Since 1985 the Institutes of Forensic Medicine in Germany and in several European cities have cooperated in a multicenter study to monitor HIV-1 prevalence among drug abuse-related deaths. Data for 1993 are presented from Berlin, Frankfurt, Hamburg, Munich, Bilbao, Coimbra, Copenhagen, Edinburgh, Helsinki, Madrid, Oslo, Rome, Stockholm, Vienna and Zurich. Regional differences are obvious. HIV-1 prevalence rates are low in Scandinavia (Helsinki 0%, Oslo 6.1 %, Stockholm 6.4%, Copenhagen 6.6%) and comparatively high in the Mediterranean area (Bilbao 79%, Madrid 53.6%, Coimbra 37.5% and Rome 27.3%). The prevalence rate seems to be stabilizing in German cities at a rate of about 5–15%.