HIV seroprevalence and HIV-1 subtype E among pregnant women in Vientiane, Laos

The hypervariable V3 loop within gp120 of human immunodeficiency virus type 1 (HIV-1) is the major determinant of cell tropism and the entry coreceptor usage of the virus. However, the information obtained thus far has been from only subtype B from North America and Europe, and little is known about other subtypes whose V3 amino acids differ by as much as 50% from subtype B V3. In this study, we examined the functional potential of the V3 element of the HIV-1 subtype E, the most crucial variant causing the AIDS epidemic throughout southeast Asia. A panel of HIV-1LAI recombinants was constructed by the overlap extension method, by which the LAI V3 loop was precisely replaced by that of the subtype E nonsyncytium-inducing (NSI) or syncytium-inducing (SI) variant. All of the recombinant viruses infected peripheral blood mononuclear cells, whereas only those with SI V3 infected MT2 cells, a CD4(+) T cell line. Consistently, the SI V3 recombinants used CXCR4, while the NSI V3 recombinants used CCR5 for infection of HOS-CD4(+) cells. Finally, only the NSI V3 sequence conferred CC-chemokine sensitivity on the parental virus. The data support the notion that the HIV-1 V3 loop consists of a relatively independent domain in gp120 and suggest that the subtype E V3 loop indeed contains the functional element to dictate the cell tropism, coreceptor preference, and chemokine sensitivity of the virus. These findings are of immediate importance in understanding V3 structure-function relationship and for examining phenotypic evolution of HIV-1 subtype E.

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Functional Complementation of the Envelope Hypervariable V3 Loop of Human Immunodeficiency Virus Type 1 Subtype B by the Subtype E V3 Loop

Sato

Kato

Takebe

1999

Virology

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Laos is Affected by HIV CRF01_AE and the Newly Identified CRF97_01B

Chen

Wang

et al. 2020

Virol. Sin.

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Dual group screening

Johnson

Gastwirth

2000

Journal of Statistical Planning and Inference

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HIV seroprevalence and HIV-1 subtype E among pregnant women in Vientiane, Laos

Cited by 6 publications

References 1 publication

Functional Complementation of the Envelope Hypervariable V3 Loop of Human Immunodeficiency Virus Type 1 Subtype B by the Subtype E V3 Loop

Functional Complementation of the Envelope Hypervariable V3 Loop of Human Immunodeficiency Virus Type 1 Subtype B by the Subtype E V3 Loop

Laos is Affected by HIV CRF01_AE and the Newly Identified CRF97_01B

Dual group screening

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