History, efficacy, orchard ecology, and mode of action of Aureobasidium pullulans, the microbial agent in Blossom Protect, for suppression of fire blight of pome fruit

Kunz, Stefan; Zeng, Quan; Johnson, K. B.

doi:10.1007/s42161-023-01448-4

J Plant Pathol

2023

DOI: 10.1007/s42161-023-01448-4

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History, efficacy, orchard ecology, and mode of action of Aureobasidium pullulans, the microbial agent in Blossom Protect, for suppression of fire blight of pome fruit

Stefan Kunz¹,

Quan Zeng

K. B. Johnson

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Cited by 6 publications

(1 citation statement)

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“…A. pullulans is well-known for its significant biotechnological potential in medicine, pharmacy, and the food industry, which has been attributed to its capacity to produce polymeric substances, particularly pullulan, and hydrolytic enzymes [2,3]. Additionally, this fungus has been used as a commercial biocontrol agent against postharvest diseases and fire blight [4,5]. Notably, strain MHAU2101 was recently isolated from pears in Korea and demonstrated great efficacy in controlling fire blight caused by Erwinia amylovora [6].…”

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confidence: 99%

Draft Genome Sequence of Aureobasidium pullulans Strain MHAU2101, a Biological Control Agent against Fire Blight from Korea

He,

Luo,

Lee

et al. 2023

Microbiol. Biotechnol. Lett.

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Aureobasidium pullulans is often referred to as "yeastlike" or "black yeast" due to its melanized yeast-like morphology. This fungus is ubiquitous in a variety of environments and is frequently isolated from the phyllosphere and soil [1]. A. pullulans is well-known for its significant biotechnological potential in medicine, pharmacy, and the food industry, which has been attributed to its capacity to produce polymeric substances, particularly pullulan, and hydrolytic enzymes [2,3]. Additionally, this fungus has been used as a commercial biocontrol agent against postharvest diseases and fire blight [4,5]. Notably, strain MHAU2101 was recently isolated from pears in Korea and demonstrated great efficacy in controlling fire blight caused by Erwinia amylovora [6]. The fungal strain MHAU2101 was identified as A. pullulans by morphological and phylogenetic analyses, employing large-subunit 26S ribosomal DNA and the internal transcribed spacer [6].In this study, we sequenced the genome of A. pullulans MHAU2101, isolated from pear flowers in Wanju, South Korea, in 2021 [6]. The genomic DNA of strain MHAU2101 was extracted using the Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, USA). DNA purity and content were evaluated by spectrophotometry (NanoPhotometer NP80, Implen, Germany), and DNA integrity was assessed with electrophoresis. The quality-controlled DNA was used for genome sequencing using a combination of the PacBio Sequel and Illumina NovaSeq sequencing platforms, and the PacBio SMRTbell prep kit 3.0 (PacBio, USA) and TruSeq Nano DNA Preparation Kit (350) (Illumina, USA) were used to construct two sequencing libraries, respectively. Sequencing was performed by SEEDERS Co. (Republic of Korea; http:// www.seeders.co.kr/). The Illumina data were used to polish the assembled genome using PacBio data and Pilon v1.21 software [7]. The benchmarking universal single-copy orthologs (BUSCO v5.1.3) dataset eukaryota_In this study, we present the draft genome of Aureobasidium pullulans strain MHAU2101, which is the first strain to effectively control fire blight caused by Erwinia amylovora in Korea. The genome of strain MHAU2101 was composed of 28,669,322 base pairs, with a C+G content of 50.4%. The assembly comprised 17 contigs and had 99.22% completeness. The results of this study will be a valuable resource for future research on the biocontrol mechanism of A. pullulans strain MHAU2101.

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Draft Genome Sequence of Aureobasidium pullulans Strain MHAU2101, a Biological Control Agent against Fire Blight from Korea

He,

Luo,

Lee

et al. 2023

Microbiol. Biotechnol. Lett.

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Burning questions for fire blight research. II. Critical next steps in disease management and in host resistance breeding of apple and pear

Zeng,

Emeriewen,

Rezzonico

et al. 2024

J Plant Pathol

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A new strain Lacticaseibacillus paracasei M12 and perspectives of its use against Erwinia amylovora

Sadanov,

Ismailova,

Shemshura

et al. 2024

J Plant Pathol

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History, efficacy, orchard ecology, and mode of action of Aureobasidium pullulans, the microbial agent in Blossom Protect, for suppression of fire blight of pome fruit

Cited by 6 publications

References 62 publications

Draft Genome Sequence of Aureobasidium pullulans Strain MHAU2101, a Biological Control Agent against Fire Blight from Korea

Draft Genome Sequence of Aureobasidium pullulans Strain MHAU2101, a Biological Control Agent against Fire Blight from Korea

Burning questions for fire blight research. II. Critical next steps in disease management and in host resistance breeding of apple and pear

A new strain Lacticaseibacillus paracasei M12 and perspectives of its use against Erwinia amylovora

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