Aim of this thesis is to present an experimental investigation of standard R744 supermarket refrigeration system, with the high-pressure electronic valve (HPV), and refrigeration system with multi-ejector expansion pack on the same vapour compression rack. Comparison of the R744 multi-ejector refrigeration system, was carried out based on energy performance characteristics: refrigeration capacity, power consumption, COP, and exergy efficiency. Apart from the system performance comparison, influence of the pressure level in the flash tank on the system performance for both alternatives was analysed.The experimental results indicated COP and exergy efficiency improvement of the multiejector refrigeration system up to 7% and 13.7%, respectively. The multi-ejector system was able to operate in smaller range of the tanks pressure lift than the standard system dependent on the refrigeration load and the exit gas cooler section parameters. The highest values of COP and exergy efficiency were obtained by the multi-ejector refrigeration system for the tanks pressure lift value close to the limit value. The values of the overall compressor efficiencies were significantly differentiated, dependent on the operation module (cooling load and heat rejection conditions), which strongly influenced the values of COP and exergy efficiency. Therefore, it was not possible to clearly define the optimum pressure in the flash tank. It was concluded that improvement of compressors efficiencies utilized in the multiejector system will indicate high energy performance of the refrigeration system.