HIV test result disclosure

Wiwanitkit, Viroj

doi:10.1258/ijsa.2009.008500

Cited by 1 publication

(1 citation statement)

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“…For example, Curtis et al (2012) designed a noninstrumented nucleic acid heater for point-of-care detection of HIV-1, and the LAMP assay for detection of E. coli has been conducted using a self-heating cartridge (Liu et al, 2011). Additionally, the analysis of on-site detection revealed that LAMP products integrating with these electricityfree devices could be simultaneously detected by immunochromatographic strip and fluorescent dyes to obtain visual detection results (Hatano et al, 2010;Singleton et al, 2014). However, all aforementioned devices lack the capabilities of bacteria pre-enrichment and DNA extraction, despite various DNA extraction methods that have been developed (Abdelhai et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Electricity-free amplification and visual detection of Cronobacter species in powdered infant formula

Fu¹,

Jiang²,

Xue

et al. 2020

Journal of Dairy Science

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Due to the lack of electricity and thermostatic instruments in certain settings for convenient detection of Cronobacter species in powdered infant formula (PIF), a novel investigation was conducted to establish an electricity-free visual detection system for rapid detection of Cronobacter species in PIF. This system included a portable electricity-free heater that could use the exothermic reaction of calcium oxide and water and 3 kinds of phase change materials to supply 3 constant temperatures for immunomagnetic separation, DNA extraction, and loop-mediated isothermal amplification assay. Meanwhile, the amplified reaction combined with hydroxynaphthol blue could achieve rapid visual detection. Primers designed based on the 16S-23S ribosomal RNA internal transcribed spacer were used in loop-mediated isothermal amplification to specifically monitor Cronobacter species, and the detection limit can reach 4.2 × 10 2 cfu/g in PIF by an electricity-free heater in 2 h 30 min. Moreover, 2 h of pre-enrichment was necessary when the level of the PIF samples with Cronobacter spp. was 10 0 cfu/g. The stability of the system was evaluated in ambient temperature at 4°C, 25°C, and 37°C. The results suggested that the electricity-free heater can maintain 3 constant temperatures to support different processes. Therefore, this amplification and visual system is applicable for use in many fields for rapid and specific detection of Cronobacter species in PIF.

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