HIV-1 Tat biosensor: Current development and trends for early detection strategies

Fatin, M. F.; Ruslinda, A. Rahim; Arshad, M. K. Md; Tee, Kok Keng; Ayub, R. M.; Hashim, U.; Kamarulzaman, Adeeba; Gopinath, Subash C. B.

doi:10.1016/j.bios.2015.11.067

Cited by 23 publications

(6 citation statements)

References 62 publications

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“…HIV accessory proteins include the trans-activator of transcription (Tat) protein, which is important for the transcription of viral RNA and enhances the amount of protein produced by attaching itself to the viral RNA. 72 The Tat protein is one of the most promising candidates for HIV screening because this protein is released in the body at an early stage of infection. 72 Yamamoto et al .…”

Section: Hiv-1mentioning

confidence: 99%

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Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Pan

Liu

Zhang

2018

Journal of Infection

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The entire human population is at risk of infectious diseases worldwide. Thus far, the diagnosis and treatment of human infectious diseases at the molecular and nanoscale levels have been extremely challenging tasks because of the lack of effective probes to identify and recognize biomarkers of pathogens. Oligonucleotide aptamers are a class of small nucleic acid ligands that are composed of single-stranded DNA (ssDNA) or RNA and act as affinity probes or molecular recognition elements for a variety of targets. These aptamers have an exciting potential for diagnose and/or treatment of specific diseases. In this review, we highlight areas where aptamers have been developed as diagnostic and therapeutic agents for both bacterial and viral infectious diseases as well as aptamer-based detection.

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Section: Hiv-1mentioning

confidence: 99%

“…72 The Tat protein is one of the most promising candidates for HIV screening because this protein is released in the body at an early stage of infection. 72 Yamamoto et al . first generated an RNA aptamer specific for the HIV-1 Tat protein.…”

Section: Hiv-1mentioning

confidence: 99%

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Pan

Liu

Zhang

2018

Journal of Infection

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“…HIV infection, therefore, still is a major public health issue in the world; moreover, HIV testing is not accessible to everyone. There are two types of HIVs: HIV-1 and HIV-2, but research on HIV mostly focuses on HIV-1 since it is more viral and pandemic (Fatin et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

An electrochemical label-free DNA impedimetric sensor with AuNP-modified glass fiber/carbonaceous electrode for the detection of HIV-1 DNA

et al. 2020

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In this study, a highly sensitive, electrochemical, and label-free DNA impedimetric sensor was developed using carbonized glass fiber-coal tar pitch (GF-CTP) electrodes supported with gold nanoparticles (AuNPs) for the detection of HIV-1 gene. Thiol-modified GF-CTP electrodes were prepared using amine crosslinking chemistry and AuNPs were self-assembled obtaining highly conductive nanoelectrodes, GF-CTP-ATP-Au. All steps of electrode modifications were characterized using electrochemical, spectroscopic, and microscopic methods. GF-CTP-ATP-Au electrode was then modified with a capture DNA probe (C-ssDNA) and optimized with a target DNA probe in terms of hybridization time and temperature between 30 and 180 min and 20-50 °C, respectively. Finally, the analytic performance of the developed ssDNA biosensor was evaluated using electrochemical impedance spectroscopy. The calibration of the sensor was obtained between 0.1 pM and 10 nM analyte working range. The limit of detection was calculated using signal to noise ratio of 3 (S/N = 3) as 13 fM. Moreover, interference results for two noncomplementary DNA probes were also tested to demonstrate non-specific ssDNA interactions. An electrochemical label-free DNA impedimetric sensor was successfully developed using a novel GF-CTP-ATP-Au electrode. This study suggests that highly sensitive DNA-based biosensors can be developed using relatively lowcost carbonaceous materials.

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“…The main HIV subtypes are referred to as HIV-1 and HIV-2, but studies on HIV mostly focus on HIV-1 which is more fatal and transmissible. 4 The vital target for the treatment of HIV virus is reverse transcriptase because of its major role in virus multiplication. 5 HIV viruses use the host cell's machinery to replicate.…”

mentioning

confidence: 99%

Review—Nanostructured Electrochemical Sensors for Determination of the First Generation of the NNRTIs for HIV-1

Silwana

Matoetoe²

2022

ECS Adv.

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Since the 1980s, the HIV/AIDS pandemic has been a global concern and remains despite the tremendous efforts and successes in the development of antiviral drugs. The drug development and formulation process are not without challenges. However, the effective use of excipients can allow formulators to circumvent or at least minimize the impact of these challenges. Some of the challenges include ensuring a high quality of actives, free from impurities, and ensuring administration in appropriate quantities. Incorrect dosing can have a detrimental impact on the body. Evaluation of the quality and physiological function of these drugs in pharmaceutical and biological samples is vital. We explore the application of electrochemical methods for the detection of these drugs. Electrochemical methods are versatile and cheaper in comparison to conventional techniques. This review paper provides the latest developments and new trends in electroanalysis of the first generation of nonnucleoside reverse transcriptase inhibitors drugs. Furthermore, we highlight several phenomena occurring on bare solid electrodes and modified electrodes, including underlying mechanisms. Recent data shows that modified electrodes based on nanoparticles present substantial advantages over their non-modified counterparts enabling portability, higher selectivity, and multiple detection capabilities. These characteristics make nanoparticles promising candidates for the design of sensors.

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HIV-1 Tat biosensor: Current development and trends for early detection strategies

Cited by 23 publications

References 62 publications

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases

An electrochemical label-free DNA impedimetric sensor with AuNP-modified glass fiber/carbonaceous electrode for the detection of HIV-1 DNA

Review—Nanostructured Electrochemical Sensors for Determination of the First Generation of the NNRTIs for HIV-1

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