HIV-1 integrase-hydrolyzing antibodies from sera of HIV-infected patients

Баранова, Светлана Владимировна; Buneva, Valentina N.; Kharitonova, M. A.; Sizyakinа, L. P.; Calmels, Christina; Andréola, Marie‐Line; Parissi, Vincent; Nevinsky, Georgy A.

doi:10.1016/j.biochi.2009.06.018

Cited by 48 publications

(169 citation statements)

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“…However, iodoacetamide inhibited integrase hydrolyzing activities of all polyclonal IgG and IgM preparations from HIV/AIDS patients by 12-99% [97,98]. Proteolytic activities of three MLChs (18, 14, and 10) not inhibited by EDTA and PMSF were significantly suppressed by iodoacetamide, while there was no effect on the most of MLChs with metal-dependent and serinelike activities (for example, Figure 20C).…”

Section: Catalytic Antibodies In Norm and Systemic Lupus Erythematosusmentioning

confidence: 96%

“…In addition, a high metal-dependent MBP-hydrolyzing activity for MS IgGs [86] and caseinhydrolyzing of human milk sIgAs [96] were recently revealed. It was shown that antiintegrase IgGs and IgMs of HIV-infected patients can contain abzymes hydrolyzing viral integrase of four types, resembling serine, thiol, metal-dependent, and acidic proteases, the ratio of which may be individual for every AIDS patient [97,98].…”

Section: Lupus 70mentioning

confidence: 99%

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Catalytic Antibodies in Norm and Systemic Lupus Erythematosus

Nevinsky¹

2017

Lupus

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Systemic lupus erythematosus (SLE) is known as a systemic polyethiologic diffuse autoimmune disease characterized by connective tissue disorganization and the paramount damage of skin and visceral capillaries. Usually, SLE symptoms include high fever, hair loss, mouth ulcers, chest pain, swollen lymph nodes, painful and swollen joints, increased fatigue, and appearance of red rash more often on the face. The exact reason of SLE appearance is not really clear. Detection of catalytic Abs (abzymes) was shown to be the earliest indicator of different AI disease development. Some abzymes are cytotoxic and can play a dangerous negative role in the pathogenesis of AI diseases. SLE is characterized by the appearance of abzymes with several different catalytic functions including hydrolysis of peptides and proteins, DNA, RNA, and oligosaccharides. In addition, monoclonal SLE abzymes are characterized by extraordinary diversity in the affinity to the substrates, physicochemical and catalytic characteristics, optimal conditions of catalysis, cytotoxicity, etc. Production of abzymes in SLE mice is associated with changes in the differentiation of hematopoietic stem cells of bone marrow, increase in lymphocyte proliferation, and significant suppression of cell apoptosis in different organs. In this chapter, abzymes with different catalytic activities in SLE are described.

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Section: Catalytic Antibodies In Norm and Systemic Lupus Erythematosusmentioning

confidence: 96%

Section: Lupus 70mentioning

confidence: 99%

Catalytic Antibodies in Norm and Systemic Lupus Erythematosus

Nevinsky¹

2017

Lupus

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321

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“…Natural Abz purification is one of the most complicated aspects of their study; it was discussed in detail in review (Nevinsky et al, 2000 b ). I n s t u d y A b z s f r o m t h e s e r a o f H I V -i n fected patients, electrophoretically and immunologically homogeneous AIDS Ab fraction (pIgG+pIgM+pIgA) was first purified by chromatography of the serum proteins on Protein A-Sepharose under conditions that remove non-specifically bound proteins Baranova et al, 2009Baranova et al, , 2010. pIgMs were separated from pIgAs and pIgGs by FPLC gel filtration of the total Ab fraction on a Superdex 200 (Baranova et al, 2009(Baranova et al, , 2010.…”

Section: Catalytic Antibodies Of Hiv-infected Patientsmentioning

confidence: 99%

“…I n s t u d y A b z s f r o m t h e s e r a o f H I V -i n fected patients, electrophoretically and immunologically homogeneous AIDS Ab fraction (pIgG+pIgM+pIgA) was first purified by chromatography of the serum proteins on Protein A-Sepharose under conditions that remove non-specifically bound proteins Baranova et al, 2009Baranova et al, , 2010. pIgMs were separated from pIgAs and pIgGs by FPLC gel filtration of the total Ab fraction on a Superdex 200 (Baranova et al, 2009(Baranova et al, , 2010. The homogeneity of the 150 kDa IgG was confirmed by SDS-PAGE with silver staining, which showed a single band under nonreducing conditions and two bands corresponding to the H and L chains after reduction (Fig.…”

Section: Catalytic Antibodies Of Hiv-infected Patientsmentioning

confidence: 99%

“…It was shown that non-fractionated on affinity sorbents bearing immobilized DNA or specific protein substrates, pIgGs and pIgMs from HIV infected patients effectively hydrolyze DNA ), HIV-1 RT, human casein, human serum albumin (HAS ;Odintsova et. al., 2006 b ), and HIV-1 IN (Baranova et. al., 2009(Baranova et.…”

Section: Criteria To Establish That Catalytic Activity Is Intrinsic Tmentioning

confidence: 99%

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Natural Catalytic Antibodies in Norm and in HIV-Infected Patients

Nevinsky¹

2011

Understanding HIV/AIDS Management and Care - Pandemic Approaches in the 21st Century

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Autoantibodies in HIV‐infected patients: Cross site‐specific hydrolysis of H1 histone and myelin basic protein

et al. 2018

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Histones act as damage‐associated molecules, while anti‐DNA antibodies are directed against histone‐DNA nucleosomal complexes. Myelin basic protein (MBP) plays an important role in the pathogenesis of multiple sclerosis. Autoantibodies (Abs) with enzymatic activities are the distinctive feature of some autoimmune and viral diseases. Abzymes with proteolytic activity against different proteins specifically hydrolyze only these specific proteins. Using chromatography of IgGs on columns with immobilized H1 histone and then by chromatography of the fraction having an affinity for the histone (eluted upon loading) on MBP Sepharose, the anti‐MBP antibodies were obtained. Anti‐H1 antibodies were obtained using these columns in reverse order. IgGs against H1 and MBP effectively hydrolyze both H1 histone and MBP but no other control proteins. Using the MALDI mass spectrometry, the cleavage sites of H1 histone and MBP by abzymes against these proteins are found. The hydrolysis of MBP by anti‐MBP IgGs occurs at four clusters (22 sites of the hydrolysis) locating at four known antigenic determinants of MBP. Anti‐H1 Abs hydrolyze MBP only at one cluster (11 sites of the hydrolysis); this cluster is only partially overlapped with one of the four MBP clusters. Anti‐H1 antibodies hydrolyze H1 at five sites of one cluster of the protein when anti‐MBP IgGs cleavage this histone at two clusters containing 17 sites of the cleavage. Anti‐H1 and anti‐MBP abzymes are the first examples of Abs possessing not only with cross‐complexing but also with catalytic cross‐reactivity. The existence of cross‐reactivity of abzymes against histones and MBP represent great danger to humans. © 2018 BioFactors, 45(2):211–222, 2019

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HIV-1 integrase-hydrolyzing antibodies from sera of HIV-infected patients

Cited by 48 publications

References 22 publications

Catalytic Antibodies in Norm and Systemic Lupus Erythematosus

Catalytic Antibodies in Norm and Systemic Lupus Erythematosus

Natural Catalytic Antibodies in Norm and in HIV-Infected Patients

Autoantibodies in HIV‐infected patients: Cross site‐specific hydrolysis of H1 histone and myelin basic protein

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