HIV-2 genomic RNA accumulates in stress granules in the absence of active translation

Soto-Rifo, Ricardo; Valiente-Echeverría, Fernando; Rubilar, Paulina S.; García-de-Gracia, Francisco; Ricci, Emiliano P.; Limousin, Taran; Décimo, Didier; Mouland, Andrew J.; Ohlmann, Théophile

doi:10.1093/nar/gku1017

Cited by 15 publications

(22 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Assembly Of Unspliced Mrna-containing Granulescontrasting

confidence: 99%

“…In sharp contrast with what was described for HIV-1, we showed that HIV-2 replication induces the spontaneous assembly of stress granules [ 166 ] ( Figure 3 B). Moreover, we observed that HIV-2 unspliced mRNA was directly associated with the stress granule assembly factor TIAR in order to form a specific viral mRNP [ 166 ] ( Figure 3 B). We have previously shown that ribosome recruitment onto the HIV-2 genomic RNA is very inefficient due to a strong interference imposed by the highly structured TAR RNA motif [ 109 ].…”

Section: Assembly Of Unspliced Mrna-containing Granulescontrasting

confidence: 99%

“…Thus, stress granules could serve as sites of storage for the viral genome while threshold levels of Gag required for RNA packaging are produced. Interestingly, the HIV-2 Gag polyprotein was also observed in stress granules indicating that the transition from translation to RNA packaging may occur in these structures [ 166 ] ( Figure 3 B).…”

Section: Assembly Of Unspliced Mrna-containing Granulesmentioning

confidence: 99%

See 2 more Smart Citations

Translational Control of the HIV Unspliced Genomic RNA

Rojas-Araya

Ohlmann

Soto-Rifo

2015

Viruses

Self Cite

View full text Add to dashboard Cite

Post-transcriptional control in both HIV-1 and HIV-2 is a highly regulated process that commences in the nucleus of the host infected cell and finishes by the expression of viral proteins in the cytoplasm. Expression of the unspliced genomic RNA is particularly controlled at the level of RNA splicing, export, and translation. It appears increasingly obvious that all these steps are interconnected and they result in the building of a viral ribonucleoprotein complex (RNP) that must be efficiently translated in the cytosolic compartment. This review summarizes our knowledge about the genesis, localization, and expression of this viral RNP.

show abstract

Section: Assembly Of Unspliced Mrna-containing Granulescontrasting

confidence: 99%

Section: Assembly Of Unspliced Mrna-containing Granulescontrasting

confidence: 99%

Section: Assembly Of Unspliced Mrna-containing Granulesmentioning

confidence: 99%

See 1 more Smart Citation

Translational Control of the HIV Unspliced Genomic RNA

Rojas-Araya

Ohlmann

Soto-Rifo

2015

Viruses

Self Cite

View full text Add to dashboard Cite

show abstract

“…While not all mRNAs are destined to enter stress granules , cellular mRNAs are not the exclusive RNA components of stress granules. For instance, HIV‐2 and human respiratory syncytial virus (hRSV) genomic RNAs have been shown to reside in stress granules following infection .…”

Section: Mrnas Inside Stress Granulesmentioning

confidence: 99%

mRNPs meet stress granules

Sheinberger

Shav‐Tal

2017

FEBS Letters

View full text Add to dashboard Cite

Edited by Wilhelm JustStress granules are cytoplasmic structures that form in response to a variety of cellular stresses. They contain mRNAs and many proteins including numerous types of RNA-binding proteins, and have been studied in connection to major cellular events such as protein synthesis as well as disease. Despite the well-known fact that stress granules encapsulate mRNPs (mRNA-protein complexes), much of the research has naturally focused on the protein components of stress granules. The specific details of mRNP entry into and exit from stress granules and the functional reasons for these dynamics are not fully understood. Here, we review studies that have concentrated on the aspects of mRNP accumulation in stress granules and produced quantitative data concerning mRNP/stress granule interactions.

