Historical Review and Technical Survey of Vascular Casting and Scanning Electron Microscopy

Lametschwandtner, Alois; Lametschwandtner, U

doi:10.1007/978-1-4615-3488-4_1

Cited by 29 publications

(23 citation statements)

References 14 publications

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“…In addi tion. the materials we used for the casts were relatively nontoxic to the tissues and blood vessels [25], Thus, hypertension induces cap illary network damage, although this change can hardly be confirmed clinically due to the fact that the capillary network is composed of two layers in the deep retina.…”

Section: Discussionmentioning

confidence: 99%

Vascular Changes in Retinas of Spontaneously Hypertensive Rats Demonstrated by Corrosion Casts

Bhutto¹,

Amemiya²

1997

Ophthalmic Res

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The changes in the capillary network of the retina in spontaneously hypertensive rats (SHR) were demonstrated by scanning and transmission electron microscopy. Corrosion casts with scanning electron microscopy provided a three-dimensional view of the microvasculature of the rat retina and could detect the conspicuous features of the retinal vasculature in SHR. In general, when hypertension lasted for 6–7 months in SHR, tortuosity of the retinal vessels was noted, and later the SHR retina showed increased tortuosity and generalized narrowing of the vessels, localized constriction of the veins, arteriovenous crossing defects, and marked capillary changes, such as caliber irregularity, narrowing, bead-like capillaries, loop formation and localized obliteration. At a later stage, when the blood pressure had been sustained for a long time, there was marked capillary constriction which first affected the superficial capillary network, then scattered capillary network constriction and finally capillary dropout. Transmission electron microscopy revealed narrow capillary lumina and thin endothelium with scarce cytoplasmic components and damaged pericytes. These morphological changes in the capillary network were probably due to structural damage to the endothelial cells, facilitated possibly by compression of the precapillary arterioles. The severity of these changes was usually proportional to the degree and duration of hypertension. These findings indicate that the retinal capillary changes are probably related to hypertension.

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Section: Discussionmentioning

confidence: 99%

Vascular Changes in Retinas of Spontaneously Hypertensive Rats Demonstrated by Corrosion Casts

Bhutto¹,

Amemiya²

1997

Ophthalmic Res

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“…Individual dry carotid labyrinths were either mounted in toto onto specimen stubs for detailed SEM analyses, or were cut longitudinally or transversely by a razor blade before mounting. Some carotid labyrinths were submerged into distilled water, frozen and sectioned while embedded in ice using a mini-wheel saw placed in the cryochamber of a cryo-microtome (Lametschwandtner and Lametschwandtner, 1992). Transverse or longitudinally sectioned casts where cleaned, air-dried, mounted and finally analyzed in the SEM.…”

Section: Juveniles and Adultsmentioning

confidence: 99%

Vascular architecture of the carotid labyrinth in larval and adult Xenopus laevis – Histomorphology and scanning electron microscopy of vascular corrosion casts

Deutschmann¹,

Minnich²,

Lametschwandtner³

2017

Archives of Histology and Cytology

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“…After the microvascular anatomy of the gallbladders had been documented in-situ, gallbladders were removed, mounted onto new stubs, coated, and previously hidden areas were documented. Finally, gallbladders were removed from the specimen stubs, frozen in distilled water, and sectioned transversely, longitudinally, tangentially or horizontally with a mini wheel-saw placed in the chamber of a cryomicrotome (Lametschwandtner & Lametschwandtner 1992). Sectioned ice-embedded specimens were allowed to thaw in distilled water, cleaned, and refrozen in bidistilled water for subsequent freezedrying (Lyovac GT 1, Leybold-Hereaus; Cologne, Germany).…”

Section: Vascular Castingmentioning

confidence: 99%

The microvasculature of the gallbladder in adultXenopus laevis(Amphibia, Anura): A scanning electron microscope study of vascular corrosion casts and correlative light microscopy

Lametschwandtner

Bartel

Minnich

et al. 2011

Italian Journal of Zoology

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Microvasculature, anatomy and histomorphology of the gallbladder of the adult pipid frog, Xenopus laevis (Daudin) were studied by light (LM) and scanning electron microscopy (SEM) of tissue preparations and vascular corrosion casts. The thin wall of the gallbladder consisted of columnar epithelium, lamina propria, a fibromuscular layer, subserosa and serosa. A few diverticula but no epithelial folds were present. Up to five cystic arteries arose from the adjacent right hepatic artery and approached the gallbladder via its neck region. One to two cystic veins departed from the bottom and drained into the nearby abdominal vein. Cystic arteries were of the pinnate-type and embraced the gallbladder with their branches. Terminal arterioles gave rise to wide subepithelial capillaries. Transition distances from arteries to veins were short. In general, three to four layers of blood vessels built the vascular bed of the gallbladder wall. The wide subepithelial capillaries established an interface well adapted (1) to allow capillary circulation over a wide range of filling states of the gallbladder and (2) to serve the exchange processes taking place between stored bile and the blood vascular system via the microvillous columnar epithelium.

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Historical Review and Technical Survey of Vascular Casting and Scanning Electron Microscopy

Cited by 29 publications

References 14 publications

Vascular Changes in Retinas of Spontaneously Hypertensive Rats Demonstrated by Corrosion Casts

Vascular Changes in Retinas of Spontaneously Hypertensive Rats Demonstrated by Corrosion Casts

<b>Vascular architecture of the carotid labyrinth in larval and adult </b><i><b>Xenopus laevis</b></i><b> – Histomorphology and scanning electron microscopy of vascular corrosion </b><b>casts </b>

The microvasculature of the gallbladder in adultXenopus laevis(Amphibia, Anura): A scanning electron microscope study of vascular corrosion casts and correlative light microscopy

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