HLA Class II Allele, Haplotype, and Genotype Associations with Type 1 Diabetes in Benin: A Pilot Study

Fagbemi, Kaossarath A.; Medehouenou, Thierry Comlan Marc; Azonbakin, Simon; Adjagba, Marius; Osseni, Razack; Ahoueya, Jocelyne; Agbanlinsou, Arnaud; Darboux, R.; Baba-Moussa, Lamine; Laleye, Anatóle

doi:10.1155/2017/6053764

Cited by 5 publications

(3 citation statements)

References 27 publications

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“…Some common alleles at HLA Class II loci (HLA‐DRB1, ‐DQA1, and ‐DQB1) are primarily and jointly associated with the disease, as shown by a large number of case‐control and family‐based association studies from different populations . These three loci have both haplotype‐ and genotype‐specific effects and act as a complex superlocus .…”

Section: Introductionmentioning

confidence: 99%

Diverse human leukocyte antigen association of type 1 diabetes in north India

Kumar

Mehra

Kanga

et al. 2019

Journal of Diabetes

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Highlights• Type 1 diabetes (T1D) in north India is strongly associated with HLA-DRB1*03 haplotypes. • The association between HLA-DRB1*04 and T1D in north India differs from that in south India and in Caucasians. AbstractBackground: Type 1 diabetes (T1D) is a complex disease, with involvement of various susceptibility genes. Human leukocyte antigen (HLA) on chromosome 6p21 is major susceptibility region. This study examined genetic association of HLA genes with T1D.Methods: The study recruited 259 T1D patients and 706 controls from north India. PCR-SSP and LiPA were used to type HLA Class I and II alleles. Results: At HLA Class I locus, HLA-A*02, A*26, B*08 and B*50 were significantly increased in patients vs controls (39.8% vs 28.9% [Bonferroni-corrected P {P c } = 0.032], 24.7% vs 9.6% [P c = 4.83 × 10 −8 ], 37.2% vs 15.7% [P c = 1.92 × 10 −9 ], and 19.4% vs 5.5% [P c = 4.62 × 10 −9 ], respectively). Similarly, in Class II region, DRB1*03 showed a strong positive association with T1D (78.7% vs 17.5% in controls; P = 1.02 × 10 −9 ). Association of DRB1*04 with T1D (28.3% vs 15.5% in controls; P c = 3.86 × 10 −4 ) was not independent of DRB1*03. Negative associations were found between T1D and DRB1*07, *11, *13, and *15 (13.8% vs 26.1% in controls [P c = 0.00175], 3.9% vs 16.9% in controls [P c = 6.55× 10 −6 ], 5.5% vs 21.6% in controls [P c = 2.51 × 10 −7 ], and 16.9% vs 43.9% in controls [P c = 9.94× 10 −10 ], respectively). Compared with controls, patients had significantly higher haplotype frequencies of A

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Section: Introductionmentioning

confidence: 99%

Diverse human leukocyte antigen association of type 1 diabetes in north India

Kumar

Mehra

Kanga

et al. 2019

Journal of Diabetes

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“…HLA -DQA105:01 and HLA-DR3 genotyping was performed with PCR-sequence-specific primers (PCR-SSP) . 11 . In a 20 μl mixture of 2.5 µl DNA, 1.5 µl from each forward and reverse of the primer, 5 µl master mix and 9.5 µl nuclease free water.…”

Section: Hla Genotype Analysismentioning

confidence: 99%

“…In a 20 μl mixture of 2.5 µl DNA, 1.5 µl from each forward and reverse of the primer, 5 µl master mix and 9.5 µl nuclease free water. The DNA amplification for HLA-DQA1 is includes an initial denaturation of 2 min in 94°C, 32 cycles of amplification (every cycle consists of a denaturation of 30 s in 94°C, a hybridization of primers during 30 s in 63°C, and an extension of 30 s in 72°C), and a final extension of 10 min in72°C, 11 . While the process of DNA amplification for HLA-DR3 is same as DQA1, (exception the hybridization temperature was 52°C), then the PCR products were separated in 1.5 % agarose electrophoresis system using ethidium bromide then visualized with the gel documentation, with 100 bp-ladder (Bioneer, Korea) and photographed.…”

Section: Hla Genotype Analysismentioning

confidence: 99%

Genotype and Haplotype of HLA- Class II in Type I and Type II Diabetes Mellitus in Iraqi Patients

2020

IJFMT

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Background: Diabetes is one of the common complicated disease associated with multiple influenced factors, immunological serological and molecular factors play a great role in susceptibility and diagnosis of this disease. HLA genotyping were reported to have an influencing in both types of diabetes. Aim:The current study was conducted to investigate the association of human leukocytes genotypes in Diabetes patients.Method: Case-control study enrolled 225 blood samples collected from patient attended to the Marjan Teaching Hospital-Hilla and 25 apparently healthy from October 2018 to May 2019. Class II HLA genotyping was performing for 60 patients with diabetes mellitus and 25 healthy unrelated controls by means of the PCR-SSP method. The diagnosis of T1D and T1ID was set up according to American Diabetes Association criteria.Results: both types of diabetes were significantly associated with HLA-DR3. Associations were also observed with HLA -DQA105:01, the appearance of these two alleles differs in both TID and TIID . Conclusion. Certain HLA class II alleles, haplotypes, and genotypes have related to diabetes mellitus so it can be dependent as a genetic marker for susceptibility of this disease in Iraq.

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Genetic profile considerations for induction of allogeneic chimerism as a therapeutic approach for type 1 diabetes mellitus

Black

Zorina

2020

Drug Discovery Today

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HLA Class II Allele, Haplotype, and Genotype Associations with Type 1 Diabetes in Benin: A Pilot Study

Cited by 5 publications

References 27 publications

Diverse human leukocyte antigen association of type 1 diabetes in north India

Diverse human leukocyte antigen association of type 1 diabetes in north India

Genotype and Haplotype of HLA- Class II in Type I and Type II Diabetes Mellitus in Iraqi Patients

Genetic profile considerations for induction of allogeneic chimerism as a therapeutic approach for type 1 diabetes mellitus

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