HIV‐1 viral load testing in resource‐limited settings: Challenges and solutions for specimen integrity

Newman, Howard; Hardie, Diana

doi:10.1002/rmv.2165

Cited by 15 publications

(19 citation statements)

References 34 publications

(117 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The possible application of this finding depends on the ongoing roll out of viral load monitoring in line with the WHO guidelines and is still limited in these settings. 17 The analyses also showed that a longer duration of HIV infection was associated with both a higher risk of TB (Table 2) and other non-TB AIDS events. Despite ART treatment, the duration of HIV infection should be considered in the clinical management of this population who continue to experience a greater risk of AIDS-related morbidity.…”

Section: Discussionmentioning

confidence: 87%

Factors Associated With and Characteristic of HIV/Tuberculosis Co-Infection: A Retrospective Analysis of SECOND-LINE Clinical Trial Participants

Henry

Jiamsakul

Law

et al. 2021

JAIDS Journal of Acquired Immune Deficiency Syndromes

View full text Add to dashboard Cite

Background: Tuberculosis (TB) is a common infection in people living with HIV. However, the risk factors for HIV/TB co-infection in second-line HIV therapy are poorly understood. We aimed to determine the incidence and risk factors for TB co-infection in SECOND-LINE, an international randomized clinical trial of second-line HIV therapy.Methods: We did a cohort analysis of TB cases in SECOND-LINE. TB cases included any clinical or laboratory-confirmed diagnoses and/or commencement of treatment for TB after randomization. Baseline factors associated with TB were analyzed using Cox regression stratified by site.Results: TB cases occurred at sites in Argentina, India, Malaysia, Nigeria, South Africa, and Thailand, in a cohort of 355 of the 541 SECOND-LINE participants. Overall, 20 cases of TB occurred, an incidence rate of 3.4 per 100 person-years (95% CI: 2.1 to 5.1). Increased TB risk was associated with a low CD4 + -cell count (#200 cells/mL), high viral load (.200 copies/mL), low platelet count (,150 •10 9 /L), and low total serum cholesterol (#4.5 mmol/L) at baseline. An increased risk of death was associated with TB, adjusted for CD4, platelets, and cholesterol. A low CD4 + -cell count was significantly associated with incident TB, mortality, other AIDS diagnoses, and virologic failure.Discussion: The risk of TB remains elevated in PLHIV in the setting of second-line HIV therapy in TB endemic regions. TB was associated with a greater risk of death. Finding that low CD4 + T-cell count was significantly associated with poor outcomes in this population supports the value of CD4 + monitoring in HIV clinical management.

show abstract

Section: Discussionmentioning

confidence: 87%

Factors Associated With and Characteristic of HIV/Tuberculosis Co-Infection: A Retrospective Analysis of SECOND-LINE Clinical Trial Participants

Henry

Jiamsakul

Law

et al. 2021

JAIDS Journal of Acquired Immune Deficiency Syndromes

View full text Add to dashboard Cite

show abstract

“… 12 The stability of PSC specimens stored at high temperatures for up to 28 days is an important feature for field conditions in resource-limited settings, given that in most sub-Saharan African countries, preshipment storage at health facilities and transportation of specimens to the reference laboratories can take up to a month. 16 …”

Section: Discussionmentioning

confidence: 99%

Stability of HIV-1 Nucleic Acids in cobas Plasma Separation Card for Viral Load Measurement

Vubil

Nhachigule

Zicai

et al. 2022

American Journal of Clinical Pathology

View full text Add to dashboard Cite

Objectives Our study aimed to evaluate the stability of human immunodeficiency virus 1 (HIV-1) RNA on cobas plasma separation card (PSC) specimens for viral load (VL) testing after being exposed to varied temperatures and storage times. Methods For this purpose, venous PSC specimens were collected and stored at 25ºC to 42ºC for a period of up to 28 days. Plasma VL at baseline was used as reference, against which PSC VL was compared at different time points. Results From the 30 patients included in the study, 600 PSC and 30 fresh plasma specimens were obtained. Plasma VL at baseline was fewer than 1,000 copies/mL in 16 patients, and 99.4% of PSCs from these patients yielded nonquantifiable VL at all temperature ranges and time points. During the study period, minor variation of VL was observed in PSCs obtained from 13 patients with plasma VL fewer than 1,000 copies/mL at baseline. For the patient with plasma VL at 1,000 copies/mL, the PSC VL varied from undetectable to 1,670 copies/mL. Conclusions Our results show minor variation of VL in PSC specimens in the study conditions. HIV RNA is stable in PSCs exposed to high temperatures for up to 28 days.

show abstract

“…10,32 As in other countries in sub-Saharan Africa, fidelity to the recommended VL testing schedules was challenging in Nigeria, resulting in missed measures. [33][34][35] VL testing was completed at reference laboratories after whole blood sample collection at the facilities using Roche COBAS AmpliPrep/ TaqMan HIV-1 Test. For the primary analyses, which used only routine patient data, we included participants with .1 VL measure in the study period.…”

Section: Analysis Measures and Definition Of Variablesmentioning

confidence: 99%

Effect of Dolutegravir and Multimonth Dispensing on Viral Suppression Among Children With HIV

Mugo

Zubayr²,

Ezeokafor³

et al. 2023

JAIDS Journal of Acquired Immune Deficiency Syndromes

View full text Add to dashboard Cite

show abstract

HIV‐1 viral load testing in resource‐limited settings: Challenges and solutions for specimen integrity

Cited by 15 publications

References 34 publications

Factors Associated With and Characteristic of HIV/Tuberculosis Co-Infection: A Retrospective Analysis of SECOND-LINE Clinical Trial Participants

Factors Associated With and Characteristic of HIV/Tuberculosis Co-Infection: A Retrospective Analysis of SECOND-LINE Clinical Trial Participants

Stability of HIV-1 Nucleic Acids in cobas Plasma Separation Card for Viral Load Measurement

Effect of Dolutegravir and Multimonth Dispensing on Viral Suppression Among Children With HIV

Contact Info

Product

Resources

About