103, and DRB1*0113

“…The PCR profile was initial TaqGold enzyme (Applied Biosystems, Foster City, CA) activation for 10 minutes at 96°C, and then 15 cycles of 20 seconds at 96°C, 30 seconds at 62°C, and 30 seconds at 72°C, and 20 cycles of 20 seconds at 96°C, 30 seconds at 60°C, and 30 seconds at 72°C. For DQA1 and DQB1, SBT procedures have been already reported [8,9], and they were used with minor modifications.…”