show abstract

“…In this context, we speculate that all successful retroviruses and endogenous elements must adapt to subvert, suppress, or avoid PB- or SG-associated activities. Indeed, HIV-1 42,43 , other retroviruses 44,45 , and endogenous retroelements including LINEs 46 and yeast Ty elements 47 are thought to exploit non-membranous RNP complexes related to PBs/SGs in order to compartmentalize activities including mRNA translation and genome packaging. Regarding suppression, intriguing recent work from Mouland and colleagues has shown HIV-1 to actively inhibit SG formation, proposed to reduce the potential for stress signaling triggered by Gag NC interacting with host RNA species 33,34 .…”

Section: Discussionmentioning

confidence: 99%

HIV-1 RNA genomes initiate host protein packaging in the cytosol independently of Gag capsid proteins

Becker

Evans

Benner

et al. 2019

Preprint

View full text Add to dashboard Cite

17HIV-1 RNA genomes interact with diverse RNA binding proteins in the cytoplasm 18 including antiviral factor APOBEC3G (A3G) that, in the absence of viral Vif proteins, is 19 packaged into virions. When and where genome-A3G interactions are initiated in the host 20 cell is unknown. Here we use quantitative long-term (>24 h) live cell fluorescence video 21 microscopy and a new in-cell RNA-protein interaction assay (the "IC-IP") to describe 22 subcellular viral and A3G trafficking behaviors over the entire HIV-1 productive phase. 23Among other findings, we demonstrate that genome-A3G interactions are initiated in the 24 cytosol soon if not immediately after genome nuclear export; that A3G-genome 25 interactions are sufficiently strong so that tethering either factor to membranes inhibits 26 trafficking of the reciprocal binding partner; and that selective recognition of genomes 27 promotes consistent delivery of A3G to sites of virion assembly. Further elucidation of 28 RNA signature(s) detected by A3G may inform development of RNA-targeted antivirals. 29 RNA-and NC-dependent15-20. However, the relative contributions of genomes vs. host 50RNAs to this process remains controversial. On the one hand, A3G-RNA binding is 51 relatively promiscuous, with A3G incorporated into virus particles even when packageable 52 genomes are not expressed17,19. On the other hand, A3G incorporation levels are 53 moderately enhanced when genomes are present16,21; and A3G has been shown to 54 exhibit selective RNA-binding characteristics19,22 including a reported preference for G-55 rich segments of the HIV-1 genome20. 56Where and when A3G interfaces with Gag and/or genomes in the cell has also been 57 under investigation for some time with conflicting results. At steady-state, A3G is 58 distributed throughout the cytosol (the aqueous phase of the cytoplasm) and accumulates 59 to high levels at non-membranous cytoplasmic sites of mRNA decay known as processing 60 bodies (PBs)23,24. Cytosolic A3G is also rapidly re-localized to sites of translational 61 repression known as stress granules (SGs) in response to heat shock or oxidative 62 stress23,24. Initial studies proposed a functional link between PBs and A3G's antiviral 63 activity23,25,26. By contrast, more recent work has shown visible PBs to have little to no 64 discernible relevance to the HIV-1 life cycle27,28. 65The dynamic and complex nature of cytoplasmic RNA trafficking during HIV-1 virion 66 genesis may have obscured consistent prior observations made in fixed cells, 67 emphasizing a need for real-time characterization the host RBP response to HIV-1 68 infection at subcellular resolution. Accordingly, herein we set out to comprehensively 69 define the behaviors of HIV-1 RNA genomes, Gag, A3G, and additional PB and SG 70 markers over the entire viral productive phase using long-term (>24h) live cell video 71 microscopy. Our results expose single-cell A3G degradation and trafficking behaviors in 72 the presence or absence of Vif; and show that HIV-1 infection has little to no ne...

show abstract

HIV-2 genomic RNA accumulates in stress granules in the absence of active translation

Cited by 15 publications

References 56 publications

Translational Control of the HIV Unspliced Genomic RNA

Translational Control of the HIV Unspliced Genomic RNA

mRNPs meet stress granules

HIV-1 RNA genomes initiate host protein packaging in the cytosol independently of Gag capsid proteins

Contact Info

Product

Resources

About