Histoplasma capsulatum fungemia in patients with acquired immunodeficiency syndrome: detection by lysis-centrifugation blood-culturing technique

Oliveira, Flávio de Mattos; Fernandes, Sérgio Sônego; Severo, Cecília Bittencourt; Guazzelli, Luciana Silva; Severo, Luiz Carlos

doi:10.1590/s0036-46652007000300001

Cited by 10 publications

(8 citation statements)

References 17 publications

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“…Aspecto curioso do presente estudo foi o achado de hemoculturas positivas em 8 (13,3%) pacientes com micose sistêmica. Esse aspecto tem sido associado a pior prognóstico da criptococose 31 38 e histoplasmose 28 . Também, para a paracoccidioidomicose, há relato de sepse grave seguida de morte, na qual o fungo foi isolado de hemocultura 4 .…”

Section: Discussionunclassified

Micoses sistêmicas: fatores associados ao óbito em pacientes com infecção pelo vírus da imunodeficiência humana, Cuiabá, Estado de Mato Grosso, 2005-2008

Ribeiro

Hahn

Favalessa

et al. 2009

Rev. Soc. Bras. Med. Trop.

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Mesmo com a queda progressiva da incidência de infecções oportunistas, após o advento da moderna terapia antirretroviral potente ou high activity antiretroviral therapy (HAART) 17 , estas complicações ainda constituem a principal causa de morbidade e mortalidade para a população portadora do vírus da imunodeficiência humana (Aids) resultando muitas vezes em hospitalização, e requerendo, em algumas situações, tratamentos

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Section: Discussionunclassified

Micoses sistêmicas: fatores associados ao óbito em pacientes com infecção pelo vírus da imunodeficiência humana, Cuiabá, Estado de Mato Grosso, 2005-2008

Ribeiro

Hahn

Favalessa

et al. 2009

Rev. Soc. Bras. Med. Trop.

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“…Tests for detecting microorganisms in blood cultures are needed in cases of renal involvement, osteoarticular lesions, endocarditis, meningitis, hypotension and multiple organ failure and other factors 14 .…”

Section: Discussionmentioning

confidence: 99%

“…In most cases, latent foci of histoplasmosis, may culminate in disseminated form. 14,22 . According to Borges 22 , 18 cases of histoplasmosis occurred in immunosuppressed patients, and 17 had AIDS, mostly males.…”

Section: Discussionmentioning

confidence: 99%

Fungemia in a university hospital: an epidemiological approach

Couto

Macedo

Neves

2011

Rev. Soc. Bras. Med. Trop.

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Introduction: Fungemia corresponds to the isolation of fungi in the bloodstream and occurs mostly in immunosuppressed patients. The early diagnosis and treatment of these infections are relevant given the serious threat to the affected patients and possible spread to other organs, often becoming fatal. The growing number of fungemia associated with poor prognosis resulted in this research aiming to diagnose and assess the epidemiological aspects of hematogenous infections by fungi. Methods: The study included 58 blood samples collected within a 1-year period, from patients at the Hospital das Clinicas, Federal University of Pernambuco, by venipuncture in vacuum tubes. Blood samples were processed for direct examination and culture and identification, conducted by observing the macroscopic and microscopic characteristics, as well as physiological characteristics when necessary. Results: Eight (13.8%) episodes of fungemia were identified, accounting for the total sample, and these pathogens were Candida, Histoplasma, Trichosporon, Cryptococcus, and a dematiaceous fungus. C. albicans was the prevalent species, accounting for 37.5% of the cases. Most affected patients were adult males. There was no predominance for any activity, and the risk of acquired immunodeficiency syndrome was the underlying pathology most often cited. Conclusions: The isolation of fungi considered as emergent species, such as C. membranifaciens and dematiaceous species, highlights the importance of epidemiological monitoring of cases of fungemia in immunocompromised patients, as the therapy of choice depends on the knowledge of the aethiological agent.

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“…Consequently, Isolator should be used in cases of suspected disseminated disease due to sensitivity and reduced mean time for detection of positive culture [64,65]. In suspected cases of bone marrow infection, if the blood cultures is done by lysis-centrifugation, the aspiration procedures is not necessary [65][66][67][68][69].…”

Section: Blood Culture Systemsmentioning

confidence: 99%

Updating: Cryptococcosis Diagnostic Aspects

2014

J AIDS Clin Res

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Currently, AIDS represents the most common risk factor for the development of cryptococcosis. Despite of rapid scale-up of antiretroviral therapy programs and global investement in HIV care over the past decade, the decline in incidende and mortality rates associated with cryptococcosis has not been commensurate. This article puts into context recent diagnostics studies and morphologies-with special attention to laboratory diagnosis based on classical mycology: (a) histopathology through the histochemical techniques of hematoxilin and eosin, periodic acid-Schiff, and Grocott's silver, as well special histochemical techniques such as Mayer's mucicarmine and Fontana-Masson; (b) cultures by Sabouraud's medium, brain-heart infusion agar, canavanineglycine-bromothymol blue agar, in addition to niger seed agar medium and lysis-centrifugation blood-culturing technique; (c) serology by cryptococcal antigen test and cryptococcal antigen lateral flow assay. In this paper, we also discussed the new rapid point-of-care lateral flow assay to be used to screening HIV-infected person with CD4 counts of less than 100 cells /µL. It is noteworthy that laboratory findings should be interpreted considering the clinical and epidemiological history of all patients. Consequently, diagnostic tools are only properly used, if the treating physician is aware of the propensity of patients to acquire a fungal infection.

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Histoplasma capsulatum fungemia in patients with acquired immunodeficiency syndrome: detection by lysis-centrifugation blood-culturing technique

Cited by 10 publications

References 17 publications

Micoses sistêmicas: fatores associados ao óbito em pacientes com infecção pelo vírus da imunodeficiência humana, Cuiabá, Estado de Mato Grosso, 2005-2008

Micoses sistêmicas: fatores associados ao óbito em pacientes com infecção pelo vírus da imunodeficiência humana, Cuiabá, Estado de Mato Grosso, 2005-2008

Fungemia in a university hospital: an epidemiological approach

Updating: Cryptococcosis Diagnostic Aspects

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