HIV-1 detection in cervicovaginal secretions during pregnancy

Loussert-Ajaka, Ibtissam; Mandelbrot, Laurent; Mc, Delmas; Bastian, Hans; Jl, Bénifla; Farfara, Isabelle; Vincenzi, de; Matheron, Sophie; Simon, François; Brun‐Vézinet, Françoise

doi:10.1097/00002030-199713000-00005

Cited by 43 publications

(18 citation statements)

References 16 publications

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“…This concordance between coshedding of proviral DNA and HIV-1 RNA is in agreement with a previous study of 43 pregnant women and suggests that coshedding of cell-associated and cell-free HIV-1 in the genital tract occurs [28]. The present study also demonstrated a significant correlation between quantity of cell-associated and cell-free HIV-1 within CVL samples; similar findings have been described in nonpregnant women without genital tract infection [31].…”

Section: Discussionsupporting

confidence: 93%

“…The amount of HIV-1 DNA significantly correlated with extent of inflammation and amount of HIV-1 RNA in the CVL, regardless of transmission status. Genital tract infection or inflammation has been associated with increased HIV-1 DNA shedding from the cervix in both pregnant and nonpregnant women [27,28]. Although the relatively few women in the present study limited statistical comparison, the results nevertheless are in concordance with previous studies.…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Genital Tract Human Immunodeficiency Virus Type 1 (HIV‐1) Shedding and Inflammation and HIV‐1envDiversity in Perinatal HIV‐1 Transmission

Panther

Tucker

et al. 2000

J INFECT DIS

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This study sought to identify genital tract characteristics associated with vertical transmission of human immunodeficiency virus type 1 (HIV-1). HIV-1 DNA and RNA, HIV-1 env diversity, and inflammatory cells were quantified in cervicovaginal lavages (CVLs) of 24 women enrolled in the Women and Infants Transmission Study; 7 women transmitted HIV-1 perinatally. Vaginal candidiasis, HIV-1 culture positivity, levels of HIV-1 DNA and cell-free RNA, and HIV-1 env diversity were significantly higher in the CVLs of transmitters. CVL HIV-1 DNA levels correlated with higher levels of inflammatory cells and cell-free HIV-1 RNA. Of subjects with paired blood and CVL specimens, there was more HIV-1 env heterogeneity between blood and CVLs in transmitters than in nontransmitters. In summary, increased HIV-1 shedding is correlated with a more complex population of HIV-1 quasispecies in the genital tracts of parturient women, which may increase the probability that a fetotropic strain is transmitted.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

Genital Tract Human Immunodeficiency Virus Type 1 (HIV‐1) Shedding and Inflammation and HIV‐1envDiversity in Perinatal HIV‐1 Transmission

Panther

Tucker

et al. 2000

J INFECT DIS

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“…The rate of detection of virus was higher (97%) and the median concentrations were lower than those reported elsewhere [2,15]. These differences may be due to differences in the sensitivity of assays used for viral quantitation and differences in sampling methodology.…”

Section: Discussionmentioning

confidence: 67%

Secretory Leukocyte Protease Inhibitor in Vaginal Fluids and Perinatal Human Immunodeficiency Virus Type 1 Transmission

et al. 2001

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The presence of both viral particles and antiviral mucosal proteins may represent critical determinants of perinatal human immunodeficiency virus type 1 (HIV-1) transmission. In 60 HIV-1-infected women, concentrations of the innate mucosal protein, secretory leukocyte protease inhibitor (SLPI), were lower in vaginal fluid samples from 17 women whose babies became infected than in samples from nontransmitting women (mean+/-SE, 57+/-11 vs. 557+/-177 ng/mL, respectively; P=.01). Rates of transmission among women with higher SLPI concentrations (>100 ng/mL) were lower than those among women with lower concentrations (<100 ng/mL; 8.7% vs. 40.5%, respectively; P=.01). Concentrations of other putative HIV-1-inhibitory innate immune factors were similar in both groups. Concentrations of vaginal HIV-1 tended to be higher in transmitting than in nontransmitting women (407 vs. 174 virions/mL; P=.09). Increased concentrations of selected innate mucosal immune factors, such as SLPI, seem to be associated with reduced rates of perinatal HIV-1 transmission and may contribute to natural antiretroviral defense.

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“…Zidovudine, a thymidine NRTI, was the first anti-HIV drug approved by the FDA (De Clercq, 2005). HIV-1 is found in blood, brain, spleen, saliva, lymph nodes, cervicovaginal secretions and semen of infected patients (Wong et al, 1997;Vernazza et al, 1997;Loussert-Ajaka et al, 1997;Pomerantz, 2002). Evidence suggests that anatomically distinct populations of virus exist under different evolutionary selective pressure.…”

Section: Introductionmentioning

confidence: 99%

Rapid and simple liquid chromatography tandem mass spectrometry method for the quantification of zidovudine in rat plasma

Mudigonda

Jukanti

Apte

et al. 2007

Biomedical Chromatography

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A high-performance liquid chromatography/electrospray ionization tandem mass spectrometry method was developed and validated for the quantification of zidovudine in rat plasma. Following solid-phase extraction, the analytes were separated using an isocratic mobile phase on a reverse phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M+H]+ ions, m/z 268/127 for zidovudine and m/z 230/112 for the internal standard. The method exhibited a linear dynamic range of 5-500 ng/mL for zidovudine in rat plasma. The lower limit of quantification was 5 ng/mL with a relative standard deviation of less than 8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 1.5 min for each sample made it possible to analyze more than 400 plasma samples per day. The validated method was applied for pharmacokinetic studies of the novel drug delivery systems of zidovudine in rats.

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HIV-1 detection in cervicovaginal secretions during pregnancy

Abstract: DNA and RNA PCR can be used to detect HIV in cells and supernatants of CVS. These techniques may be useful in cohort studies to investigate HIV transmission and to evaluate the efficacy of antiretroviral drugs to reduce HIV excretion.

Cited by 43 publications

References 16 publications

Genital Tract Human Immunodeficiency Virus Type 1 (HIV‐1) Shedding and Inflammation and HIV‐1envDiversity in Perinatal HIV‐1 Transmission

Genital Tract Human Immunodeficiency Virus Type 1 (HIV‐1) Shedding and Inflammation and HIV‐1envDiversity in Perinatal HIV‐1 Transmission

Secretory Leukocyte Protease Inhibitor in Vaginal Fluids and Perinatal Human Immunodeficiency Virus Type 1 Transmission

Rapid and simple liquid chromatography tandem mass spectrometry method for the quantification of zidovudine in rat plasma

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