HKGreen-3: A Rhodol-Based Fluorescent Probe for Peroxynitrite

Tao, Ping; Yang, Dan

doi:10.1021/ol102182j

Cited by 137 publications

(82 citation statements)

References 17 publications

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“…HKGreen-1 was developed based on this reaction design and can image changes in ONOO − levels in primary cultured neuronal cells 38 . Next-generation versions give improved turn-on responses and can visualize endogenous ONOO − fluxes produced upon immune stimulation 39,40 .…”

Section: Organic Reaction-based Approachesmentioning

confidence: 99%

Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

2012

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The dynamic chemical diversity of elements, ions and molecules that form the basis of life offers both a challenge and an opportunity for study. Small-molecule fluorescent probes can make use of selective, bioorthogonal chemistries to report on specific analytes in cells and in more complex biological specimens. These probes offer powerful reagents to interrogate the physiology and pathology of reactive chemical species in their native environments with minimal perturbation to living systems. This Review presents a survey of tools and tactics for using such probes to detect biologically important chemical analytes. We highlight design criteria for effective chemical tools for use in biological applications as well as gaps for future exploration.

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Section: Organic Reaction-based Approachesmentioning

confidence: 99%

Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

2012

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“…HKGreen-3, a rhodol-based fluorescent probe, is recently developed by Peng et al and shows high sensitivity and selectivity for peroxynitrite in both chemical and biological systems [60]. HKOCl-1 is a BODIPY-based fluorescent probe for detecting hypochlorous acid with high specificity [61].…”

Section: Ros Measurement With Small-molecule Fluorescent Probesmentioning

confidence: 99%

Imaging ROS signaling in cells and animals

et al. 2013

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Reactive oxygen species (ROS) act as essential cellular messengers, redox regulators, and, when in excess, oxidative stressors that are widely implicated in pathologies of cancer and cardiovascular and neurodegenerative diseases. Understanding such complexity of the ROS signaling is critically hinged on the ability to visualize and quantify local, compartmental, and global ROS dynamics at high selectivity, sensitivity, and spatiotemporal resolution. The past decade has witnessed significant progress in ROS imaging at levels of intact cells, whole organs or tissues, and even live organisms. In particular, major advances include the development of novel synthetic or genetically encoded fluorescent protein-based ROS indicators, the use of protein indicator-expressing animal models, and the advent of in vivo imaging technology. Innovative ROS imaging has led to important discoveries in ROS signaling—for example, mitochondrial superoxide flashes as elemental ROS signaling events and hydrogen peroxide transients for wound healing. This review aims at providing an update of the current status in ROS imaging, while identifying areas of insufficient knowledge and highlighting emerging research directions.

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“…With this probe, we discovered endogenous ONOO -generation in oxygen-glucose-deprived cortical neurons [128] . Based on the first generation of HKGreen-1, several modified probes have been produced [129,130] . These modified probes aim to act as peroxynitrite scavengers with the potential to protect ischemic brains from the ONOO --mediated injury.…”

Section: Development Of Fluorescent Probes For Peroxynitrite Detectionmentioning

confidence: 99%

Targeting reactive nitrogen species: a promising therapeutic strategy for cerebral ischemia-reperfusion injury

Chen

et al. 2012

Acta Pharmacol Sin

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Ischemic stroke accounts for nearly 80% of stroke cases. Recanalization with thrombolysis is a currently crucial therapeutic strategy for re-building blood supply, but the thrombolytic therapy often companies with cerebral ischemia-reperfusion injury, which are mediated by free radicals. As an important component of free radicals, reactive nitrogen species (RNS), including nitric oxide (NO) and peroxynitrite (ONOO -), play important roles in the process of cerebral ischemia-reperfusion injury. Ischemia-reperfusion results in the production of nitric oxide (NO) and peroxynitrite (ONOO -) in ischemic brain, which trigger numerous molecular cascades and lead to disruption of the blood brain barrier and exacerbate brain damage. There are few therapeutic strategies available for saving ischemic brains and preventing the subsequent brain damage. Recent evidence suggests that RNS could be a therapeutic target for the treatment of cerebral ischemia-reperfusion injury. Herein, we reviewed the recent progress regarding the roles of RNS in the process of cerebral ischemic-reperfusion injury and discussed the potentials of drug development that target NO and ONOO -to treat ischemic stroke. We conclude that modulation for RNS level could be an important therapeutic strategy for preventing cerebral ischemiareperfusion injury.

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HKGreen-3: A Rhodol-Based Fluorescent Probe for Peroxynitrite

Abstract: A novel fluorescent probe, HKGreen-3, for sensing peroxynitrite is designed on the basis of the rhodol scaffold and a peroxynitrite-specific oxidation reaction. The probe turns out to be highly sensitive and selective for detecting peroxynitrite in both chemical and biological systems.

Cited by 137 publications

References 17 publications

Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

Reaction-based small-molecule fluorescent probes for chemoselective bioimaging

Imaging ROS signaling in cells and animals

Targeting reactive nitrogen species: a promising therapeutic strategy for cerebral ischemia-reperfusion injury

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