HLA Class-II Immune Response Genes and Products in Leprosy

Vries, R. R. P. De; Eden, W. (Willem) van; Ottenhoff, T.H.M.

doi:10.1159/000318418

Cited by 15 publications

(17 citation statements)

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“…The presentation of M. leprae by EBV-BLCL is restricted by HLA-DR (associated epitopes) as was already known for PBMNC as APC [16]. This is illustrated in Fig.…”

Section: Methodsmentioning

confidence: 69%

“…Moreover, these EBV-BLCL enable us to study in detail the HLA molecules and epitopes that restrict the presentation of antigens [10,11], using biochemical (DNA and protein analyses) and immunological (serology and cellular) typing techniques. Our interest in studying the role of HLA class II molecules in T-cell reactivity against mycobacteria [10], in particular M. leprae [16], led us to study whether EBV-BLCL can present M. leprae antigens. In this report we show that this is indeed so for both autologous and HLA-D/DR matched T-cell lines and clones.…”

mentioning

confidence: 99%

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Epstein‐Barr Virus‐Transformed B Cell Lines Present M. Leprae Antigens to T Cells

1985

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We have investigated whether or not Epstein-Barr virus-transformed lymphoblastoid B cell lines (EBV-BLCL) are able to present Mycobacterium leprae (M. leprae) to antigen-reactive T cell lines and clones. Such EBV-BLCL would provide us with a homogeneous and unlimited source of antigen-presenting cells. Antigen-triggered proliferation of T cells has been studied with co-cultures either with autologous or allogeneic irradiated EBV-BLCL. Our results show that EBV-BLCL are able to present M. leprae as efficiently as peripheral blood mononuclear cells, and that they also function in an HLA-DR-restricted fashion. Apart from their possible in vivo relevance, these results have important practical implications, in particular for the generation and study of M. leprae-reactive T-cell clones.

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“…The presentation of M. leprae by EBV-BLCL is restricted by HLA-DR (associated epitopes) as was already known for PBMNC as APC [16]. This is illustrated in Fig.…”

Section: Methodsmentioning

confidence: 69%

mentioning

confidence: 99%

Epstein‐Barr Virus‐Transformed B Cell Lines Present M. Leprae Antigens to T Cells

1985

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“…Studies with mice have indicated the existence of low and high responders both in cellular and humoral responses to mycobacterial antigens [16,[30][31][32]. It has been shown also that resistance or susceptibility to mycobacterial infections in humans and in mice is determined genetically [33][34][35][36]. Other studies using several fractionated mycobacterial antigens showed that T-cell stimulation profiles vary among individuals with no clear segregating pattern between infected subjects, immune contacts or control vaccinates [24.…”

Section: Discussionmentioning

confidence: 99%

Cellular and Humoral Immune Responses of Cattle to Purified Mycobacterium bovis Antigens

et al. 1994

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Cellular responses to several purified antigens of Mycobacterium bovis were examined in experimentally infected cattle over a period of 36 months, using in vitro cellular proliferation and interferon-gamma assays. These antigens (12, 19, 22a, b, 24, 25, 30, 32, 39, 65 and 70 kDa) included the majority of M. bovis protein antigens described to date and are highly homologous to those purified from M. tuberculosis. Cellular responses were examined at 3-month time intervals during the 36-month course of infection. All purified antigens induced cellular immune responses in the infected animals. The onset and magnitude of response to individual antigens varied among the animals. At any specific time during the period of infection one or more antigens appeared to be immunodominant but the immunodominance profile changed as the infection progressed. Humoral immune responses were low or absent in the first half of the infection period, but increased substantially for some of the antigens during the second half. Variation was observed among the different animals as to which antigens they recognized.

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“…7,8 This region harbors the HLA gene cluster, which has been extensively studied for its role in leprosy pathogenesis. [9][10][11][12] Candidate gene studies have detected association of leprosy with both class I and II genes. However, class II genes, especially the DRB1 locus, have been found more consistently associated with leprosy.…”

Section: Introductionmentioning

confidence: 99%

HLA-DRB104 and DRB110 are associated with resistance and susceptibility, respectively, in Brazilian and Vietnamese leprosy patients

et al. 2007

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The host genetic background has been considered one of the factors that influence leprosy outcome, a chronic infectious disease caused by Mycobacterium leprae. Genome scans demonstrated that the 6p21 region is associated with leprosy and a substantial number of population-based studies analyzing human leukocyte antigen (HLA) class II loci suggested association of HLA-DR with leprosy. However, some studies lacked robustness as they had limited power. Indeed, experimental designs require increased sample size to achieve adequate power, as well as replication studies with independent samples for confirmation of previous findings. In this work, we analyzed the influence of the HLA-DRB1 locus on leprosy susceptibility per se and disease type using a case-control design carried out in Brazilians (578 cases and 691 controls) and a replication study based on a family design in a Vietnamese population (n ¼ 194 families). The results showed that HLA-DRB1*10 is associated with susceptibility to leprosy and HLA-DRB1*04 is associated with resistance, both in the Brazilian and Vietnamese populations suggesting that these alleles play an important role in the activation of cellular immune responses against M. leprae.

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HLA Class-II Immune Response Genes and Products in Leprosy

Cited by 15 publications

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Epstein‐Barr Virus‐Transformed B Cell Lines Present M. Leprae Antigens to T Cells

Epstein‐Barr Virus‐Transformed B Cell Lines Present M. Leprae Antigens to T Cells

Cellular and Humoral Immune Responses of Cattle to Purified Mycobacterium bovis Antigens

HLA-DRB104 and DRB110 are associated with resistance and susceptibility, respectively, in Brazilian and Vietnamese leprosy patients

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HLA Class-II Immune Response Genes and Products in Leprosy

Cited by 15 publications

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Epstein‐Barr Virus‐Transformed B Cell Lines Present M. Leprae Antigens to T Cells

Epstein‐Barr Virus‐Transformed B Cell Lines Present M. Leprae Antigens to T Cells

Cellular and Humoral Immune Responses of Cattle to Purified Mycobacterium bovis Antigens

HLA-DRB1*04 and DRB1*10 are associated with resistance and susceptibility, respectively, in Brazilian and Vietnamese leprosy patients

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Product

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HLA-DRB104 and DRB110 are associated with resistance and susceptibility, respectively, in Brazilian and Vietnamese leprosy patients