History of the progressive development of genetic marker systems for common buckwheat

Yasui, Yukihiko

doi:10.1270/jsbbs.19075

Cited by 13 publications

(5 citation statements)

References 38 publications

(54 reference statements)

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“…Altogether, 89 bands were ampli ed by 16 SSR primers, and 77 of these bands were polymorphic, yielding a proportion of polymorphic loci equal to 86.52%. A total of 74 pairs of alleles were ampli ed, and the largest Na values (7) were found when using primers ESGS125, ESGS155, and ESGS218, with an average of 4.63 alleles per primer. The mean values of Ne, I, Ho, and He were 1.748, 0.700, 0.222, and 0.413, respectively.…”

Section: Polymorphism Analysis Of Molecular Markersmentioning

confidence: 99%

Morphology and SSR markers reveal the genetic diversity of Elymus species germplasm in Northwestern China

Zhang,

Li,

Guo

et al. 2023

Genet Resour Crop Evol

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The relationship between the genetic diversity and genetic characteristics of wild plant germplasm can provide insights for better utilization and conservation of genetic resources. Bunchgrass species in the genus Elymus are important for forage and grassland restoration in Northwest China. In this study, eight phenotypic traits were evaluated in 81 accessions of four Elymus species in the northwest region of China, and genetic diversity analysis was performed using 16 simple sequence repeat (SSR) markers. In the phenotypic trait analysis, Elymus sibiricus had the highest coe cient of variation for single-plant weight(58.24%) and the lowest coe cient of variation for the number of spikelets(0.005%). Clustering based on phenotypic traits placed all varieties into four groups, which were also supported by principal component analysis (PCA). 16 pairs of SSR markers were screened with high polymorphism, with a polymorphism information content (PIC) range of 0.3648-0.7794 and an interspeci c PIC range of 0.465-0.611 in Elymus species. The unweighted pair-group method with arithmetic mean approach applied to SSR marker data also divided the 81 accessions into four groups, similar to the results based on phenotypic traits. The results of PCA and population structure analysis based on SSR data were similar. The ndings presented here will facilitate the collection and conservation of Elymus germplasm resources and provide theoretical references for the future classi cation, breeding, innovation, utilization, and conservation of germplasm.

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Section: Polymorphism Analysis Of Molecular Markersmentioning

confidence: 99%

Morphology and SSR markers reveal the genetic diversity of Elymus species germplasm in Northwestern China

Zhang,

Li,

Guo

et al. 2023

Genet Resour Crop Evol

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“…Currently, 8,884 markers have been proposed, spanning 756 loci. These markers have been used for genomic selection to increase yields (Yasui, 2020).…”

Section: Genomic Analyses Of Buckwheat Using Molecular Markersmentioning

confidence: 99%

Buckwheat – A Genomic, Transcriptomic and Proteomic View

Kučka,

Ražná,

Čerteková

et al. 2023

J microb biotech food sci

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Buckwheat is a pseudocereal from the Polygonaceae family. Two species from this family are commercially exploited – common buckwheat and tartary buckwheat. Buckwheat comes from China, although in recent years, the highest production has been noted in Russia. With its nutritional composition (mainly rutin), it has a beneficial effect on human health. Rutin is mainly contained in the flowers and leaves of buckwheat, and it has antidiabetic, neuroprotective and antioxidant properties; it improves blood pressure and lowers cholesterol levels. In addition to rutin, buckwheat contains bioactive peptides that serve as trypsin inhibitors and have antioxidant and antimicrobial properties. Buckwheat found its use mainly in the field of food and feed production. Amplification polymorphism detection techniques are currently used for the genomic analyses of buckwheat, with 8,884 available markers that include 756 loci. The most frequently used type of molecular markers in buckwheat is the microsatellite markers, which form tandem repeats of short nucleotide motifs. The total number of microsatellites in the tartary buckwheat genome is 37,572, with a frequency of 83.25 microsatellites per 1 Mb. Based on their genetic variability, the buckwheat varieties can be divided into the European and Asian groups, with a lower diversity among the varieties in the European group. Genomic analyses can reveal the genetic relatedness or differences between the individual varieties, as well as losses in genetic purity. The transcriptomic analyses are primarily devoted to the expression of genes responsible for the synthesis of flavonoids, but also those involved in the plant's defense mechanisms, development etc. Molecular analyses revealed that the expression of genes supporting the synthesis of rutin can be favorably influenced by light, darkness, methyl jasmonate, abscisic acid etc. Some buckwheat genes were introduced into Arabidopsis, which subsequently showed improved properties, for example, resistance to drought. These findings not only enhance our understanding of buckwheat at a fundamental level but also hold practical significance for breeding programs focused on enhancing nutritional and agronomic traits in buckwheat varieties.

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“…A major advantage of genomic selection is that, in contrast to the conventional marker-assisted selection, it is suitable for improving quantitative properties managed by multiple quantitative trait loci. Genomic selection and use of DNA markers in breeding buckwheat protein quality are feasible only at the initial stages [ 125 , 126 , 127 ]. Rapid advances in genomic technology will certainly improve genomics-based breeding for buckwheat quality in the near future [ 128 ].…”

Section: Buckwheat Breeding For High Protein Levels: Prospects Formentioning

confidence: 99%

Breeding Buckwheat for Increased Levels and Improved Quality of Protein

Luthar

Zhou

Golob

et al. 2020

Plants

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Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) and common buckwheat (Fagopyrum esculentum Moench) are important sources of proteins with balanced amino-acid compositions, and thus of high nutritional value. The polyphenols naturally present in Tartary buckwheat and common buckwheat lower the true digestibility of the proteins. Digestion-resistant peptides are a vehicle for fecal excretion of steroids, and in this way, for bile acid elimination and reduction of cholesterol concentrations in serum. Buckwheat proteins are more effective compared to soy proteins for the prevention of gallstone formation. Tartary and common buckwheat grain that contains appropriate amounts of selenium-containing amino acids can be produced as functional food products. The protein-rich by-products of buckwheat are a good source of bioactive substances that can suppress colon carcinogenesis by reducing cell proliferation. The grain embryo is a rich source of proteins, so breeding buckwheat with larger embryos is a possible strategy to increase protein levels in Tartary and common buckwheat grain. However, chemical analysis of the grain is the most relevant criterion for assessing grain protein levels and quality.

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History of the progressive development of genetic marker systems for common buckwheat

Cited by 13 publications

References 38 publications

Morphology and SSR markers reveal the genetic diversity of Elymus species germplasm in Northwestern China

Morphology and SSR markers reveal the genetic diversity of Elymus species germplasm in Northwestern China

Buckwheat – A Genomic, Transcriptomic and Proteomic View

Breeding Buckwheat for Increased Levels and Improved Quality of Protein

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