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A gap exists between good laboratory practises with axenic animals and procedures applied. This work aimed at choosing the appropriate disinfectant between sodium dichloroisocyanurate (MB-10) and potassium peroxymonosulfate (Virkon<sup>™</sup>) disinfectants and to adjust the soaking time of the material used with ISOcage biosafety stations. Another aim was to compare the microbial load on cage systems hosting mice since two weeks in axenic (AR) rooms and typical specific-pathogen-free (SPF) non-axenic rooms (NAR) to identify resistant microorganisms targeted for longer soaking disinfection as well as evaluate microbial concentration reduction procedures in AR. <i>Staphylococcus</i> was the most frequently isolated genus (AR and NAR). An average of three spore-forming microorganisms per cage were counted from AR. The disinfection time to reach 1 log reduction for <i>Bacillus atrophaeus</i> spores varied from 138 (100 ppm MB-10) to 290 (Virkon<sup>™</sup>) seconds and below 20 seconds for <i>S. epidermidis</i> (100 ppm MB-10). AR management protocols lead to 1000 times lower microorganisms burden compared to NAR. Data comparing microbial load on SPF and axenic facilities can be used as comparison for facilities aiming at improving the effectiveness of their microbial control procedures.
A gap exists between good laboratory practises with axenic animals and procedures applied. This work aimed at choosing the appropriate disinfectant between sodium dichloroisocyanurate (MB-10) and potassium peroxymonosulfate (Virkon<sup>™</sup>) disinfectants and to adjust the soaking time of the material used with ISOcage biosafety stations. Another aim was to compare the microbial load on cage systems hosting mice since two weeks in axenic (AR) rooms and typical specific-pathogen-free (SPF) non-axenic rooms (NAR) to identify resistant microorganisms targeted for longer soaking disinfection as well as evaluate microbial concentration reduction procedures in AR. <i>Staphylococcus</i> was the most frequently isolated genus (AR and NAR). An average of three spore-forming microorganisms per cage were counted from AR. The disinfection time to reach 1 log reduction for <i>Bacillus atrophaeus</i> spores varied from 138 (100 ppm MB-10) to 290 (Virkon<sup>™</sup>) seconds and below 20 seconds for <i>S. epidermidis</i> (100 ppm MB-10). AR management protocols lead to 1000 times lower microorganisms burden compared to NAR. Data comparing microbial load on SPF and axenic facilities can be used as comparison for facilities aiming at improving the effectiveness of their microbial control procedures.
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