History, epidemiology and diagnostics of dengue in the American and Brazilian contexts: a review

Salles, Tiago Souza; Sá-Guimarães, Thayane da Encarnação; Alvarenga, Evelyn S.L.; Guimarães-Ribeiro, Victor; Meneses, Marcelo Damião Ferreira de; Castro-Salles, Patricia Faria de; Santos, Camilla Ramos dos; Melo, Ana Claudia do Amaral; Soares, Márcia Regina; Ferreira, Davis Fernandes; Moreira, Mônica F.

doi:10.1186/s13071-018-2830-8

Cited by 148 publications

(146 citation statements)

References 31 publications

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“…In 2013, the number of dengue cases in Brazil were estimated in more than 1.4 million, with an economic impact of approximately USD 300 million (about BRL 600 million at the time) 14,15 . The Southeast Region has the highest population among Brazil's five Regions, and also consistently shows the highest number of dengue cases [15][16][17] . Contrarily, the incidence rates in southern Brazil are consistently low when compared to other regions, especially the Central-West Region, which presents the highest incidence of dengue infection per capita [15][16][17] .…”

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confidence: 99%

“…The Southeast Region has the highest population among Brazil's five Regions, and also consistently shows the highest number of dengue cases [15][16][17] . Contrarily, the incidence rates in southern Brazil are consistently low when compared to other regions, especially the Central-West Region, which presents the highest incidence of dengue infection per capita [15][16][17] . The South Region is formed by three states: Paraná, Santa Catarina and Rio Grande do Sul, and it represents 14% of the country's population 18 .…”

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confidence: 99%

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Epidemiological study on dengue in southern Brazil under the perspective of climate and poverty

Bavia

Melanda

Arruda

et al. 2020

Sci Rep

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Social and epidemiological aspects of dengue were evaluated in an important metropolitan area in southern Brazil, from August 2012 to September 2014. Demographic, clinical, serological data were collected from patients with acute dengue symptoms treated at public health system units (HSUs). A systematic approach to analyze the spatial and temporal distribution of cases was developed, considering the temporal cross-correlation between dengue and weather, and the spatial correlation between dengue and income over the city's census tracts. From the 878 patients with suggestive symptoms, 249 were diagnosed as positive dengue infection (28%). Considering the most statistically significant census tracts, a negative correlation was found between mean income and dengue (r = −0.65; p = 0.02; 95% CI: −0.03 to −0.91). The occurrence of dengue followed a seasonal distribution, and it was found to be three and four months delayed in relation to precipitation and temperature, respectively. Unexpectedly, the occurrence of symptomatic patients without dengue infection followed the same seasonal distribution, however its spatial distribution did not correlate with income. Through this methodology, we have found evidence that suggests a relation between dengue and poverty, which enriches the debate in the literature and sheds light on an extremely relevant socioeconomic and public health issue.

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Epidemiological study on dengue in southern Brazil under the perspective of climate and poverty

Bavia

Melanda

Arruda

et al. 2020

Sci Rep

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“…For rapid and accurate diagnosis of dengue virus in early phases of acute infections, detection of DENV RNA is recommended as the most sensitive and specific method to diagnose dengue in the acute phase of the illness by WHO [9]. After the first developed nucleic acid detection test for dengue diagnosis over 20 years ago [15], there have been a number of RT-PCR assays for DENV detection and serotype identification with high sensitivity and specificity [16][17][18][19][20]. Combined with automated RNA extraction protocols, RT-PCR can potentially facilitate fast and accurate diagnosis of dengue suspected cases with a single sample obtained during the patient's first visit to the clinician.…”

