HLA‐DPB1 oligonucleotide typing of a Southwest German Caucasian population

Abstract

HLA-DP genotyping with sequence-specific oligonucleotides can detected known sequence variations in the polymorphic segments of the DPB1 second exon. Since the allelic polymorphism of the 22 published alleles is based on recombination of sequence motifs from six variable regions, DPB1 typing depends on the reactivity pattern of many different probes rather than from typing with single allele-specific probes. By computer simulation, we have previously shown that the minimal set of probes to define the 22 different alleles and most of the heterozygous combinations is 18. Here we describe HLA-DPB1 typing results and allele frequencies in a panel of 200 unrelated Caucasians from Southwest Germany. The result confirmed the power of the new HLA-DPB1 typing method, but we failed to detect three of the previously described alleles in our panel. To accommodate with the observed 19 different alleles, the sequence and hybridization conditions of 17 oligonucleotide probes are given, which are able to differentiate all except two, resolved by group-specific amplification, of the 190 possible heterozygous phenotypes.

HLA—DPB1 alleles in a population from North India and description of a new variant (DPBl*5601)

“…To investigate the extent of polymorphism at the DPBl locus in the North Indian population we have studied some leprosy patients, even though they were not included in the calculation of frequencies for the normal population. DPB1*0401 was found to be the most frequent allele in North Indians as has been found in Caucasoid populations (4,8,9). Interestingly DPB l "0501, the most frequent allele in North Chinese (10) and South Chinese ( 5 ) was found with a very low frequency (2%) in the present study, probably suggesting that there is only a small extent of mongoloid admixture in North India.…”

HLA-DP genetic variation, proxies for early life immune modulation and childhood acute lymphoblastic leukemia risk

“…[32][33][34] Higher frequencies for DPB1*01:01 (more consistent with the UKCCS observations) have been reported in studies of whites residing in Europe, including populations of Germany (8.0%), France (6.6%), and Great Britain (7.5%). [35][36][37] Similarly, the allele frequency of DPB1*02:01, one of the alleles driving the DP2 [32][33][34][35][36][37] Variation in DPB1 allele frequencies between populations is an indication of the potential diversity in underlying genetic structure and that the populations may have evolved under different selective pressures. In adaptive immunity, the HLA class II molecule selectively binds to the antigen and together with the T-cell receptor, forms a specialized complex that activates a cascade of intercellular signals that are specifically designed to elicit a productive immune response.…”

New Diagnostic Methods in Oncology and Hematology

“…The PCR-SSOP can be set up in two different formats, the product dot blot [98][99][100][102][103][104][105][107][108][109][110][111][112]117,119,[121][122][123][124][125][126][127] and the reverse dot blot [101,106,[113][114][115][116]118]. In the conventional product dot blot approach, the PCR products of different samples are immobilized on a carrier matrix (e.g.…”

“…Many alternative methods for conventional product dot blot PCR-SSOP have been described in the literature which differ mainly in the length and sequence of probes, the solvent used for washing steps, the reporter molecule and its detection [98][99][100][102][103][104][105][107][108][109][110][111][112]117,119,[121][122][123][124][125][126][127]. The most relevant product dot blot protocol for serology-equivalent HLA class I and highresolution HLA class II PCR-SSOP typing has been established and extensively evaluated during the 12th IHWC.…”