“…To evaluate the role of repair and immobilization/activity (IM/ACT) 6-weeks post-injury, animals were randomized into repaired or non-repaired groups, and further randomized into groups immobilized for 1, 3, or 6 weeks that later resumed cage and activity for 5, 3, or 0 weeks (IM1/ACT5, IM3/ACT3, IM6/ACT0) (Figure 1). Repairs were performed with the Urbaniak variant of the Kessler repair 23 using 4-0 Tevdek (PTFE coated) suture, as done previously. 12 Immobilization procedures consisted of casting the leg from below the knee to the toes in a fully plantarflexed position using silk tape stirrups (3M; St. Paul, MN), webril padding (Hanna Pharmaceutical; Wilmington, DE), a plastic splint (MakerBot Industries, LLC; Brooklyn, NY), CoFlex (Andover Healthcare; Salisbury, MA), and poly(methyl-methacrylate) (Patterson Dental; St. Paul, MN).…”