Histopatological alterations and oxidative stress in liver and kidney of Leuciscus cephalus following exposure to heavy metals in the Tur River, North Western Romania

Hermenean, Anca; Damache, Georgiana; Albu, Paul; Ardelean, Aurel; Ardelean, Gavril; Ardelean, Dorina; Horge, Monica; Nagy, Tibor; Braun, Mihály; Zsuga, Miklós; Kéki, Sándor; Costache, Marieta; Dinischiotu, Anca

doi:10.1016/j.ecoenv.2015.05.029

Cited by 81 publications

(46 citation statements)

References 49 publications

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“…The present study showed the reverse tendency between SOD, CAT activities with GPx activity for treated groups at 7 days, especially in treated groups at 14 days in the present study, which demonstrated that CAT activity could be compensated by a high decrease of the GPx activity (Atli and Canli 2011 ). GST activity was also significantly induced at 5 mg/L MT groups, which in agreement with the study observed in Leuciscus cephalus exposed to heavy metals (Hermenean et al 2015 ). The decreased total GSH observed in the present study was the same as methylmercury-exposed in rainbow trout (Mozhdeganloo et al 2015 ), while different from waterborne lead exposure for tilapia (Kaya and Akbulut 2015 ).…”

Section: Discussionsupporting

confidence: 91%

Effect of 17α-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus

et al. 2016

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The normal dose of 17α-methyltestosterone (MT) used in fish farming was 60 mg/L, and now the analysis of residual androgens was carried out in waste water obtained from the Beijing area, which could be detected in levels ranging from 4.1 to 7.0 ng/L. For the purpose of aquatic early warning, the present study clearly demonstrated that chronic exposure by higher concentration of MT than environmental relevant concentrations could trigger oxidative stress response to juvenile tilapia by modulating hepatic antioxidant enzyme activities and gene transcription. Some antioxidative parameters (T-GSH, GSH/GSSG and MDA) were significant decreased under 0.5 mg/L MT exposure at 7 and 14 days. Some antioxidant enzymes (SOD, CAT and GST) and transcriptional changes (sod and cat) were revealed significant decreases for MT treated groups at 7 days. Total antioxidant capacity was significant increased only in 5 mg/L MT exposure groups, but GR activities were not affected all through the whole exposure period. Almost all of the antioxidant enzymatic genes detected in the present study were showed significant increments for MT exposure both at 14 and 21 days, and the genotoxicity profile of antioxidant enzymatic genes were revealed dose-dependent manner. This study presented evidence that MT could result in oxidative stress response in the early stages of GIFT tilapia.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-1946-6) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 91%

Effect of 17α-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus

et al. 2016

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“…Increased GST activity has been previously observed in Leuciscus cephalus exposed to heavy metals, which was related to milder structural changes observed in the liver compared to the kidney (Hermenean et al, 2015). GST activity was also significantly induced in the liver of fish exposed to PCBs in this study, and the liver showed congestion only for 5 mg/L PCB-exposure groups at 21 days in the present study (Figure 1).…”

Section: Resultssupporting

confidence: 60%

Effect of polychlorinated biphenyls on oxidation stress in the liver of juvenile GIFT, Oreochromis niloticus

et al. 2016

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“…It has been reported previously that the exposure to toxic concentrations of Cd causes a decline in cellular pool of GSH and thiol containing proteins in mollusc, 40,43 and freshwater fish. 44 A dose dependent decline in GSH:GSSG ratio in response to Cd exposure was also observed in a mammalian renal cell line. 45 GSH can scavenge free ionic species of Cd (Cd 2+ ), which is the main driver for Cd cytotoxicity.…”

Section: Discussionmentioning

confidence: 85%

An in vitro examination of selenium–cadmium antagonism using primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes

2016

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The present study evaluated the ameliorative properties of selenium (Se) against cadmium (Cd)-induced oxidative stress, using isolated rainbow trout (Oncorhynchus mykiss) hepatocytes in primary culture as the model experimental system. Cadmium (Cd) is known to induce cytotoxic effects by disrupting cellular oxidative homeostasis. On the other hand, selenium (Se) is an essential component of biological antioxidative machinery, and thus may provide protection against the toxic insults of Cd by augmenting the cellular antioxidant response. However, Se, when present above the threshold concentration, can also induce reactive oxygen species (ROS) generation and cause oxidative damage. In this experiment, trout hepatocytes in primary culture were exposed to 100 µM Cd, alone or in combination with different concentrations (25-500 µM) of selenite (SeO3(2-)) or selenomethionine (SeMet) for 48 h. Our findings indicated that both chemical forms of Se, at the lowest concentration used (25 µM), significantly reduced Cd-induced cytotoxicity (measured as cell viability). In contrast, Se at higher concentrations (≥ 50 µM) did not offer any protection against a Cd induced decrease in cell viability. The reduced cytotoxicity of Cd in the presence of 25 µM selenite or SeMet was associated with reduced intracellular ROS production, recovery of the cellular thiol status (ratio of reduced and oxidized glutathione), and amelioration in the activities of major enzymatic antioxidants (superoxide dismutase, catalase, and glutathione peroxidase). Co-treatment of hepatocytes with Cd and pharmacological antioxidants (TEMPO and NAC) also reduced Cd-induced oxidative stress in trout hepatocytes. This provided further evidence that Se likely ameliorates Cd toxicity via different antioxidative mechanisms.

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Histopatological alterations and oxidative stress in liver and kidney of Leuciscus cephalus following exposure to heavy metals in the Tur River, North Western Romania

Cited by 81 publications

References 49 publications

Effect of 17α-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus

Effect of 17α-methyltestosterone (MT) on oxidation stress in the liver of juvenile GIFT tilapia, Oreochromis niloticus

Effect of polychlorinated biphenyls on oxidation stress in the liver of juvenile GIFT, Oreochromis niloticus

An in vitro examination of selenium–cadmium antagonism using primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes

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