Section: Introductionmentioning

confidence: 99%

A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos

Tsai¹,

Liu²,

Lin³

et al. 2019

PLoS ONE

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Background The insulated isothermal PCR (iiPCR) technology enables consistent PCR amplification and detection in a simple heating device. A pan-dengue virus (DENV) RT-iiPCR, targeting the 5’ untranslated region, was validated previously on the semi-automated POCKIT combo system (involving separate devices for nucleic acid extraction and PCR amplification/detection) to offer performance comparable to a laboratory real-time PCR. Working on the same technologies, a compact automated sample-in-answer-out system (POCKIT Central Nucleic Acid Analyser) has been available commercially for iiPCR, minimizing human error risks and allowing easy molecular bio-detection near points of need. Here, we evaluated the analytical and clinical performance of the pan-DENV RT-iiPCR on the fully automated system by comparison to those on the semi-automated system. Methodology/Principal findings Testing sera containing serial diluted DENV-1, -2, -3, or -4 cell culture stock, the pan-DENV RT-iiPCR system had similar 100% detection endpoints on the two systems; i.e. at 1, 10, 1 and 10 PFU/ml, respectively, on the fully automated system, and at 10, 1, 10 and 10 PFU/ml, respectively, on the semi-automated system. Furthermore, both fully automated and semi-automated PCR system can detect all four DENV serotypes in mosquitos. Clinical performance of the reagent on the two systems was evaluated by testing 60 human serum samples. Both systems detected the same 40 samples (ten DENV-1, -2, -3, and -4 positive each) and did not detect the other 20; 100% agreement (κ = 1) was found between the two systems. Conclusions/Significance With performance comparable to a previously validated system, the fully-automated PCR system allows applications of the pan-DENV reagent as a useful tool near points of need to facilitate easy, fast and effective detection of dengue virus and help mitigate versatile public health challenges in the control and management of dengue disease.

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“…De maneira específica, nos últimos 50 anos, junto com o aumento da expansão geográfica, a incidência dessa doença aumentou aproximadamente 30 vezes. Além disso, ocorrem anualmente cerca de 390 milhões de infecções por dengue, sendo que 96 milhões dos indivíduos acometidos apresentam manifestações clínicas 1,2 .…”

Section: Introductionunclassified

Avaliação acerca do conhecimento sobre a Dengue em jovens em idade escolar

et al. 2019

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Introdução: A dengue é uma arbovirose, transmitida para o homem através da picada do mosquito Aedes aegypti, e que acomete grande número de indivíduos no mundo. Nos últimos 50 anos, junto com o aumento da expansão geográfica, a incidência de dengue aumentou aproximadamente 30 vezes. Ao longo dos anos, diversas medidas de combate à dengue foram implementadas, a partir daí, no ano de 2004 foi possível evidenciar que houve redução de 290% nos casos de dengue em relação ao ano anterior. Objetivo: Descrever o conhecimento de escolares do Rio Grande do Sul sobre o tema dengue. Métodos: Estudo com abordagem quantitativa do tipo descritiva realizado com jovens alunos da rede pública de educação da cidade de Tupãnciretã, por meio de um questionário estruturado. Resultados: Evidenciou-se que a maioria dos alunos tinha conhecimento sobre como a dengue é transmitida, sendo que 35% acreditavam que a fêmea do mosquito transmite a doença e ainda, 58,8% afirmaram que conseguem reconhecer o mosquito. Uma parcela dos alunos acredita que o mosquito pica preferencialmente durante o dia, onde a principal forma de transmissão selecionada foi “O mosquito pica uma pessoa doente e transmite a doença à outra pessoa” e os principais sintomas assinalados foram febre, dor de cabeça e cansaço. Conclusão: O estudo demonstrou que boa parte dos jovens possuem conhecimentos sobre a dengue, entretanto, ainda há crianças e adolescentes que não conhecem o assunto, trazendo à tona a importância deste tema nas campanhas de saúde.

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History, epidemiology and diagnostics of dengue in the American and Brazilian contexts: a review

Cited by 148 publications

References 31 publications

Epidemiological study on dengue in southern Brazil under the perspective of climate and poverty

Epidemiological study on dengue in southern Brazil under the perspective of climate and poverty

A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos

Avaliação acerca do conhecimento sobre a Dengue em jovens em idade escolar

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